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Question:-
Starting with data collection, describe the steps necessary to determine a 3-Dimensional structure using electron microscopy methods.
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- Question:- What is the best superresolution microscopy method to resolve in z direction.Question:- calculate the distance between two ocular micrometer division markers in micrometers (μm) when viewed at 100x total magnification, then at 400x.PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…
- Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…answer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:Previous question: The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. The image shows the 1mm is for this question. - Answer for this question was 3mm.
- SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…Direction: Read and analyze the following laboratory experiment and answer the following question. PART 3: PLASMOLYSIS Materials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps, compound microscope, iodine, small knife, water, salt (5% and 10% solution) Methodology: 1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife. 2. Use an eye dropper to place a drop of water in the center of a microscope slide. Use the tweezers to peel a thin layer of skin tissue from the thick part of the onion wedge and place it in the center of the microscope slide. 3. 4. 5. Add a drop of water and a drop of iodine over the onion tissue on the slide. Carefully lower a cover glass slip at an angle on the stained tissue to allow air bubbles to escape. 6. Examine the prepared slide under the compound microscope at 100X magnification. 7. Record what the cells look like. 8. Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per 100…Analysis: Write your answers on the space provided. 1. How does the letter “e” as seen through the microscope differ from the way an “e” normally appears? 2. When you move the slide to the left, in what direction does the letter “e” appear to move? When you move it to the right? Up? Down? 3. How does the ink appear under the microscope compared to normal view? 4. Why does a specimen placed under the microscope have to be thin?
- Differentiate between bright-phase and dark-phase microscopy in terms of phase shift.Subject : Environmental Microbiology Can u use the information given below to answer these 2 question 1.Provide an aim for this lab 2. Provide objectives DISCUSSION QUESTIONS What is the relationship between the resolution power and the useful magnification that may be obtained with the light microscope? What determines the resolving power of the lens system? What is the limit of resolution obtainable with the light microscope? How you will distinguish between bright field and dark-field microscopy and provide a specific example where each would be method of choice for observing a culture of bacteria? What advantages does electron microscopy have over light microscopy? What are disadvantages of electron microscopy over light microscopy? #Compare the use and the methodology of TEM with SEM? Provide at least one example where each would be the method of choicES Practice, Question 07 Select ALL the correctly described types of microscopy: Bright field commonest microscopy using visible light O Dark field/ uses UV light and spotlights specimens O Nomarski/ uses dual beam visible light with high resolution but shallow depth of field O Phase contrast/ dual beam light, contrast comes from phase changes within the specimen 1 2 13 14 15 O Fluorescence/ uses gamma rays to resolve specimen detail 16 17 18 19 20 Study MAY 24 MacBook Pro 80 000 O O O O O