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Describe several ways in which coliform bacteria are known to get into well water. (Do
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- Describe the steps you would take to isolate, cultivate, and identify a microbial pathogen from a urine sample. (answer constructively)Write an abstract of the article below in Methods (around 3 sentences): METHODSTo accomplish this, three kinds of inocula - Pond bottom soil 1, Pond bottom soil 2 and Horse dung - wereobtained from two different ponds and outdoor loafing sheds near Keller, Texas. Waste samples, including food,paper and yard waste, were collected from a house. Three laboratory scale reactors were prepared with selectedhousehold waste and inoculum with the required amount of water and operated at room temperature. Duplicateruns were conducted for the first set of experiments. The pH level of reactors was controlled by adding sodiumhydroxide.Briefly explain how you can determine the presence of coliform bacteria in a water sample.
- Suppose you observed a bacterium growing on Mannitol Salt agar (MSA) with a yellow colony appearance in the clinical microbiology lab. Name the genus and species you are most likely observing; state its most likely oxygen needs and salt requirements; and predict if this organism would grow on MacConkey agar or Colistin Nalidixic Acid agar. If you want to control the growth of this organism in a healthcare setting, predict what disinfectants would be best to use and their modes of action. Explain your reasoningAnother method of microbial control is filtration. List down at least 5 kinds of filters which could be used for this method?Give the standard scientific definition of “coliform”. Are coliforms indicator organisms (YES OR NO)? Explain why the total bacterial acceptable levels are higher than the coliform acceptable levels.
- As a microbiology student, how can you prevent or control coliform contamination in drinking water of your respective communityA chemostat creates a constant flow of fresh nutrients and waste removal for industrial use of bacteria. True FalseDescribe the purpose of identifying unknown bacterial cultures in medical field. Name the step by step experiments/procedures that microbiologists follow in order to identify microorganisms.
- Answer the question briefly and concisely. The Mannitol salt agar (MSA) only allows the growth of halophiles (salt-loving microbes);nonhalophiles will not grow. Among the halophiles, mannitol fermenters will produce acid that turnsthe pH indicator yellow; mannitol nonfermenters leave the medium red. Onto MSA you inoculate ahalophilic mannitol fermenter and a halophilic mannitol nonfermenter. In this case, the medium isacting as (a) __________ medium(s).A. SelectiveB. DifferentialC. Selective and differentialD. EnrichedA patient is diagnosed with a urinary tract infection and a urine sample is obtained and sent to the microbiology lab. The urine sample is inoculated to agar plates and after 24 hours of incubation bacterial colonies are present on the plates. Draw what the bacterial growth on the agar plate looks like.Watch this video and answer the following questions: https://www.youtube.com/watch?v=PbgB2TaYhio What was missing in Dr. Llyod's Petri dish experiment for the microbes to grow? How much time is it estimated that these microbes would need to grow? What would be one biotechnological application afforded by learning more about deep subsurface microbes?