What is a short tandem repeat (STR) and which method do we use to detect it and which part of the DNA are they in?

Q: What are the interesting characteristics of DNA quantitation? What are the positive and negative…

A: DNA quantification is done to know it's concentration and purity of the sample. This can be done…

Q: What is being pointed by the green arrow and what is its role on the DNA?

A: Nucleic acids are present inside the nucleus to store and pass genetic information and thus controls…

Q: What is the name of the DNA duplication process? What is the main enzyme that participates in it?

A: Every living cell divides to form multiple cells. During this division process, there is another…

Q: How is the melting curve of duplex DNA aff ected by adding a small amount of ethanol?

A: Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA…

Q: If the length of E.coli DNA is 1.36 mm,can you calculate the number of base pairs in ECOLI?

A: DNA is the genetic material in E.coli. It has a circular DNA molecule 4.6 million base pairs in…

Q: What causes different linear DNA molecules to migrate at different speeds during electrophoresis?

A: In molecular biology many techniques are used for different purpose like PCR is used to generate…

Q: How does size of DNA affect size traveled during electrophoresis?

A: Electrophoresis is a technique for the separation followed by the analysis of the macromolecules…

Q: Why do we need to amplify the DNA at all in order to visualize it in a gel?

A: PCR (polymerase chain reaction ) is a widely used technique to amplify DNA while Gel electrophoresis…

Q: During dna electrophoresis what fragments migrate towards? Larve or small!  Which charge slower…

A: Introduction :- Electrophoresis is a bio-physical technique , that is being used to separate the…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: Which nucleotides break the rules of Watson–Crick basepairing when they are found at the wobble…

A: The wobble hypothesis states that the base at 5' end of the anticodon is not spatially confined as…

Q: How does gel electrophoresis separate different-sized DNA fragments?

A: Gel electrophoresis is a molecular biology technique used to separate macromolecules like DNA, RNA…

Q: What is a repetitive element in genomics?  What are the types of repetitive elements?  What is their…

A: The amount  of DNA present in a cell of a person is called as genome. DNA is made up of nucleotides…

Q: What is the role of NaCl in the DNA isolation procedure?

A: NaCl (Sodium Chloride) is used during various DNA isolation procedures. Usually, in DNA isolation,…

Q: What different methods were used to help break the genetic code? What did each method reveal and…

A: The genetic code is a triplet code, in which three nucleotides encode each amino acid in a protein.…

Q: DNase is an endonuclease that degrades DNA. Which steps or/and reagents in the DNA isolation…

A: Restriction enzymes can be divided into two types viz., restriction endonuclease and restriction…

Q: In the procedure called RNA sequencing (RNA-Seq), what type ofmolecule is actually sequenced?

A: Ribonucleic acid sequencing (RNA-seq) was developed by Michael Snyder and colleagues in 2008. It is…

Q: what kinds of techniques are used to sequence large fragments of dna upto 50kb

A: With the advancement of science and technology in the field of life sciences, there has emerged…

Q: Why are the proteins cleaved into small fragments for protein sequencing?

A: Proteins are the end product of the molecular translation that codes the information present in the…

Q: When a segment of DNA containing either aVNTR or an RFLP is analyzed, the result is fragments of DNA…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: What additive in the agarose gel solution allows the DNA to fluoresce (glow) under UV light? How…

A: Electrophoresis is the migration of charged particles under the influence of an electric current.…

Q: What are Watson-Crick base pairs? Why are they important?

A: DNA or deoxyribonucleic acid is a type of biomolecule. It is a polymer of deoxyribonucleotides…

Q: What is the function of the chaotropic salt in DNA isolation?

A: These salts are strong protein denaturing agents.

Q: How many different reading frames are possible in a stretch of double-stranded DNA?

A: Introduction -open reading frame = • In molecular genetics, an open reading frame (ORF) is the part…

Q: Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the…

A: DNA is extracted from various sources to study the sequence of the DNAs from various sources and to…

Q: What are the major differences between dna probe and Primer

A: DNA as a genetic material in all the living organisms.

Q: What are some difficulties with low copy number DNA analysis? What kinds of checks and balances can…

A: INTRODUCTION DNA analysis DNA analysis is a method of analyzing the characteristics of DNA. It is…

Q: What are the barriers to getting DNA out of a cell (in particular a plant cell)?

A: Cell is a structural and functional unit of living organisms. Several cells joined together to form…

Q: What is a DNA library? Do you think this name is appropriate?

A: In terms of molecular biology, a library can be defined as the collection of DNA fragments that are…

Q: can recombine their genome with foreign DNA. What are the methods bacteria use to recombine their…

A: Like  eukaryotic cells, prokaryotic cells also show gene transfer and this process is called genetic…

Q: What is the role of a primer in DNA replication? What would happen if you forgot to add a primer in…

A: DNA replication is the process in which a parent DNA is separated and two new strands are formed…

Q: Draw the following structures and rate their relative solubilities in water (most soluble to least…

A: DNA is one of the types of nucleic acid. This structure is made up of small units called…

Q: What is the function of the alcohol in the extraction of DNA? What is the property/characteristics…

A: DNA extraction involves different steps which include, the breaking of cells to open and…

Q: The goal of many computer programs is to identify sequenceelements within a long segment of DNA.…

A: The collection of instructions executed to perform a specific task by a computer is called the…