Q: In pcr experiment, Does electrophoresis show that only DNA products of the desired size are present?…
A: A DNA sequence may be amplified trillions of times via PCR, yielding enough DNA copies for further…
Q: What is linker scanning mutagenesis?
A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…
Q: How can the concept of recombination frequency be used in genetic mapping?
A: It is the process of determining the location of genes on a chromosome that is called genetic…
Q: What is central dogma of molecular genetics? How does it work ?
A: The Central Dogma was first proposed in 1958 by Francis Crick. He was also the discoverer of the…
Q: What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that…
A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…
Q: If a pair of 10-mer primers are used in a PCR, what would be the number of expected binding sites in…
A: PCR is a polymerase chain reaction, in this process DNA is amplified . For this amplification,…
Q: List three rules for designing primers?
A: A primer is short ,single strand DNA sequence used in the polymerase chain reaction. Primers are…
Q: Differentiate between Sanger sequencing and next generation sequencing?
A: The process of determining the nucleic acid sequence is known as DNA sequencing.
Q: A method for detecting methylated CpGs involvesthe use of a chemical called bisulfite, which…
A: Hi! Since you have posted multiple questions, we are answering only first three sub-parts. Primers…
Q: In which of the following is DNA soluble or more soluble Water Ethanol or ethyl alcohol
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: Why is DNA supercoiling called supercoiling rather than just coiling? Why is positive supercoiling…
A: DNA supercoiling is an important process for the cells as it allows the packings of large DNA into a…
Q: What is meant by “proofreading” with respect to DNA polymerase?
A: DNA polymerase belongs to the member of the family of enzymes that play the role in catalyzing the…
Q: What are the benefits of using a mixture classification scheme as outlined in DNA Box 14.1? What…
A: INTRODUCTION German stain commission This commission includes several forensic science and legal…
Q: (a) Why can there be multiple codons for an amino acid? Why would this have evolved? (b) What is…
A: The genetic code comprises the set of rules that tells how the four nucleotide bases in the DNA are…
Q: Describe step 1: hybridization of template and primer in automated sanger sequencing
A: Introduction Sanger sequencing is also known as the “chain termination method”. It is a method for…
Q: When applying a genetic algorithm,when are the strings of binary digits often used?
A: Genetic algorithm is defined as the algorithm that reflects the natural selection process in which…
Q: Please describe the Watson-Crick proposal.
A:
Q: What happens when the calf liver DNA preparation is digested with EcoRI and what does this tell you…
A: Hi, Thanks For Your Question. Answer : When DNA, In This Case Calf Liver DNA, Is Digested With…
Q: What is the most convenient way of understanding a testcross problem in genetics?
A: In genetics, test cross is the breeding of an individual with a phenotypically recessive individual,…
Q: Design forward and reverse primers to amplify this ENTIRE locus by PCR. Design each primer to be 10…
A: The forward and reverse primers are both important components used in PCR for amplifying a target…
Q: What does whole exome sequencing test for?
A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…
Q: In modern molecular genetics, what is recombinant DNA, and how is it prepared?
A: Deoxyribonucleic acid (DNA) is the genetic material that carries genetic information in the form of…
Q: What primers will be used to sequence your PCR product where are those sequences on your PCR product…
A: PCR is a technique used for amplification of DNA/gene of interest. At the end of the PCR technique…
Q: In the procedure for isolating DNA, what is the purpose of adding washing-up liquid? Washing up…
A: DNA extraction is a popular technique used for isolating DNA from other cellular contents by…
Q: process of overlap assembly in whole genome sequencing
A: Genome sequencing Genome sequencing or gene sequencing is a way of arranging nucleotides the n the…
Q: Identical twin brothers begin life with identical genomes andepigenomes. How will this circumstance…
A: Identical twins share same genome and epigenome as they are born from same zygote, or it can be said…
Q: Define about Sanger sequencing ?
A: Introduction:- The process of determining the sequence of nucleotide bases (As, Ts, Cs, and Gs) in a…
Q: Why does evenly distributed peak in DNA chromatogram is an indication of a good sequence?
A: Evenly spaced peaks indeed indicate a good DNA sequence. Generally, four colours of peaks are…
Q: Give a step by step method and diagram of how reversible terminator sequencing works.
