Q: What is the usual stain used for staining a sputum smear?
A: Essentially, sputum comprises of a combination of saliva and mucus. additionally named as phlegm, It…
Q: what is the procedure and materials you need to stain Staphylococcus aureus?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium hat belongs to the Firmicutes and…
Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…
A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…
Q: Why is it important to limit the quantity of cells used to prepare a smear?
A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…
Q: In what instances would the pour plate method be more appropriate than the spread plat method?
A: Pour plate method is a technique that used to enumerate the number of viable cells in a sample.…
Q: Why does a streaking method used to inoculate plates result in isolated colonies?
A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…
Q: What is the difference between culture-based technique and culture-independent techniques based on…
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: Transillumination is used clinically for the detection of:
A: Transillumination is an examination that is done with the help of a strong light shining on a…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
A: The simple dye is used in the technique of simple staining to highlight only the specific structures…
Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…
Q: When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times…
A: The initial identification of the streptococcal and enterococcal species is based on their hemolytic…
Q: shape
A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…
Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?
A: Answer: Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…
Q: If you had cultures of B. subtilis and E. aerogenes and forgot to label them, which of the following…
A: Some bacteria produce indole by deamination of amino acid tryptophan and shows positive indole test…
Q: describe the agar-overlay technique
A: Biological techniques are processes or methods for trying to study living things. They include…
Q: Why is it important to use a sterilized loop between streaks when preparinga streakplate?
A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…
A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…
Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?
A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate…
A: The microorganisms can be differentiated from each other based on their growth characteristics,…
Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…
A: The acid-fast staining technique is a differential staining technique. The bacteria which get…
Q: If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many…
A: Quadrant streaking is a microbiological technique which is used for isolation of pure strain from…
Q: Is it important to have a standardized way of counting and reporting AFB seen on a direct sputum…
A: Acid-Fast Bacilli (AFB) is a bacterial species that causes tuberculosis in humans and testing of…
Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…
A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…
Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…
A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…
Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…
A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…
Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…
A: Preparation of culture media and inoculation are two of the very sensitive steps in any…
Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…
A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…
Q: Is there a relationship between Gram staining and sensitivity to various antibacterial compounds?…
A: Gram staining is a method to identify whether a bacteria is gram positive or gram negative based on…
Q: What are the advantages and disadvantages of using the Wet Mount technique?
A: As per bartleby guidelines, we are suppose to answer only one question kindly post other separately.…
Q: Staining bacterial capsule can not be accomplished by?
A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…
Q: Are TSA, MRS and MOX differential media? Why or why not?
A: In microbial biology, differential media refers to any growth media that comprises of certain…
Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…
A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…
A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: What things can be done during sample collection of a sick patient to help eliminate the culture of…
A: Normal flora is the organisms that are present in the body for normal functioning. The normal flora…
Q: It is expected that amoxicillin would be more effective against Gram-positive bacteria. Explain…
A: Amoxicillin is a beta-lactam antibiotic that functions by attaching itself to the membrane proteins…
Q: What are the advantages and disadvantages of using the Slide Culture technique?
A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: What advantage does the oil immersion objective have over the low power objective when doing…
A: Introduction:- Bacteria are prokaryotic and very small sized organism's . They can not be visualised…
Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…
A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…
Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…
A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…
Q: What staining method would you use to verify that the isolate is not producing survival structures…
A: Staining is the procedure that helps to visualize the minute organisms under a microscope using…
Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…
If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate the pathogen from the sample and why?
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- When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times with the loop?Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedwhat is the procedure and materials you need to stain Staphylococcus aureus?
- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?describe the agar-overlay technique
- Describe the process to make a 4-phase streak plate beginning with your first streak (i.e you have bacterial culture on your sterile loop and are about to start to streak your agar plate ).In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?Briefly, in your own words, describe the steps of the gram staining procedure.
- Describe the Gram staining procedureWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleWhy are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation?