+ DNA Fragment with Human Gene Recombinant Plasmid Plasmid Vector Bacterial DNA Recombinant Plasmid Transformed Bacterial Cell Bacterial Cell Produces Human Protein Genes for medically important proteins can be cloned and inserted into bacteria resulting in recombinant bacterial DNA, as shown in the diagram below Why can bacteria recognize a human gene and then produce a human protein? O Bacterial cells and human cells contain the same kind of chromosomes Bacterial cells contain the same organelles as human cells O DNA replication in bacteria and humans is the same The basic components of DNA are the same in humans and bacteria 이

Q: Which mechanism is utilized to replicate plasmids undergoing conjugal transfer? Rolling-circle…

A: Replication  Replication is the process where DNA materials gets doubled in a cell.

Q: dye-linked substrate called X-gal (5-bromo-4-chloro-3-indolyl-ß-D-galacto-pyranoside) into galactose…

A: The lac operon contains a group of related genes (promoter, operator, Lac Z, Lac A, and lac Y) that…

Q: All of the following lead to horizontal gene transfer EXCEPT plasmids transfer of genetic material…

A: Gene transfer refers to the movement of genetic information between organisms. Gene transfer can be…

Q: Colonies/ug of plasmid) based on these results? O about 140O O about 800 O about 4000 O about 400…

A: INTRODUCTION Transformation efficiency is that the efficiency by which cells can take up…

Q: Which statement is NOT the function of restriction enzyme?

A: Answer: It ligates the desired to gene to vector’s plasmid.

Q: Discuss three stages of TRANSFORMATION and RESTRICTION ANALYSIS OF PLASMID DNA experiment. What…

A: For transformation and restriction digestion analysis follow the given steps:  1. Cut the plasmid…

Q: Please help Why did we use biodegradable nanoparticles? Please use The worksheet below and don’t…

A: Biodegradable Nanoparticles (BNPs) are basically particles with matter of interest (such as gene…

Q: DNA polymerase I DNA polymerase II DNA ligase Primase RNA primer 5' Lagging strand 3' 3' Okazaki…

A: The process of copying of double stranded DNA by the application of several Enzymes and proteins…

Q: Look at the double-stranded segment of DNA shown below. Imagine that the two strands have already…

A: DNA is the nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid in which…

Q: Using the picture below, match each letter (A-E) to 5' or 3' DNA polymerase molecule Parental DNA…

A: As the options are not visible, we are answering the question based on the general principle of DNA…

Q: In order to construct a genome library, which statement most accurately describes the correct order…

A: Genomic DNA is an entity's chromosomal DNA that serves as a representation of the entirety of its…

Q: Although many cloning applications involve introducing recombinant DNA into bacterial host cells,…

A: Cloning can be defined as the process by which identical copies of the organisms are created. More…

Q: In the gel electrophoresis: the mutant would just run the same as the open circle DNA if it simply…

A: In gel electrophoresis, the behavior of mutant DNA in comparison to open circle DNA and the presence…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Plasmid DNA In contrast to a cell's chromosomal DNA, plasmidsare tiny, circular, double-stranded DNA…

Q: Which of the following is/are true of DNA general recombination? The process involves invasion of a…

A: DNA recombination in general is a natural process that occurs in a wide range of organisms,…

Q: Im designing a restriction cloning experiment. My professor said that plasmid and insert DNA work…

A: It would have been simpler if the digested product had been visible at a lower point on the gel.…

Q: What is the following molecules not required for DNA synthesis during S- phase? * O RNA primer…

A: DNA replication is the process by which new DNA is synthesized from the old DNA. In case of…

Q: After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that…

A: Bacterial transformation is a process through which bacteria can take up foreign DNA. Transformation…

Q: What's the cloning process from the first step to the last? Put the steps in order based on the…

A: Cloning is a technique for creating a genetically identical copy of a cell or organism. It is a…

Q: Small extrachromosomal rings of DNA, often contain genes that give bacterial cells genetic…

