Explain the concept of and apply the aseptic technique
Q: What type of medium is used to maintain and preserve specimens before clinical analysis? a.…
A: Microorganisms are small, unicellular, and microscopic organisms. Any organism capable of causing…
Q: Illustrate the step by step method of preparing culture media.
A: Introduction Culture medium:- It is a liquid or gel designed for specific mixtures of nutrients and…
Q: For each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E…
A: EnteroPluri-Test is a 12-sector system that consists of special culture media. which allows…
Q: Describe several moist heat methods and their chief applications for sterilization and disinfection.
A: Disinfection is reducing or eliminating the number of microorganisms from the surface of the…
Q: Compare 2 applications of autoclaving and pasteurization with regard to antimicrobial control .
A: Microbial control is a way of controlling the growth or killing of Microbes and is done by…
Q: explain in your own words an introduction to a lab about Control of Microbial Growth - Antimicrobic…
A:
Q: Name two ways that pure, mixed, and contaminated cultures aresimilar and two ways that they differ…
A: Microorganisms are isolated and cultured for various reasons. Culture is the observable growth that…
Q: ... E Topic: laboratory Instrument Which of the following are TRUE regarding Which of the following…
A: INTRODUCTION The answers on the topic laboratory instruments are given below.
Q: If lysozyme is added to a microbial culture. Predict what will be observed if the culture is…
A: Microorganisms are small organism that cannot be seen by naked eyes. They include bacteria, fungi,…
Q: The following media are used to grow bacteria for various purposes. Match the examples of media that…
A: MacConkey Agar: - Selective Growth Medium Malt Extract Agar:- Complex Medium
Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…
A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…
Q: What are the physical methods of microbial control
A: Control of microorganisms is fundamental to forestall the transmission of illnesses and disease,…
Q: In product testing, microbial growth is found.| Interpretation: Rationale:
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Compare and contrast antisepsis, disinfection, sanitization, and sterilization with regard to…
A: Microbes are the microscopic organisms that grow on different objects and surfaces in the…
Q: Explain the mechanism of action of several of the physical and chemical agents employed to control…
A: Microbes such as bacteria, viruses, worms may act as nuisance causing various diseases in plants,…
Q: Why is the membrane filter method preferred to the most probable numbermethod (MPN) for the…
A: Most probable number (MPN) is a method used to estimate the numbers of bacterial cells in a water…
Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…
A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…
Q: Provide at least two other examples of samples that are best prepared using Smear Preparation…
A: Smear preparation is a technique that is used by spreading a small quantity of sample on the slide…
Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…
A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…
Q: Differentiate the various types of culture media for the growth of microorganisms: synthetic,…
A: Culture media, also known as growth media, are specific mixtures of nutrients and other substances…
Q: Explain how antimicrobial agents A through D in the fol-lowing diagram compare in effectiveness…
A: Microorganisms are organisms that cannot be viewed by unaided eyes and require a microscope for…
Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…
A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…
Q: What are the factors which need to be considered in the selection of culture vessel?
A: Culture vessels:- Culture vessels provide a contamination barrier to protect the cultures from the…
Q: Explain the significance of aseptic culture technique in growing microorganism
A:
Q: Enumerate the various Types of Microbial Control, identify the Principles Involved and give 3…
A: Controlling microbial growth is a crucial factor in pharmaceutical, biotechnology, medicine and food…
Q: Name 2 indirect methods for measuring microbial growth and describe how each is performed. a. i.…
A: Two indirect methods for measuring microbial growth are : 1. Turbidimetric method 2. Standard plate…
Q: What are the difference in the movement of microorganisms under wet mount from hanging drop…
A: There are some plus point for both the techniques like the wet Mount and hanging drop method…
Q: Compare and contrast moist and dry heat methods of microbe control and give 2 examples of each.
A: The growth of microbes in an inanimate object can lead to the contamination of the particular…
Q: Show a concept map about the aseptic technique on Transfer from Broth to Broth Culture, Transfer…
A: Aseptic techniques or sterile techniques are procedures that ensure proper handling and conduction…
Q: Outline the different physical and chemical methods of sterilization. Briefly describe how each…
A: Sterilization is the process that eliminates all forms of life either by deactivating, killing, or…
Q: What is differential medium
A: Differential medium or indicative media contain compounds which support the growth of microbes and…
Q: Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri…
A: Following aseptic techniques is an important part while performing an experiment. Aseptic technique…
Q: Name six basic nutritional requirements supplied in all culture media.
