+ DNA Fragment with Human Gene Recombinant Plasmid Plasmid Vector Bacterial DNA Recombinant Plasmid Transformed Bacterial Cell Bacterial Cell Produces Human Protein Genes for medically important proteins can be cloned and inserted into bacteria resulting in recombinant bacterial DNA, as shown in the diagram below Why can bacteria recognize a human gene and then produce a human protein? O Bacterial cells and human cells contain the same kind of chromosomes Bacterial cells contain the same organelles as human cells O DNA replication in bacteria and humans is the same The basic components of DNA are the same in humans and bacteria 이

Match the enzyme on the left with its role in DNA replication DNA polymerase I helicase DNA ligase DNA polymerase III topoisomerase primase 72 W w# 3 E $ 4 R % 5 T A 6 MacBook Pro Y & 7 U * 8 replaces primers with DNA connects Okazaki fragments to form a continuous strand of DNA synthesizes short RNA fragments used to initiate DNA synthesis Uses the 3'OH of an RNA primer to synthesize the leading strand and Okazaki fragments keeps DNA from getting tangled up ahead of the replication fork "unwinds" the DNA double helix at the origin and replication forks 1 ( 9 X 0 0 P + 11 Next

The image below shows the replication bubble of a piece of DNA in the process of replication. However, the image only shows the DNA strands being replicated. Fill in the rest of the elements of the figure, specifically: primers, Okazaki fragments, newly replicated leading strand DNA, as well as the enzymes helicase, primase, DNA polymerase III, DNA polymerase I and ligase. Also be sure to indicate the 5’ and 3’ ends of all nucleic acid polymers.

draw structure of plasmid

Clone number in this case is number 196 as shown in the images. What is the exact length of the segment of human DNA that has been inserted into the plasmid? *report the entire length of the insert, not just the sequences matching the ends and labels of wells isn't needed for answer*

You first need to create a plasmid. What are the minimal components necessary to develop this plasmid? In addition to these components, please draw the plasmid. In this illustration, I am looking for the components, the direction of transcription (i.e., the direction the genes will be transcribed), and what should be transcribed last? Also, where would you specifically insert the P. falciparum gene in this plasmid, and why are you checking reading frames in your gene? Finally, please name your plasmid using the correct nomenclature too.  You are excited you have this new plasmid; you want to transfect it into P. marinus and provide it as an oral vaccine to laboratory mice. However, even though your supervisor enjoys your enthusiastic attitude, they do not allow you to do this quite yet because all you have is this plasmid. Why doesn’t your supervisor allow you to use the laboratory mouse for this research regarding animal welfare guidelines? Your answer should include the 3 R’s of…

Part A How is a gene for a particular protein inserted into a plasmid? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. phosphate groups sticky ends an apurinic enzyme nitrogen-containing bases a recombinant DNA joined Submit cut ovide Feedback a donor DNA a restriction enzyme Request Answer When a gene has been obtained using Reset Help it is mixed with the plasmids that have been by the same enzyme. When mixed together in a fresh E. coli culture, the of the DNA fragments bond with the of the plasmid DNA to form

Consider the ends of the DNA fragments shown below. They have been produced by digestion of a single sequence of DNA using a number of restriction endonucleases. 1. 5'A         3' 3'TTCGA5' 2. 5'G          3' 3'CAGCT5'     3. 5'AATTC3' 3'         G5 4. 5'TCGAC3' 3'         G5'     5. 5'GGG 3' 3'CCC 5'   Which of these ends are capable of annealing and being joined by DNA ligase?