What is a short tandem repeat (STR) and which method do we use to detect it and which part of the DNA are they in?

Q: What is being pointed by the green arrow and what is its role on the DNA?

A: Nucleic acids are present inside the nucleus to store and pass genetic information and thus controls…

Q: Your company has decided to develop a PCR test that will be used to determine Huntington's disease.…

A: The polymerase chain reaction (PCR), is one of the most useful molecular biology techniques used to…

Q: How is the melting curve of duplex DNA aff ected by adding a small amount of ethanol?

A: Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA…

Q: Why do we need to amplify the DNA at all in order to visualize it in a gel?

A: PCR (polymerase chain reaction ) is a widely used technique to amplify DNA while Gel electrophoresis…

Q: Your graduate advisor asks you to amplify the following sequence of DNA by PCR:…

A: The primer should be complementary to the region of template to be amplified.

Q: What is the role of Mg2+ in genomic DNA extraction?

A: DNA extraction refers to the method of isolating and purifying DNA from a cell. It involves…

Q: Restriction endonuclease digestion of a DNA sequence yielded fragments of the following sizes: 1.…

A: The objective of the question is to determine the order of DNA fragments after gel electrophoresis…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: What is a Watson–Crick base pair?

A: Two nucleotides in the complementary strands of the nucleic acids are connected to each other by…

Q: What is meant by a primer, and why are primers necessary for DNA replication?

A: Primers are short DNA or RNA sequences complementary to the existing DNA strands. Without a primer a…

Q: What is Simple sequence repeat?

A: The genome of every individual contains millions of genetic variation or variants which make each…

Q: A new technique for rapidly determining the nuclesome structure for regions of chromatin is called…

A: In the field of molecular biology, understanding the mechanisms underlying gene expression…

Q: Which nucleotides break the rules of Watson–Crick basepairing when they are found at the wobble…

A: The wobble hypothesis states that the base at 5' end of the anticodon is not spatially confined as…

Q: What is recombination? Mention its applications with reference to genetic engineering.

A: Answer: Introduction: Recombination means the method of generating a new blend of genes by crossing…

Q: The telomeres of a linear chromosome are made of identical directly repeated DNA sequences. How does…

A: Telomeres:These are the special DNA caps in the eukaryotic chromosomes that prevent the loss of…

Q: You are asked to sequence a piece of DNA to determine if it is from a gene thought to be involved in…

A: DNA sequencing is an important technique used to determine the sequence of unknown fragments of DNA…

Q: Why is the term “proteome” ambiguous, whereas the term“genome” is not?

A: The proteins are the essential part of living organisms and it consists of thousands of smaller…

Q: Ailee is interested to determine the nucleotide sequence of her bacterial heat shock gene. Hence,…

A: In response to temperature changes, the heat-shock response involves the induction of many proteins…

Q: A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently…

A: The peptide sequence YSPTSPS is a known phosphorylation site that corresponds to the heptad repeat…

Q: Examine the DNA sequence shown below. You have been tasked with designing Primers for PCR…

A: Polymerase chain reaction (PCR) is a method used to  make millions of copies of a specific DNA…

Q: What is sheared DNA?

A: DNA is a double helical structure that serves to store the genetic information. It is a long…

Q: A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2%…

A: During agarose gel electrophoresis, an agarose solution is prepared, it is cooled to prepare a gel,…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: When a segment of DNA containing either aVNTR or an RFLP is analyzed, the result is fragments of DNA…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: What is the hybridization and synthesis process and why it is needed?

A: In the cell, there are several processes, which are required for survival and biochemical functions.…

Q: Where would you expect DNA-binding proteins to bind if they recognize a specific base sequence? What…

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that transfers genetic information from one…

Q: What additive in the agarose gel solution allows the DNA to fluoresce (glow) under UV light? How…

A: Electrophoresis is the migration of charged particles under the influence of an electric current.…

Q: A piece of DNA 5.0 kb long is cloned and then cut out of the vector for analysis. This linear piece…

A: Restriction enzyme is any enzyme that cleaves DNA at a particular sequence. Example - BamHI, EcoRI,…

Q: What are Watson-Crick base pairs? Why are they important?

A: DNA or deoxyribonucleic acid is a type of biomolecule. It is a polymer of deoxyribonucleotides…

Q: In the procedure for isolating DNA, what is the purpose of adding washing-up liquid? Washing up…

A: DNA extraction is a popular technique used for isolating DNA from other cellular contents by…

Q: How would you solve this and how would it look? i am confused

A: The objective of this question is to estimate the size of an unknown DNA fragment using a standard…

Q: What is meant by a primer, and why are primers necessary for DNA replication?

A: DNA replication produces new DNA molecules using the existing DNA strand as a template.

Q: Why do DNA chips often contain segments derived from cDNA rather than genomic DNA segments?

A: DNA chip used in microarray

Q: In producing genetically engineered human insulin in bacteria, why is it important to use the…

A: Insulin is a natural peptide hormone produced by the beta cells of the pancreas in response to high…

Q: How many different reading frames are possible in a stretch of double-stranded DNA?

A: Introduction -open reading frame = • In molecular genetics, an open reading frame (ORF) is the part…

Q: Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers are…

A: Chain terminating sequencing is also known as Sanger sequencing which is based on amplification of…

Q: Why does evenly distributed peak in DNA chromatogram is an indication of a good sequence?

A: Evenly spaced peaks indeed indicate a good DNA sequence. Generally, four colours of peaks are…