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- Activity 1.4: Essay.Direction: Explain your answer.Today, it is easy to make transgenic plants and animals. What are some important safety and ethical issues raised by this use of recombinant DNA technology? Explain your answer.The following are words used in animal testing and its alternative methods. Define the following terms - a. Vivisection b. Optogenics c. In vivo d. In vitro cell culture e. In silico computer simulation f. Pharmacokinetics g. Pharmacodynamics h. Tissue culture i. Phototoxicity j. 3D cell culturesMatch the following experimental methods with their specialty procedures and objectives. autoradiography use of green fluorescent protein (GFP) fractional centrifugation use of genetic mutants A. silencing genes means inhibition of the expression of a protein B. fluorescing GFP protein is fused with a protein tracked in the cell C. biochemical processes are visualized using radiolabeled materials D. tissue samples homogenized at varying centrifugal force leads to isolation of desired cellular fraction
- PLEASE HELP ME ANSWER THESE OBJECTIVES QUESTIONS. THANK YOU IN ADVANCE. 1. In genetic engineering, a reverse transcriptase enzyme is used to a. replace the Pfu polymerase enzyme b. denature all messenger RNAs c. undo the action of a restriction enzyme d. produce complementary DNA 2. In lac operon, both gene A and gene B undergo a transcription process. Gene B can only undergo transcription in the presence of lactose and in the absence of glucose. The product of gene A is often altered by an inducer. Which of the following is true about genes A and B? a. Gene A= structural gene; Gene B= regulatory gene b. Gene A= promoter gene; Gene B= operator gene c. Gene A= lacZ gene; Gene B= promoter gene d. Gene A= regulatory gene; Gene B= structural gene 3. In response to a newly encountered pathogen, the first antibody class to appear in the serum is ________. a. IgE b. IgM c. IgA d. IgG 4. Lymphocytes that involve in the defense system by means of direct attack…Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: a. b. Deoxynucleotide triphosphates (dNTPs) Answer: Forward and reverse primers Answer: с. Task 3: Agarose Gel Electrophoresis Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) GDNA extract, (D) PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane is which. You are shown a diagram of the obtained gel below. a. Label each lane of the gel. Write only the corresponding letters in the wells above. b. Above each band in the size ladder, write its size (in kb). c.…Topic: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting factors) Question When or why is this genetic technology/process used? Who benefits from this genetic technology/process - and how?
- Topic: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting factors) Question What are the drawbacks/disadvantages/unknowns associated with this genetic process?Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. Show some sample computation. Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319Please offer a clear explanation of the ChIP assay. A. What characteristics of these cells are being assessed? Why would this be valuable to a research team? B. Define assay and explain how samples are managed step by step with a clear explanation of each step. C. What type of data is being generated by this experiment?
- Answer the following questions. 1. Explain the steps associated with DNA profiling and state its advantages and disadvantages, 2. Describe how mutations are linked to DNA polymorphism. GUIDELINES: Each answer should be 250 words in length. Content should not be copied. References and bibliography should be provided for the content and images. please asapWhich of the following statements is/are FALSE about Bradford assay? 1. Upon addition of the reagent, the color of the protein solution becomes purple. II. The absorbance of the resulting solution is measured at a wavelength of 540-550 nm. Both I and II O II only Neither I nor II O I onlyExplain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to address the role of Taq DNA polymerase.