A: The culture media refers to the gel or liquid prepared for the growth of microorganisms. The culture…
Q: Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.
A: Aspetic techniques that you should follow during inoculating a broth from a plate culture are:…
Q: When testing antimicrobial compounds on lawns of plated bacteria, the strongest germicide could…
A: Bacteria are prokaryotic unicellular organisms that are omnipresent in the environment. There are…
Q: Describe the five phases of a microbial growth curve observed when growing microbes are grown in…
A: The bacterial growth curve represents the number of live cells in a bacterial population over a…
Q: Define the following terms; streak plate method, and selective media. State the purpose and…
A: Plating is a microbiological method used to separate microorganisms found in a sample. It is used to…
Q: Define the following termonologies in brief : Focal Adhesion Biofilm Calcification and examples…
A: "Since you have asked multiple parts of the same question, we are eligible to answer only the first…
Q: Control of Microbial Growth A) Which would be the best process to use and why? B) Can you use…
A: A. In the control of microbial growth, Heating is the best method for controlling microbial growth,…
Q: Differentiate between defined and complex culture media
A: To differentiate: Chemically defined and complex culture media
Q: It may seem contradictory that lyophilization can be used to preservefoods yet also be used to keep…
A: Lyophilization is a stabilizing process in which the substance is subjected to freeze drying and the…
Q: What is the goal of aseptic techniques O To prevent contamination To allow contamination all of the…
A: Aseptic techniques are those techniques that are used in the field of medical. They are a kind of…
Q: Describe the concept behind sterilization by fi ltration, including examples of applications.
A: While working in a laboratory, aseptic conditions have to be maintained. This means that the lab has…
Q: Which of following statements is TRUE about antiseptics and disinfectants? A. Disc diffusion works…
A: The use of antiseptics and disinfectants is now unavoidable but what factors affect these processes…
Q: Describe the process of qualitative water test for coliform contamination. If shown EMB plates, be…
A: * Coliform bacteria are Bacteria and stains Gram negative and they can be motile or non motile…
Q: Considering microbial identification methods, to which category would the following method belong…
A: Introductin The existence of catalase, an enzyme that catalyses the release of oxygen from…
Q: Explain why desiccation and cold are not reliable methods of disinfectionbut how they can still be…
A: Sterilization is a technique that removes, kills, or deactivates all forms of life and other…
Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar…
A: A microorganism culture may be a check accustomed verify whether or not a bacterium or fungi are…
Q: Defi ne and diff erentiate among the major terms for microbial control, citing examples of each.
A: Microbes are small organisms that cannot be seen with naked eyes.
Explain the concept of and apply the aseptic technique
Explain hanging drop and wet mount preparations of microbial specimens
Step by step
Solved in 3 steps
- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.Explain the significance of aseptic culture technique in growing microorganisms.List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?
- Outline the different physical and chemical methods of sterilization. Briefly describe how each method control microbial growthGive an example of a European standard for evaluating efficacy of disinfectant against one type of microorganism and show it through a systematic diagram of the protocol.Discuss in detail how you would prepare this solution using any of the standard laboratory equiment listed in the table above
- Give the use and advantages of the following cultures for the examination ofcolony characteristics: a. Agar plateb. Agar slantc. Broth culturesMake a list of the different types of sterilization, including the method, the principle, the materials that can be sterilized, the required temperature and time, and the required temperature and time. Method Principle Apparatus Temp/Time MaterialsWhat appropriate method should be used in controlling microbial growth in each of the following; chicken serum, fresh goat milk, nutrient broth, paracetamol and inoculation loop?
- Identify and describe the 4 wet-heat methods that are used to control microbial growth.Describe the concept behind sterilization by filtration, includingexamples of applications.What are the difference in the movement of microorganisms under wet mount from hanging drop technique? Provide a descriptive explanation.