PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_The field of view (FOV) is the circular area you can see when you look through the microscope. Thediameter of the field of view is different depending on which objective lens you are using. For example,you are using the medium-power objective lens, then the area you can see is actually smaller than if youwere using the low-power objective lens. Knowing The diameter of the field of view can help you estimateactual size of objects / cells seen through the microscope.When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1).Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the leftedge of the field of view (see figure 2).NOTE:Slage cipI-1000Objects in the FOV are usuallymeasured in micrometers (um).To convert, a FOV in mm, timesit by 1000 to get um.(Eg. 0.8mm x 1000 = 800 um).1400 mRulerA mm-Raler EdeeEigureStage Sagu oponingEigure 2Refer to Figure 2 above and measure the diameter of the LOW POWER.field.of.view.using the transparant.ruler. Each space between the darklines represents1 mm. Determine the amount of spaces you see and write this in the chart below. Now measure the diameter of the MEDIUM.andHIGH POWER feld of views using the formulas indicated.".FOV DiameterMeasurementMeasurement(mm)FORMULANOTE:LOW POWER FOvOnly the lowpower FOV can beMeasured using a rulermeasuredaccurately using aruler. You will needto use a formula tocalculate thediameter of the FOVunder medium andhigh power.low power FOV.imm total low power MAGtotal medium power MAGMEDIUM POWER FOVHIGH POWER FOVlow power FOV immix total low power MAGtotal high power MAGCalculate the diameter of the Field of View (FOV) on low power for each diagram which shows the lines of a rulerA.B.C.

The field of view (fov) of a low power objective can be find by calculating the the diameter of…

Refer to Figure 2 above and measure the diameter of the LOW. POWER feld.afvieusing the trannparant.nder. Each space between the dark Ines represents 1 mm. Determine the amount of spaces you see and write this in the chart below. Now measure the diameter of the MEDIUM.and HIGH POWER feld at ienauning hafarmdan indicated." FOV Diameter Measurement FORMULA Measurement (mm) NOTE: Only the low power FOV can be measured accurately using a ruler. You will need to use a fommula to calculate the diameter of the FOV under medium and high power. LOW POWER FOV Measured using a ruler MEDIUM POWER FOV low power FOV.mm a total low power MAG total medium power MAG lew.powar FOV im letal low power MAG total high power MAG HIGH POWER FOv

Refer to Figure 2 above and measure the diameter of the LOW. POWER feld.afvieusing the trannparant.nder. Each space between the dark Ines represents 1 mm. Determine the amount of spaces you see and write this in the chart below. Now measure the diameter of the MEDIUM.and HIGH POWER feld at ienauning hafarmdan indicated." FOV Diameter Measurement FORMULA Measurement (mm) NOTE: Only the low power FOV can be measured accurately using a ruler. You will need to use a fommula to calculate the diameter of the FOV under medium and high power. LOW POWER FOV Measured using a ruler MEDIUM POWER FOV low power FOV.mm a total low power MAG total medium power MAG lew.powar FOV im letal low power MAG total high power MAG HIGH POWER FOv

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter15: Instrumentation In Medical Imaging

Section: Chapter Questions

Problem 9P

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Previous question: The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. The image shows the 1mm is for this question. - Answer for this question was 3mm.
3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?
These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm
Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…
Previous question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.
Viewing Object Through the Microscope: If the diameter of the low-power field is 1.5mm, an object that occupies approximately a third of that field has an estimated diameter of _______mm.
Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4,500 um Scanning (40X): 1.8 mm x 100X/40X = 5 mm = %3D Low power (100X): FOV diameter = 1.8 mm = 1,800 um 1.8 mm x 100X/400X = 0.45 mm =450 um 180 um High power (400X): 1.8 mm x 100X/1000x = 28 mm = Oil immersion (1000X): %3D Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification FOV diam. um FOV diam. um length um length um
The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?
Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
The objective lenses of the microscopes used in this course are all parfocal. What does having parfocal objective lenses mean? Question options: The slide has to be moved away from the objective lens (i.e., lowered) when switching between objective lenses. None of these choices The objective lenses have optimized light gathering capability. Once in focus, you may switch between objective lenses with only fine focus adjustment required.
PRELAB: Calculating Magnification Each lens makes the object look larger by a specified amount. To determine the total magnification, multiply the magnification of the eyepiece and the objective being used. Table 1: Microscope Magnification Magnifying power Objective Objective Eyepiece Total name Scanner 4 X Low power 100 X High power 40 χ PRELAB: Estimating the Size of an Object using a Microscope You will not be able to use a ruler to directly measure the size of a cell. Instead, you will use an indirect method by comparing the cell to the size of something you already know. The diameter of the microscope's field of view as seen through the eyepiece is a convenient length to use. A very small unit of measurement, the micron (µm) is used for measuring microscopic objects. The conversions are: 1 um = 0.001 mm 1 mm = 1000 µm Table 2: Field of View Diameters Objective Diameter name millimeter (mm) micron (um) Scanner 4 Low power High power 0.5 To estimate the size of an object, divide…
when preparing the slide. The stage micrometer measures in millimetres. Figure 1 shows the student's eyepiece graticule and stage micrometer. The student used an eyepiece graticule to calculate the size of some of the onion cells. Figure 1 10 20 30 40 Eyepiece graticule 0 mm Stage micrometer Use Figure 1 to calculate the size of one division on the student's eyepiece graticule, in micrometres. Show your working.