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Based on the simulation, what do you think are the possible practical applications of chromatographic techniques that are relevant to your program?
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- SEE EXAMPLE IN THE IMAGE A mixture of 0.20M acetic acid and 0.30M sodium acetate is given. Calculate the pH of the medium if the pKa of the acetic acid is 4.76. Show your complete solution.A solution is tested with three indicators and it has a pH of 9.8. Identify the colours of the following indicators: alizarin yellow R color:yellow,orange or red thymol blue: yellow,orange,blue or red bromothymol blue: yellow, green or blueAll recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…
- If the following lipids are present: phosphatidylserine / phosphatidylcholine / sphingomyelin / triacylglycerol / and cholesterol ester are mixed and the mixture is separated by column technique. chromatography with silica gel in the column and then start Leach with a less polar solvent. The drought gradually increases the polarity of the less polar solvent. and gradually increasing the polarity of the solvent How are the lipids in the mixture in order to emerge from the column? along with explaining the reasons ..Please show the solution. 2.Which compounds will be separated by column chromatography in this lab? Check all that apply. Orange G Methylene BlueMethylene Blue Polymer of galactose that is stained blue Polymer of galactose that is stained blue Polymer of glucose that is stained blue
- Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NAOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the folloving test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0. 1ml Unknown 0.1ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2. After each addition of the working Bradford reagent, thoroughly mix the…The table below summarizes the results for the solubility test. Which solvent the lipid samples are miscible? PUT A CHECK Hexane Ethyl Acetate Water Methanol A B C Which solvent the lipid samples are immiscible? PUT A CHECK Hexane Ethyl Acetate Water Methanol A B CAll recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…
- Properties of water & meniscus Which properties of water contribute to the formation of the meniscus? Select all that apply. Check All That Apply high heat of vaporization high heat capacity cohesion adhesion density universal solventMETHOD: Touch different surfaces after that sanitize your hand with 70% alcohol then touch the surface of a clean piece of bread and place it in a bag. Label the bag “70% ROH” Question: What is the result and write your conclusionChlorophyll is the green pigment in the leaves of the plant, carotene imparts orange coloration while anthocyanin is known as the purple pigment. In one of their laboratory, a group was tasked to confirm the presence of these pigments on a certain leaf. After crushing several leaves, they soak it in isopropyl paper strip was dried and the group got the following measurements from the submerged tip of the paper strip: solvent font - 9.9cm, purple ink -8.6cm, yellowish orange ink - 6.4cm and green ink - 4.2 retention factor of each pigment. (d) Rank each pigment according to their solubility in the given solvent.