Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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What is a short tandem repeat (STR) and which method do we use to detect it and which part of the DNA are they in?
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- Give typing answer with explanation and conclusion to all parts Maxim-Gilbert and Sanger Sequencing are two different methods used to sequence DNA. Describe the general techniques of Maxim-Gilbert and Sanger DNA Sequencing. List the advantages and disadvantages of each.arrow_forwardWhat are cis acting DNA sequences? List themarrow_forwardTranscriptome analysis involves two separate methodologies: gene expression and RNA seq analyses. The 10 items below are a scrambled listing of the steps used in the two procedures. Identify the steps involved in RNA seq from the list below. Use the numbers in the list to refer to each step. Once the steps for RNA seq have been identified, write the steps in the order in which they are performed during the experiment. (1) DNA sequencing (2) Allow for hybridization and wash excess cRNA. (3) Mix labeled cRNA with array chip. (4) PCR amplification (5) Measure fluorescence intensity to determine abundance of transcripts. (6) Add labeled cRNA at each microarray location. (7) Map cDNA sequences to the genome of the organism to determine identity and abundance of transcripts. (8) mRNA isolation from cells (9) Prepare fluorescently labeled cRNA probes (10) cDNA synthesisarrow_forward
- The goal of many computer programs is to identify sequence elementswithin a long segment of DNA. What is a sequence element? Givetwo examples. How is the specific sequence of a sequence elementdetermined? In other words, is it determined by the computer programor by genetic studies? Explain.arrow_forwardWe isolate DNA from a bacteria that is wild type for the genes arg leu and his shear it in a blender mix it into a culture of bacteria that is mutant for the genes arg leu and his . We then plate on various media. These are the results: Media with arginine 20 colonies /ml Media with histidine 300 colonies /ml Media with leucine 500 colonies /ml Media with all three 10000 colonies/ml 21. What is this experiment called? A. transpiration B. conjugation C. transformation D. transductionarrow_forwardIn the protocol for extracting DNA, what is the purpose of the clear shampoo in the DNA extraction buffer? In the protocol for extracting DNA, what is the purpose of the clear shampoo in the DNA extraction buffer? Shampoo contains detergents, that help to break down lipids in cell walls, thus freeing the DNA. Shampoo contains detergents, that help to break down proteins in cell membranes, including the nuclear membrane, thus freeing the DNA. Shampoo contains salts, that help to break down lipids and proteins in cell membranes, including the nuclear membrane, thus freeing the DNA. Shampoo contains detergents, that help to break down lipids in cell membranes, including the nuclear membrane, thus freeing the DNA.arrow_forward
- What assay uses the fact that activators can be separated into DNA binding and activating domains? What information do you gain from this assay? Describe briefly how to perform this assay.arrow_forwardHow are DNA molecules visualized in a gel after electrophoresis? Why do DNA molecules migrate toward the + electrode? What determines the rate of their migration? What is the effect of PEG on DNA fragments of different sizes? How is this influenced by the concentration of PEG?arrow_forwardIf you have access to the necessary computer software, make asequence file and analyze it in the following ways: What is thetranslated sequence in all three reading frames? What is the longest open reading frame? Is the sequence homologous to any known sequences? If so, does this provide any clues about the function of the sequence?arrow_forward
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