Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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What is a processive enzyme? Explain why processivity is an important feature of DNA polymerase.
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- DNA polymerase requires both a template, to be copied, and a primer, which provides a 3′ hydroxyl from which polymerase can extend. Yet this molecule supports DNA polymerase activity. Explain. pTGACACAGGTTTAGCCCATCGATGGG−OH Match the items in the left column to the appropriate blanks in the sentence on the right.arrow_forwardCan you help find the true statement of this problem?arrow_forwardDescribe a typical program used for DNA amplification with the thermal cycler and the purpose of each steps.arrow_forward
- The function of the 3'-> 5' exonuclease activity is found in which of the following: DNA Polymerase III DNA Polymerase I DNA Polymerase II All of the abovearrow_forwardWhy do longer DNA molecules move more slowlythan shorter ones during electrophoresis?arrow_forwardFollowing base removal, DNA polymerase can add nucleotides in the 5'-to-3' direction. Is that true or False? Why? please help me explain thatarrow_forward
- The Polymerase Chain Reaction is a molecular biology tool for exponentially amplifying DNA in a test tube in a cell free system. With this technique, DNA strands are separated by heating the mixture so that a DNA helicase is not required. A heat stable polymerase (Pol Z) is then used to copy DNA over and over in a tube. Recently an improved version of Pol Z was created by fusing Protein X, a DNA binding protein, to Pol Z (see left figure below). The right panel shows that fusion of Pol Z to Protein X significantly increases its efficiency. Briefly provide a plausible mechanism for how Protein X is improving Pol Z. Which prokarotic/eukaryotic protein activity is Protein X mimicking that increases the activity of the polymerase?arrow_forwardIn the protocol for extracting DNA, what is the purpose of the clear shampoo in the DNA extraction buffer? In the protocol for extracting DNA, what is the purpose of the clear shampoo in the DNA extraction buffer? Shampoo contains detergents, that help to break down lipids in cell walls, thus freeing the DNA. Shampoo contains detergents, that help to break down proteins in cell membranes, including the nuclear membrane, thus freeing the DNA. Shampoo contains salts, that help to break down lipids and proteins in cell membranes, including the nuclear membrane, thus freeing the DNA. Shampoo contains detergents, that help to break down lipids in cell membranes, including the nuclear membrane, thus freeing the DNA.arrow_forwardIn eukaryotes, what is meant by the term DNA replication licensing? How does the process occur?arrow_forward
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