Which of the following is FALSE? Question 3 options: A Some bacteria reduce nitrate to nitrite B Some bacteria reduce nitrite to ammonia and/ or nitrogen gas C Sukfanilic acid and alpha- naphthylamine are used to detect if nitrites are present D A clear result after adding reagent A and B is negative for nitrate reduction E A clear result after adding zinc is a positive result for nitrate reduction

Which of the following is FALSE? Question 3 options: A Some bacteria reduce nitrate to nitrite B Some bacteria reduce nitrite to ammonia and/ or nitrogen gas C Sukfanilic acid and alpha- naphthylamine are used to detect if nitrites are present D A clear result after adding reagent A and B is negative for nitrate reduction E A clear result after adding zinc is a positive result for nitrate reduction

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 2.4P

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Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive test
Which of the following is TRUE about the Nitrate Reduction test? Question 1 options: A Nitrate oxidase is the enzyme present for a positive test result B Pseudomonas aerugenosa can use oxygen instead of NO2 for aerobic respiration C Ammonia is reduced to NO2 by some bacteria D Oxygen is needed to reduce nitrate E It tests the ability to reduce NO3 to NO2 or ammonia or nitrogen
Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible
Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.
The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolism
The number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar by the pour plate method. The plates were inoculated aerobically for 48 hours at 37°C. What can you conclude from this data? Why? Mouthwash CFU per ml in salvia Before Use After Use Listerine 2.22 x 107 1.22x107 Schmidts Mouthwash 2.42 x 107 3.22x105 Biotene Mouthwash 3.21 x 107 2.12 x106
Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.
Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?
A milk sample was tested for the presence of typhimurium. Using a 10 microliter calibrated loop, a sample was taken directly from the milk and plated onto nutrient agar and the following day after incubation 98 colonies were counted. What is the CFU/ml of the S. typhimurium in this sample?
Serial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: In a tabulated form, determine if the Pseudomonas aeruginosa, are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: Answer the following In a tabulated form, determine if the Salmonella typhi, are positive or negative withcitrate test. Interpret your answer and Include the reaction of organism on this test.

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A	Some bacteria reduce nitrate to nitrite
B	Some bacteria reduce nitrite to ammonia and/ or nitrogen gas
C	Sukfanilic acid and alpha- naphthylamine are used to detect if nitrites are present
D	A clear result after adding reagent A and B is negative for nitrate reduction
E	A clear result after adding zinc is a positive result for nitrate reduction