For the nitrate reduction test, complete the table below Reaction Gas evolution (Yes/No) Color after adding reagents Color after adding zinc dust NO3 to NO2 NO3 to N2 NO3 to ammonia No reaction
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For the nitrate reduction test, complete the table below
|
Reaction |
Gas evolution (Yes/No) |
Color after adding reagents |
Color after adding zinc dust |
|
NO3 to NO2 |
|
|
|
|
NO3 to N2 |
|
|
|
|
NO3 to ammonia |
|
|
|
|
No reaction |
|
|
|
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- A solution is tested with three indicators and it has a pH of 9.8. Identify the colours of the following indicators: alizarin yellow R color:yellow,orange or red thymol blue: yellow,orange,blue or red bromothymol blue: yellow, green or blueWhich of the following is FALSE? Question 3 options: A Some bacteria reduce nitrate to nitrite B Some bacteria reduce nitrite to ammonia and/ or nitrogen gas C Sukfanilic acid and alpha- naphthylamine are used to detect if nitrites are present D A clear result after adding reagent A and B is negative for nitrate reduction E A clear result after adding zinc is a positive result for nitrate reductionMost of the following conclusions regarding the photo shown above are correct Except: All of the following are correct regarding the disk diffusion test photos EXCEPT : Zone of inhibition Chlorine Chlorine Chlorine O-phenylphenol Hexachlorophene O-phenylphenol Hexachlorophene Hexachlorophene O-phenylpheno Quat Quat Quat Staphylococcus aureus (gram-positive) Escherichia coli Pseudomonas aeruginosa (gram-negative) (gram-negative) Select one: O to. Gram negative bacteria are the most sensitive to chemical agents O b. Only one of the four chemicals affected Pseudomonas O c. Gram positive bacteria are the most sensitive to disinfectants O d. Hexachlorophene was effective only against Gram positive bacteria. O and. Chlorine was effective against all bacteria bacteria tested
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- Nitrate reduction test can be used to differentiate between Pseudomonads and Enterobacteriaceae. You inoculated your Nitrate broth with your unknown; after incubation, you added 5 drops of reagent A and 5 drops of reagent B. You observe a red color within 2 minutes. Is this a + or a - reaction? Assuming you did not get a red color, you then added a pinch of Zinc and this time tou obtained a red color. What does this mean?All recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…All recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…