EVOLUTION CONNECTION Identify the evolutionarymechanisms that might account for the origin andpersistence of cell-to-cell signaling systems in prokaryotes

What is a second messenger? What are two second messengers that are involved in signaling pathways downstream of GPCRs and explain how they are produced? long answer: If you wanted to compare a cardiac muscle cell with a skeletal muscle, how could you use RNA sequencing to identify genes that are differentially expressed? In your answer, explain this technique and describe what type of information you can learn from it. If RNA sequencing led to the identification of two genes, gene X and gene Y, what are two experiments you could do to study the functions of these two genes?