A: Small extrachromosomal rings of DNA, often contain genes that give bacterial cells genetic advantage…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Restriction enzymes These are proteins that are isolated from the bacteria that cleave the sequences…

Q: What is the DNA template of the following DNA coding:  ATGGCTAACCTTGTA

A: The DNA template strand is a complementary strand to the DNA coding (sense) strand. It serves as a…

Q: Order the following steps according to the Sanger sequencing method The bands in the electrophoresis…

A: DNA sequencing is a technique used to evaluate the exact nucleotide bases , their position as well…

Q: С 17 Based on the knowledge you gained from the cloning module, which of the bands in the figure is…

A: Agarose gel electrophoresis is a method used in molecular biology to resolve DNA fragments on the…

Q: The mini-prep silica column will preferentially bind deoxyribonucleotides. How is it that at the enc…

A: An alkaline solution containing sodium dodecyl sulfate (SDS) is then added to facilitate cell lysis…

Q: 3000 bp 2000 bp 1000 bp 800 bp 700 bp 500 bp L Uncut EcoRI BamHI Ncol EcoR1/BamH1 BamHI/Nco1…

A: Plasmids are double-stranded circular DNA molecules. Due to their small size and stability, these…

Q: Match the following (most appropriate combinations): helicase Topoisomerase DNA Polymerase III DNA…

A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around…

Q: Give typed full explanation  you made a recombinant plasmid (it is circular) that contains the…

A: Here, the correct answer is option 1) The plasmid will be cut once, making a linear DNA fragment.

Q: Which of the following best explains the natural function to the tumor-inducing (Ti) plasmid? It…

A: The deoxyribonucleic acid (DNA) is the double-stranded molecule that is the genetic material in most…

Q: How many DNA fragments will result when the double-stranded DNA molecule below is digested with…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Plasmids are circular double-stranded DNA elements. These are found in prokaryotes. The plasmids…

Q: Below is a picture that depicts transcription. Shown is the DNA double helix, the replication…

A: Transcription is the process where the mRNA sequence complementary to the template strand is…

Q: You transform tetracycline sensitive competent cells with a plasmid solution (the plasmid encodes…

A: Tetracyclines were discovered in the 1940s and exhibited activity against a wide range of…

Q: Transcription V [Choose ] Coagulase RNA polymerase Translation DNA polymerse Ribosome Reverse…

A: The central dogma of molecular biology suggests the flow of genetic information from DNA to rna to…

Q: 1) Follow the steps involved in DNA or gene cloning

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: During which step in the transformation protocol does the plasmid DNA move into the cell? O Heat…

A: During a procedure termed transformation, bacteria may pick up foreign DNA. In DNA cloning,…

Q: Choose the false statement. O Genetic engineering includes recombinant DNA techniques whereby DNA is…

A: Genetic engineering is also known as the recombinant DNA technology.It involves the use of set of…

Q: Bacteriophage T4 has a linear double-stranded DNA genome, yet mapping many mutations, as shown in…

A: T4 bacteriophage infects E.coli. It has a double stranded linear DNA genome. It undergoes a lytic…

Q: r it 6- Complete the following: a) Two fragments of DNA can be joined by a b) Restriction enzymes…

A: Recombinant DNA technology is the process by which we can isolate our gene of interest and then…

Q: Using the figure below, what is molecule "A" (type a 1, 2 or 3 in the blank)

A: This question is based on the Dna replication.

Q: Which of the following things must happen first if DNA is going to be replicated * O DNA gets broken…

A: DNA replication is the process which is responsible for producing the daughter ds DNA molecule from…

Q: 3 in 3' 5' 5' 3'

A: DNA replication is the process of duplicating DNA to form two identical copies. It takes place in…

Q: 3' Shown is a segment of DNA about to be replicated. a) What is the sequence of the complementary…

A: DNA replication is a biological process that occurs in all living organisms and is the basis for…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.