Suppose you received two cultures from a reactor biological: one containing a species of Gram negative bacteria and the other, a species of Archaea. Discuss four different ways to distinguish these cultures, without considering ribosomal RNA sequencing.
Q: The k of water is 1.86 kg °C/mol, approximately 4x the value of k,. If you attempted to freeze your ...
A: Freezing point depression is a colligative property and it is noticed in solution when solute molecu...
Q: The carbonic acid equilibrium is shown below. Exhalation of CO2 by the lungs causes this equilibrium...
A: Equilibrium shifts to the side where the concentration of substance is lowered so as to produce more...
Q: What amino acid is technically not an amino acid and which amino acid contains no chiral carbon atom...
A: Protein is an organic nitrogenous macromolecule that is essential for human health. In the human bod...
Q: Starting from formaldehyde, ammonia, hydrogen cyanide, carbon dioxide, ethene and water as needed, s...
A: 4.5 billion years ago our planate originate from the solar system. at 1st billion years, there is...
Q: Why are polyunsaturated omega-3 fatty acids considered as good for our health? Explain such phenomen...
A: Fatty acids are important components of lipids. Fatty acids are composed of hydrocarbon chain and a ...
Q: Compound A below is a key intermediate in the synthesis of keto-myo-inositol B. Suggest a synthetic ...
A: Here compound C is D-Glucose D-Glucose is converted into Glucose-6-Phosphate in presence of Hexokina...
Q: Functional groups and linkages assignment Complete the reactions below. Circle and identify all func...
A: The hydrocarbon compound is N-butylpropanamide which is reacting with water.
Q: Given the following question on the image identify the following:1. Total number of acetyl coA molec...
A: Maltose is a carbohydrate and it gets oxidized through glycolysis in cytoplasm and through the TCA c...
Q: Is this an L or a D amino acid? Explain.
A: Amino acids are the building blocks of proteins which are composed of amino group (NH3+), carboxyl g...
Q: QUESTION 5 A weak acid solution can act as a good buffer when its pH is way below its pKa O around i...
A:
Q: do archaebacteria have autotroph or heterotroph nutrients
A: Nutrients are the components of food like carbohydrates, fats, vitamins, proteins, etc. These nutrie...
Q: Why can't we store Vitamin C in our bodies?
A: Vitamin C- Ascorbic acid It is vital for tissue repair , collagen synthesis and it's deficiency- cau...
Q: All of the following are true of antigen-presenting cells (APCs) EXCEPT: which option below is the ...
A: Antigen-presenting cells (APCs) are the heterogeneous group of immune cells which can process antige...
Q: Based on our class discussion of the currently accepted mechanism for ribosome catalysis, draw a gra...
A: By transferring the nascent peptide chain from P-site peptidyl-tRNA to A-site aminoacyl-tRNA (aa-tRN...
Q: Describe how the following properties affect the function of a protien: A.) R group orientation B.) ...
A: Amino acids are biomolecules that are comprised of two functional groups, these are an amino group (...
Q: Why is biochemistry called the chemistry of life?
A: The question is all about the subject biochemistry that it is called as chemistry of life as our hum...
Q: MAKE A CONCLUSION OR SUMMARY how carbohydrate solutions (glucose, fructose, lactose, sucrose, and st...
A: All these tests mentioned are test to distinguish different type of sugar in given mixture or soluti...
Q: Which of the following has the highest iodine content? Which of the following has the lowest meltin...
A: 1. Linoleic acid has the highest iodine content. 2. Unsaturated fatty acids have lower melting poin...
Q: How ENDOSYMBIOTIC THEORY applies to BIOMOLECULES?
A: Endosymbiotic theory explains the phenomenon by which 2 previously independent structures undertakes...
Q: All of the following are true of the lymphatic system, EXCEPT: which option below is the answer? N...
A: Lymphatic system: It is a system of thin tubes that runs through the body called the lymph vessels....
Q: 3. A patient has got excess carbohydrate meal for the years and gain the weight. To explain this: a)...
A: A.) SCHEME OF TAG SYNTHESIS IN LIVER
Q: A solution has a hydrogen ion concentration of 0.01 mol/L. What is its pH? What is its hydroxide ion...
A: Given Values: [H+] = 0.01 Mol/L = 1×10-2 M pH is a measure of negative logarithm of hydrogen ion con...
Q: 1a-Membrane bound proteins often contain transmembrane domains. These transmembrane domains cont...
A: Membrane proteins can be of three major types- membrane bound/integral, peripheral and lipid anchore...
Q: Dexaclomycin is an antibiotic that inhibits peptidoglycan formation. Given this information, what ty...
A: Gram-negative bacteria are surrounded by a thin peptidoglycan cell wall, and an outer cell membrane ...
Q: is not formed during the Krebs cycle. *
A: The citric acid cycle is the metabolic pathway of the biomolecules that occurs in the matrix of mito...
