Devise a scheme to prepare a 10-9 dilution spread plate, using the least number of dilution tubes. Note: It is not practical to pipette any volume less than 0.1 ml and your equipment is similar to that in the Micro-lab, you only have 10 ml dilution tubes and a supply of sterile water

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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  1. Devise a scheme to prepare a 10-9 dilution spread plate, using the least number of dilution tubes. Note: It is not practical to pipette any volume less than 0.1 ml and your equipment is similar to that in the Micro-lab, you only have 10 ml dilution tubes and a supply of sterile water
  2. Describe the growth patterns of each bacterial isolate that you observed in the nutrient agar deeps (shake cultures)  and state what this says about their metabolism. Give definitions of these bacterial growth in relation to O2
  3. E. coli:  turbid growth throughout 

     

     

     

    Micrococcus luteus:Growth on the top

     

     

     


    Clostridium sporogenes: growth on the bottom

     

 

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