A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…
Q: If you ran the amplicon from the previous question (so it's about 3.7k basepairs long), on a gel,…
A: Agarose gel electrophoresis is a visualization tool that separates DNA molecules, based on their…
Q: When is low diversity especially problematic in sequencing?
A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…
Q: why single-nucleotide polymorphisms (SNPs) are important ?
A: DNA is a double standard molecule made up of nucleotides. Each nucleotide is made up of deoxyribose…
Q: Gel Electrophoresis Separates DNAFragments According to which thing?
A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…
Q: Describe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is…
A: Step 1 Sequencing is the establishment of the primary structure of an unbranched polymer. Sequencing…
Q: What is inverse PCR? How are we going to use inverse PCR to help figure out the molecular location…
A: The gene is a basic physical and functional unit of heredity. Genes are made up of DNA. Some genes…
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A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: Would you be more likely to find single nucleotidepolymorphisms (SNPs) in the protein-coding or in…
A: Single nucleotide polymorphisms in short SNPs refer to the variation in a single base pair that…
Q: Assume 2x108 reads of 75 bps long are obtained from a next-generation sequencing experiment to…
A: Next-generation sequencing relies on the continuous sequencing of the genome parts and later…
Q: Explain how Sanger sequencing works
A: Genome sequencing: genome means total gene content present in the organism and sequence means to…
Q: What is a short tandem repeat (STR) and which method do we use to detect it and which part of the…
A: Short-term repetition of tandem (STRs) is a short-lived sequence of DNA (2–6 bp) that accounts for…
Q: How accurate is whole exome sequencing?
A: The goal of exome sequencing, also known as whole genome sequencing is to identify genetic variants,…
Q: What is a processive enzyme? Explain why processivity is an important feature of DNA polymerase.
A: Enzymes are substances produced by living organisms to carry out reactions at a high rate without…
Q: 5'-ATGTCCACTGCGGTCCTGGAAAACCCAGGC-3’ wild-type allele 3'-TACAGGTGACGCCAGGACCTTTTGGGTCCG-5’…
A: The term mutation refers to the alternations in DNA sequences. The chemicals or other substances…
Q: In the procedure for isolating DNA, what is the purpose of adding washing-up liquid? Washing up…
A: By disrupting the nonpolar connections that keep the cell membrane together, it allows the cell…
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Q: What it is called when two regions of the genome in different species share similarities like those…
A: Genome Genome can be defined as the the complete set of of gene of an living organism.
Q: If you have access to the necessary computer software, make asequence file and analyze it in the…
A: The sequencing of genetic information is important for proper study and analysis, and with the…
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- What are the consequences for a DNA sequencing reaction if the ratio of dideoxyribonucleoside triphosphates to deoxyribonucleoside triphosphates is increased? What happens if this ratio is decreased?Assume 2x108 reads of 75 bps long are obtained from a next-generation sequencing experiment to sequence a human genome. Suppose the length of the human genome is 3x109 bps. What is the depth (i.e., coverage) of the sequencing?What is recombination? Mention its applications with reference to genetic engineering.
- What is a read (Sanger sequencing)?You are using the restriction enzyme HAEIII to digest different samples of the taster gene isolated from cheek cells of different people and amplified by PCR. When viewing the bands on the electrophoresis gel, one would expect that a taster (homozygote) would have---------band(s), whereas a carrier (heterozygote) would show--------band(s), and a non-taster would show------band(s).Describe the possible outcome of a PCR experiment in which (a) there is a single-stranded break in the target DNA sequence, which is present in only one copy in the starting sample, and (b) there is a doublestranded break in the target DNA sequence, which is present in only one copy in the starting sample.
- For the following short sequence of double stranded DNA and the given primers, there will be one major duplex DNA product after many cycles (imagine 10 cycles) of PCR. Provide the sequence of this one major duplex product and label the 5’ and 3’ ends of each strand. Sequence to be amplified: 5’- GGTATTGGCTACTTACTGGCATCG- 3’ 3’- CCATAACCGATGAATGACCGTAGC- 5’ Primers: 5’-TGGC-3’ and 5’-TGCC-3’What is the primary disadvantage of Sanger sequencing?In Figure , what do the red and blue parts of the DNA labeled by balloon 6 represent?
- Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers are present or (b) an excess of primers is present.What is the principle of Sanger sequencing?Pedigree Analysis Is a Basic Method in Human Genetic: What does OMIM stand for? What kinds of information are in this database?