Describe in discrete steps how a polyadenylation signal contributes to the formation of a mature mRNA with a poly A tail of more than 300 nucleotides long
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Q: Describe the significance of a 5 cap poly A tail on the mature mRNA
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Describe in discrete steps how a polyadenylation signal contributes to the formation of a mature mRNA with a poly A tail of more than 300
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- What is the total size of the mature i.e. fully processed mRNA in nucleotides? How many amino acids would the encoded protein be? Assume that the N- terminal Met encoded by the AUG start codon, is NOT cleaved from the protein?Given the following mRNA transcript: 5’-UUUGGCAUGGGUAUCGUAGAGAUGGAAUUCAUAGUGGAGUAA-3’ What is the one-letter abbreviation of the protein product of the mRNA transcript?Which of the followings indicate the order of procaryotic mRNA degreadation? cleavage of the triphosphate 5′ terminus to yield a monophosphate- 3′ to 5′exonuclease digestion- The endonucleolytic cleavages occur in a 5′ to 3′ direction on the mRNA following the passage of the last ribosme cleavage of the triphosphate 5′ terminus to yield a monophosphate- The endonucleolytic cleavages occur in a 5′ to 3′ direction on the mRNA following the passage of the last ribosme- 3′ to 5′exonuclease digestion The endonucleolytic cleavages occur in a 5′ to 3′ direction on the mRNA following the passage of the last ribosme- cleavage of the triphosphate 5′ terminus to yield a monophosphate- 3′ to 5′exonuclease digestion
- Explain why RISC binds to a specific mRNA. What type of bonding occurs?What is the mechanism for addition of a guanosine to create the 5' methyl cap of a mRNA? O The 5'-OH end of the nascent mRNA performs a nucleophilic attack on the gamma phosphate of GTP O The gamma phosphate is removed from the 3' end of the nascent MRNA and then the beta-phosphate attacks the phosphorus atom of the alpha- phosphate of GTP O The gamma phosphate is removed from the 5' end of the nascent mRNA and then the -OH group from the beta-phosphate attacks the phosphorus atom of the alpha- phosphate of GTP O The 5'-OH end of the beta phosphate present on nascent mRNA performs a nucleophilic attack on the alpha-phosphate of GTPDescribe some of the ways in which mRNAs might regulate gene expression. What is the role of poly(A) in mRNA stability? How might the cell regulate the stability Different mRNAs?
- For the following mRNA sequences, what is the amino acid sequence formed? Consult genetic code table for reference. 5' AGUCCGUAC 3' 5' AAUUGCUUC 3'Describe the significance of a 5 cap poly A tail on the mature mRNADoes RNA Polymerase II terminate transcription at the polyadenylation signal? Describe in discrete steps how a polyadenylation signal contributes to the formation of a mature mRNA with a poly A tail of more than 300 nucleotides long How does this poly A tail affect the mature mRNA?
- Provide the abbreviation for the amino acid sequence expected from the following mRNA segment using the three-letter amino acid codes: 5' UUUICCCIAAUIAUUIACG 3'In eukaryotes there is not a consistent relationship between the length of the coding sequence of a gene and the length of the mature mRNA it encodes, even though one nucleotide in DNA = one nucleotide in pre-mRNA or primary transcript. Explain why this is so.The base sequence of the gene coding for a short polypeptide is 5’CTACGCTAGGCGATTGATCATC’3. What would be the base sequence of the mRNA transcribed from this gene? Highlight the start codon sequence State the amino acid sequence of the polypeptide translated from this mRNA Based on the information from part (a) and (b), describe the process used by eukaryotes to produce protein.