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Depending upon the structure of cell wall, bacteria are divided into two major classes. The basis of this division is Gram staining. Gram staining is a differential staining method used to classify bacteria as either Gram positive or Gram negative. The main differentiating characteristic feature is that gram positive bacteria have thicker peptidoglycan layer in comparison to gram negative bacteria.
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- Question 3 If the stain is too red to the naked eye, what can be done to correct this? O that is normal O check pH and decrease it if it is required O decrease staining time O increase staining time Question 4QUESTION 5 Regarding the Gram stain procedure: whether Gram-positive or Gram-negative, cells may appear colorless... O A. prior to application of the primary stain OB. prior to application of the counterstain O C. following application of the mordant O D. A. B, and C are all true O E. Only A and B are true O F. Only B and C are trueThe significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.
- Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible66671/take/questions/800382 Regarding Gram staining, what will be the appearance of the Gram-negative bacteria if... All steps are done correctly? [Choose ] [Choose ] Pink The slide is not heat-fixed prior to Purple staining? Clear Orange Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? Not enough acetone:alcohol was used? Carbol fuschin was omitted? Carbol fuschin or safranin was omitted? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]Question 2. After sterility test of an injectable it was observed that an injection is failed the test what will you do then depending on the product nature (heat sensitive/ heat resistant) (Not more than one sentence)? Mention the purpose of product inhibition test and how it is performed (Not more than one sentence) ? How will you check the validity of a medium (Not more than one sentence) ?
- Question 14 Which of the following tests requires a dilution of the culture before inoculation? O Bacitracin Bile esculin agar O DNase O Coagulase1:07 < Вack Untitled 6 Brownian movement of bacteria: O a. Can be observed by the of soft agar technique O b. Can be observed by staining of Staphylococcus aureus Oc. Is common in Escherichia coli or Proteus vulgaris O d. Is common in Staphylococcus aureus O e. Can be observed at the edge of a microscope CLEAR MY CHOICEQuestion:- Your medical care team determines that a third patient's severe illness is caused bya helminth. Which technique or techniques described above would you use to identifythe helminth? Justify your answer.
- Answer the following questions: 1. Why do we pass the smear in the open flame of the bunsen burner 3 times? What do we want to achieve by doing this? 2. Is the size of the bacterial smear on the slide very critical to the analysis? Why or why not? 3. Is the thickness of the bacterial smear on the slide very critical to the analysis? Why or why not? 4. Why do we pass the smear in a gentle stream of water after flooding it with a stain? What do we want to prevent by doing this? 5. Is the duration or time of stain application very critical in simple staining? Why or why not? NOTE: Please try to answer all of the questions asked, i promise to give you a good ratingsD Question 15 Not yet answered Marked out of 1 Flag question Previous page 15°C Sunny 1080 Which of the following is a technique used to dissolve sterile solid dosage forms? Select one: O a. O b. Reconstitution O c. Levigation O d. Dissolution Trituration * Q Search LA D --- acerizzes/00071/take/questions/600381 Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? The slide is not heat-fixed prior to staining? Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? I Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? W [Choose ] [Choose ] Purple Clear Pink Orange [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 88 22°C