Answered: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is the total length of the DNA fragment?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN: 9780134580999

Author: Elaine N. Marieb, Katja N. Hoehn

Publisher: PEARSON

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Question

Above are the results of gel electrophoresis following digestion with restriction enzymes. What is the total length of the DNA fragment?

Which enzyme, HindIII or EcoRI, produced a larger fragment?

What part of DNA causes it to be negatively charged in order for electrophoresis to work?

100
-
T |
I
I |||
Hindill digestion
EcoRI digestion
Hindi/EcoRI
Double digestion

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Step 1: Defining restriction digestion

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Step 2: 1. Calculating the length of the DNA fragment

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Step 3: 2. Finding the larger fragment

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Step 4: 3. Understanding the electrophoresis

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