For each of the coupling steps, you will add 4 times excess of the amino acid and 4 times excess of the coupling agent, HBTU. Using the following molecular weights of different Fmoc- proctected amino acids and HBTU, calculate how much of each amino acid or HBTUmyou will need to weigh out to have 4 equivalents (i.e. how much amino acid do you need to weigh out to get 0.4 mmol). Q5.1 If Fmoc-Thr(OtBu)-OH has a molecular weight of 397.47 g/mol, how much do you need to weigh out for 0.4 mmol?

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You will weight out 200 mg of resin. The resin already has the P1 lysine attached to it and has
an equivalence of 0.5 mmol/g, meaning if you used a gram of resin, you would be able to
synthesize 0.5 mmol of peptide (assuming 100% yield). However, with only 200 mg of resin, the
maximum about of peptide you can synthesize is 0.1 mmol.
For each of the coupling steps, you will add 4 times excess of the amino acid and 4 times
excess of the coupling agent, HBTU. Using the following molecular weights of different Fmoc-
proctected amino acids and HBTU, calculate how much of each amino acid or HBTUmyou will
need to weigh out to have 4 equivalents (i.e. how much amino acid do you need to weigh out to
get 0.4 mmol).
Q5.1
If Fmoc-Thr(OtBu)-OH has a molecular weight of 397.47 g/mol, how much do you need to weigh
out for 0.4 mmol?
Transcribed Image Text:You will weight out 200 mg of resin. The resin already has the P1 lysine attached to it and has an equivalence of 0.5 mmol/g, meaning if you used a gram of resin, you would be able to synthesize 0.5 mmol of peptide (assuming 100% yield). However, with only 200 mg of resin, the maximum about of peptide you can synthesize is 0.1 mmol. For each of the coupling steps, you will add 4 times excess of the amino acid and 4 times excess of the coupling agent, HBTU. Using the following molecular weights of different Fmoc- proctected amino acids and HBTU, calculate how much of each amino acid or HBTUmyou will need to weigh out to have 4 equivalents (i.e. how much amino acid do you need to weigh out to get 0.4 mmol). Q5.1 If Fmoc-Thr(OtBu)-OH has a molecular weight of 397.47 g/mol, how much do you need to weigh out for 0.4 mmol?
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