What is a processive enzyme? Explain why processivity is an important feature of DNA polymerase.

Q: Why does heat denature, or melt, DNA in solution?

A: DNA or deoxyribonucleic acid is the genetic material of most of the organisms except RNA viruses. It…

Q: What are the interesting characteristics of DNA quantitation? What are the positive and negative…

A: DNA quantification is done to know it's concentration and purity of the sample. This can be done…

Q: What do you mean by DNA polymerase δ?

A: Introduction: DNA polymerase δ is one of the multiple types of DNA polymerases found in eukaryotes.

Q: what are some examples where DNA polymerase is not functional and translesion DNA synthesis has to…

A: Dna polymerase are the enzyme that helps in DNA synthesis. It has the ability to synthesize the DNA…

Q: Does Phospodiester bonds hold deoxyribose and phosphate components together in the DNA

A: Ans- true

Q: What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that…

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…

Q: In a Sanger DNA sequencing reaction (dideoxy method) you use the primer 5′-GCATATA-3′ to sequence a…

A: Sequencing of DNA refers to the biochemical methods for determining the order of the nucleotide…

Q: In gel electrophoresis, are the DNA fragments attracted to, or repelled from the negative pole of…

A: Electrophoresis is a process where charged particles move under the influence of the electric field.…

Q: What is the importance of the 3' to5' exonucleasevactivity of DNA polymerase

A: Enzymes serve as biological catalysts that fasten the rate of reaction by lowering the activation…

Q: What is meant by a primer, and why are primers necessary for DNA replication?

A: Primers are short DNA or RNA sequences complementary to the existing DNA strands. Without a primer a…

Q: The topoisomerase enzyme catalyzes the interconversion of the relaxed and supercoiled forms of DNA…

A: DNA replication is the process of the formation of identical copies of DNA. It occurs in nucleus of…

Q: Ethanol promotes bonding between Na+ ions from the salt and charged phosphate group of the DNA due…

A: DNA extraction is a molecular process that involves extraction of DNA from biological samples. It…

Q: What is meant by “proofreading” with respect to DNA polymerase?

A: DNA polymerase belongs to the member of the family of enzymes that play the role in catalyzing the…

Q: What is experiment of Thermodynamics of Small Oligomeric Duplex DNA Denaturation.

A: Double-stranded DNA is the most common kind of DNA molecule found in nature. The strands can be…

Q: explain the laws of DNA polymerase? Why are they important and are all the laws required for DNA…

A: DNA polymerase is the enzyme that is helpful in catalysing the DNA molecules synthesis. There are…

Q: What does DNA polymerase III need to catalyze DNA synthesis

A:

Q: What is the role of alcohol in extracting DNA?

A: DNA (Deoxy Ribonucelic Acid) extraction is carried out to perform experiments. The first…

Q: Explain the process of DNA elongation. In your discussion, make sure you discuss the direction of…

A: Basically, elongation is the stage when the RNA strand gets longer, thanks to the addition of new…

Q: A temperature-sensitive mutation is one in which the defect is not presented functionally until the…

A: Introduction  Enzymes are the biomolecules especially proteins. Which helps in the speeding up of…

Q: What is the purpose of adding table salt (NaCl) to the DNA extraction buffer? Table salt dissociates…

A: DNA is a double-helical structure whose backbone is made up of sugar-phosphate and bases are present…

Q: Assume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single…

A: The objective of the question is to calculate the number of copies of a single DNA fragment after…

Q: Why do DNA Polymerases Require a Primer?

A: DNA Polymerase DNA Polymerase is the enzyme responsible for normal DNA synthesis. Different kinds of…

Q: What are the requirements for strand elongation by DNA polymerase enzymes?

A: DNA replication in eukaryotes occurs in three stages: initiation, elongation, and termination,…

Q: Explain why a DNA polymerase that could synthesize DNA in the 3′ → 5′ direction would have a…

A: Introduction: The synthesis of DNA takes place in 5' to 3' direction together on the both strands of…

Q: In the procedure for isolating DNA, what is the purpose of adding washing-up liquid? Washing up…

A: DNA extraction is a popular technique used for isolating DNA from other cellular contents by…

Q: What is meant by “error-prone” DNA repair?

A: DNA replication is a process in which two DNA molecules are synthesized from a single DNA molecule.…

Q: DNA glycosylase inhibitors are used to study which DNA repair mechanism?

A: DNA glycosylase inhibitors are used to which DNA repair mechanism?

Q: An RNA-dependent DNA polymerase that carries the RNA template with it to synthesize repeats at the…

A: Replication is the process of duplication of the DNA. Replication is initiated by recognizing the…

Q: What is meant by a primer, and why are primers necessary for DNA replication?

A: DNA replication produces new DNA molecules using the existing DNA strand as a template.

Q: What are the purposes of the 3’-5’ and 5’-3’ exonuclease activities of DNA polymerase I and why they…

A: DNA polymerase is an enzyme which catalyze the synthesis of a complementary DNA strand from an…

Q: Can you explain what is nucleotide selectivity in polymerase

A: One of the structural elements, or building blocks, of DNA and RNA is the nucleotide. A nucleotide…

Q: Why does evenly distributed peak in DNA chromatogram is an indication of a good sequence?

A: Evenly spaced peaks indeed indicate a good DNA sequence. Generally, four colours of peaks are…

Q: enzymes ECORI and HINDIII are called "compatible" enzymes. DNA cut with these enzymes can be ligated…

A: Restriction Endonucleases Restriction endonucleases or restriction enzymes are the enzymes that can…