Q: Some of the test(s) you tried from this virtual lab are important basis for determining glucose leve...
A: The sugar present in the blood or urine sample is glucose.
Q: 1. What organelles/parts of the cell are present in all types of cells, whether prokaryotes or eukar...
A: All living things are made up of cells, which are the structural, functional, and biological compone...
Q: Do glycolic acid and lactic acid belong to the same homologous series? Give a reason for your answer...
A: Homologous series is a series of chemical compounds having similar chemical properties and some of t...
Q: 1. Would you say that indicator tests are qualitative or quantitative? Explain your answer.
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any ...
Q: a. Identify the regulatory process an%3; b. Explain how this process works
A: Enzymes are biocatalyst. They act on a substrate and product is formed. Enzymes increases the speed...
Q: 1mL of the stock solution acetaminophen (1mg/mL) is used for the estimation of PC using n-octanol. A...
A: Acetaminophen is also commonly known as a Paracetamol that is a medicine used to treat the fever and...
Q: SDS-PAGE gels are useful in determining the molecular weights of proteins; however, the molecular we...
A: SDS-PAGE is an acronym for sodium dodecyl sulfate–polyacrylamide gel electrophoresis. It’s a mass-ba...
Q: For the following, calculate pka, and pka, of 1 Glycine, determine the 2 buffering regions, and calc...
A: Glycine is an amino acid with H-atom as the side chain. The isoelectric point of a amino acid (pI) i...
Q: 1. A ligand binds more tightly to the folded state (N) of a protein than to the unfolded state (U). ...
A: "Since you have asked multiple questions, we will solve the first question for you. If you want a sp...
Q: Do cultivated plants suffer from diseases just like humans?
A: All living beings have cells, which are the most basic fundamental unit of life. Cells are responsib...
Q: a. You selectively label phospholipids with a fluorescent dye and perform the FRAP assay. You detect...
A: FRAP is a method to determine the diffusion or kinetics of the biomolecule through tissue or cells. ...
Q: Regulation of glycolysis pathway involves. . Allosteric inhibition by ATP Allosteric stimulation by ...
A: The important regulatory enzyme of Glycolysis is PFK-1 and at this stage one ATP is generated. In ca...
Q: What biotechnology product that has improved life, and what would our life be without this product?
A: Biotechnology is a technology that uses living beings and biological processes to bring new solution...
Q: Deoxyribonucleotides a) Lack a hydroxyl group at position 2 of the sugar molecule b) Lack a hydrox...
A: Nucleotides are composed of nitrogenous base, a pentose sugar and a phosphate. DNA contains deoxyrib...
Q: Give examples of four different types of bond involving hydrogen atoms. (polar covalent, non-polar c...
A: Atoms in various molecules and compounds join together by forming a chemical bond. It is a very stro...
Q: which one is a part of protein coenzyme Select one: a. sodium b. sulphur c. copper d. calcium
A: Coenzyme: It is a small non-protein low-molecular-weight molecule that is bound tightly loosely to ...
Q: Which of the following is not a component of a nucleotide? a monosaccharide a phosphate a d...
A: A nucleotide must have a Phosphate group, Monosaccharide sugar and a Nitrogenous base.
Q: Protein folding
A: proteins are composed of some 20 types of amino acid residues, and they would be more or less alike ...
Q: 2. Consider the following pigments. OH HO NO G O,N NO (a) Provide the mechanism for the chemical rea...
A: The pigment in the question are Azo pigmnet/dye and is organic compound contains Azo group (-N=N-). ...
Q: Create 5 water molecules with appropriate bond formation. You must Include images of the water molec...
A: The main constituent of Earth's hydrosphere is water and all Known living organisms' fluid ...
Q: The fact the DNA bears the genetic information of an organism implies that A Base composition should...
A: All living organisms have cells, which are the most basic and essential unit of life. Cells are resp...
Q: B- what different between organic compound and inorganic compound ? with two example for each one. *
A: The level of organization of the human body from lowest to highest level includes atoms, molecules, ...
Q: Reset Double-helical DNA Sugar-phosphate backbone (RNA) Base uracil in Phosphate group Single-strand...
A: S.No. DNA RNA 1 Deoxyribonucleic Acid. Ribonucleic Acid. 2 Generally double stra...
Q: The following reaction coordinate diagram charts the energy of a substrate molecule (S) as it passes...
A: The catalysis of processes by enzymes is critical in living systems. Each organism goes through bill...
Q: Allosteric enzymes forms which shape of curve? O Linear O Hyperbolic O Bell-shaped Sigmoid shaped No...
A: Enzymes are biological catalysts that help organisms speed up chemical processes. Enzymes are protei...
1. Suppose you received two cultures from a reactor
biological: one containing a species of Gram negative bacteria and the other, a
species of Archaea. Discuss four different ways to distinguish these
cultures, without considering ribosomal RNA sequencing.
