You are studying the DNA binding protein CLAMP and you want to determine its binding affinity for different DNA constructs, what technique should you use? ELISA PCR Chromatography Western Blotting Fluorescence polarization

Why amplify DNA using PCR before doing electrophoresis? Why not just take your DNA sample and directly run a gel?

The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)

describe the two main parts of a plasmid DNA extraction and purification procedure including the purpose of each step