David Pribnow studied the base sequences of promoters in bacteria and bacterial viruses. He found two conserved regions in these promoters (the -10 box and the -35 box). These two regions of the promoter Select one: O a. signal the initiation site O b. bind the sigma subunit that is associated with RNA polymerase O c. attach the correct nucleotide triphosphate to the template DNA strand O d. separate the two DNA strands

(11) If you design a gRNA with the sequence, GGU AUC AUU GCA CUG ACC AG, in theory which position of the nucleotide in the genomic DNA sequence below will be cut by Cas9 protein, which may lead to a knockout due to an error in DNA repair? The starting and the end coordinate positions of the DNA fragment on the chromosome are indicated at the beginning and end of the sequence, respectively. 110,001 ATC GGT ATC ATT GCA CTG ACC AGA GGC TAC 110,030 A) Between 110,023 (G) and 110,024 (A) B) Between 110,021 (C) and 110,022 (A) C) Between 110,020 (C) and 110,021 (C) D) Between 110,009 (C) and 110,010 (A)

Considering only the listed genetic elements in the figure below, how many different mRNAs and polypeptides can be made from this DNA fragment? Promoter start Codon Transcription terminator STOP Start STOP codon Lodon codon O A. 1 mRNA and 3 polypeptides B. 3 mRNA and 3 polypeptides C. 1 mRNA and 2 polypeptides D. 2 mRNA and 3 polypeptides Promoter Start codon Transcription terminator STOP codon

Which DNA strand will serve as the template strand during the transcription of the RNA-coding sequence?