Step by step
Solved in 2 steps
- 100 3. A student performs an experiment similar to the one performed by Luria-Delbruck. However, the writing on the tubes became smudged. Which of these experiment supports the fact that mutations are spontaneous? Why? Growth in nonselective medium Growth in nonselective medium Isolation of micromycella and culture in selective medium Isolation of mkkromycelia and culture in selective mediam7a. A petri plate is given to you with 80 colonies on it. I tell you that I did five 10x dilutions and then plated 0.1 ml from my last tube to get 80 colonies on a plate. How many living bacteria per ml were in that original sample? Draw out (or describe) all of the dilutions I would have done to get this number. 7b. Again, I have a plate with 80 colonies on it, but I tell you that I did one 100x dilution and two 10x dilutions before I plated that 0.1ml from my last tube. How many living bacteria per ml were in that original sample? Draw out (or describe) all of the dilutions I would have done to get this number. please include drawings. thank you1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…
- 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.How does the streak plate technique help in isolating individual colonies of bacteria? Why streak multiple times on one plate? 3. While observing a plate inoculated with bacteria you observe a white-tan fuzzy growth. What can you interpret from this observation? Explain how this outcome could’ve occurred. 4. What are 3 possible errors that could take place as you culture your samples which could lead to contamination? State 3 and explain why. Microbiology class4. You have been given 6 new strains of Bacillus species recently isolated from a mining site. In order to compare the growth patterns of these strains, you set up a 24 hour growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and samples were taken after 24 hours. Each sample was then serially diluted from 10-¹ to 104, and 100 µl aliquots from each plated onto nutrient agar plates for enumeration (using the spread plate method). The plates were incubated overnight and the colony counts recorded in the table below. Strain number B1 B2 B3 B4 B5 B6 10-¹ TMTC 935 TMTC TMTC 156 570 B1 B2 B3 B4 B5 10-² B6 TMTC 99 391 TMTC 21 67 Dilution 10-3 786 25 48 670 4 12 10-4 220 Note: TMTC = Too Many To Count Complete the table below by calculating the number of CFU/ml for each of strains listed above Strain number CFU/ml 0 4 112 0 0
- 1. Table 1 lists a typical recipe for growing bacteria in the lab. A researcher discovered a potentially new species of bacterium from a soil sample and attempted to grow it in this media. Unfortunately, the bacterium did not grow. Identify at least three components that you suggest adding to the medium to enable growth. Provide a reason for adding each component. Table 1. per 1000 mL 1g Bacteria culture media NazHPO4•7H2O KH2PO4 3g 5 g 1 g 0.4% (wt/vol) 0.2% (wt/vol) NaCl NHẠC1 Glucose Casamino acidsWhich of the following is NOT correctly matched? A. Halophile – microbe that can grow in an environment with high osmotic pressure (e.g., salt) B. None of the other four answers (all are correctly matched) C. Agar – complex polysaccharide from seaweed used as a solidifying agent in culture media D. Colony – a population of microbial cells arising from a single cell or spore or from a group of attached cells E. Capnophile – microbe requiring an elevated molecular oxygen (O2) environment for optimal growth6a. A sample is given to you that contains 6 million cells per ml. You must make a series of serial dilutions in order to get a countable number on a plate to prove that there are indeed 6 million cells per ml. The plate that you eventually put a sample of bacteria on must have between 10 and 100 colonies for ease of counting. What dilutions do you make to get this many colonies on a plate? How many colonies do you have on that plate? Show what dilutions you make (Write exactly how much liquid you move into each test tube and how much liquid was in the test tube, ex. Move 1 ml of culture into 9 ml of water – 10x dilution). Don’t forget to tell me how much liquid you eventually put on your plate. (If you want to draw something out, you could draw then take a picture of it with your phone and submit the image along with the worksheet). 6b. Do the same problem as in 6a, but now you have to do it in 3 test tubes or less. What dilutions do you do now? please use drawings to help…
- 2. The following is an incomplete schematic diagram for the isolation procedure used. Label each step with the reagents used and products obtained. Yeast (a). heat, centrifuge residue 1 (discard) supernate 1 A) (b) pH, centrifuge residue 2 (discard) supernate 2 B) evaporate, filter residue 3 (discard) supernate 3 Cool to 40°C + c) + conc. HCI residue 4 supernate 4 alcohol + d) residue 5 supernate 5 C) D) Legend: a,b,c,d : reagents added A,B,C,D : products obtained. Assume you are given two cultures, one of a species of gramnegative Bacteria and one of a species of Archaea. Discuss at leastfour different ways you could tell which culture was which.8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant. Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. 200%- 927 PM 0 日 2/7/2021 21 DELL Insert Delete F11 F12 PrtScr F9 F10 F7 F8 F6 F3 F4 F5 Esc F1 F2 Backspace & # 2$ 8 4 1 2 P Home R T E Enter K Shift V B Page Down Page Up Alt Ctrl Alt THome ノ/ *00 Σ