Culture

Q: Experiment of batch fermentation of E. coli in a stirred tank bioreactor was carried out.  0.5g / L,…

A: Scale  On X axis, 2cm= 0.5 hours On Y axis, 1cm= 0.02 OD

Q: What terms are used to describe organisms whose growthpH optimum is very high? Very low?

A: In the living world, we see a large number of microorganisms, animals and plants. These organisms…

Q: Explain the imporance of using a control tube with only broth when measuring turbidity in a…

A: Introduction :- There are two types of growth medium or media which are used to grow microorganisms…

Q: distinguish between selective, differential, enriched, and minimal media. Give examples of…

A: Introduction  Microbiological media, often known as bacterial culture media, is a bacterial growth…

Q: a) Draw the diagram and describe motility required for the bacteria that have flagella to an…

A: Bacteria are a diverse group of microscopic, unicellular organisms that are categorized as…

Q: Calculate mean growth rate and generation time of a culture that increase in exponential phase from…

A: Generation time is time taken for culture organisms to double in size. Generation time = t/n  where…

Q: Calculate doubling time (td) and specific growth rate (μ) by using logarithmic growth stage.

A: In unicellular organisms, growth is measured in terms of the increase in the number of cells by cell…

Q: esence or absence of oxygen. After 12 hours, all of the glucose in each culture has been ned. You…

A: 1) The cell culture grown aerobically with the presence of oxygen will have more cell density than…

Q: Pseudomonas aeruginosa is capable of aerobic respiration and anaerobic respiration but not…

A: SIM test stands for Sulfur, Indole and Motility test. Hence, this test allows the detection of…

Q: Was there any difference in the amount of gas produced by yeast in room temperature tapwater…

A: Saccharomyces cerevisiae, more commonly known as Baker’s Yeast, is a single-celled microorganism,…

Q: Photomicrograph (in HPO) of Hydrilla. Give an objective description of the plant cell.

A: * Hydrilla is genus of aquatic plant contain only one species called Hydrilla verticillata. * But…

Q: Is generation time a useful parameter to indicate the types of mediabest suited to support the…

A: The advancement in science led to the formulation of several growth mediums that could support the…

Q: Calculate the growth rate and generation time of a culture that increases in the exponential phase…

A: The growth of bacteria over a period of time is represented by a growth curve. This curve tells the…

Q: Explain the changes in the amount of nitrate and carbon dioxide in the graph below. Guide questions:…

A: Nitrogen cycle depicts the biochemical changes that occur where nitrogen is converted to various…

Q: The term psychrophile? a. refers to an organism that has an optimum growth within the range of -5…

A: Bacteria  A unicellular prokaryotic microorganisms not visible to the naked eyes.

Q: Explain why you can use OD675 (optical density at a wavelength of 675nm) as a proxy for cell density…

A: Algae are group of predominately aquatic, photosynthetic, and nucleus bearing organism lacking true…

Q: Enter points on the axes and graph the probable results for anacidophile, a neutrophile, and an…

A: Microorganisms growing optimally in the ph of less than 5.5, are known as Acidophiles. For example…

Q: After performing surface sterilisation on leaves, the leaf explants were cultured on a medium…

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Q: growth curve

A: Note: As per the guidelines of bartleby we have to answer the first 3 questions as they are…

Q: What kind of ion exchange resin would be most effective for purifying RNA molecules, an anion…

A: The purification of RNA molecules is a critical step in many molecular biology and biotechnology…

Q: 10. What is Relative Centrifugal Field (RCF, in g's) in a centrifuge with a rotor radius of 10 cm…

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Q: What is the equation that represents the mass balance of the exponential phase of bacterial growth?…

A: In exponential growth, a population's per capita (per individual) growth rate stays the same…

Q: Make a hypothetical graph showing the growth progression of lactic acid bacteria and coliforms in a…

A: Burong mustasa is a traditional Filipino dish produced by fermenting mustard leaves and stems…

Q: Place appropriate points on the axes and draw and label separategraphs that fit a psychrotroph and…

A: When microorganisms supplied with an adequate amount of nutrients and other environmental factors…

Q: Describe the similarities and the differences in the growth patterns in the two plates.

A: For optimal microbial growth, various growth conditions are required. Oxygen requirement is one of…

Q: Give only typing answer with explanation and conclusion What percentage of Vmax is achieved when the…

A: In research on enzyme kinetics, the connection between substrate concentration and reaction rate is…

Q: 3) If Hybridoma cells are cultured supported in an agarose matrix in which nutrients and oxygen are…

A: A hybrid cell utilized as the purpose for the generation of antibodies in huge quantities for…

Q: The death of Geobacillus stearothermophilus spores is routinely used to assess the effectiveness of…

A: Sterilization procedures are often assessed by looking its ability to kill Bacillus endospores.…

Q: Using your growth curve determine approximately how long is lag phase? The log phase? The stationary…

A: A Bacterial Growth is an increase in cell number by asexual processes of cell division in bacteria…

Q: Give typed explanation what is the best suspending agents for cyclosporin (suspension)? explain why…

A: Cyclosporin: Cyclosporin (also known as cyclosporine) is a medication used to prevent organ…

Q: Microbial Cell Density, Growth Curves Time (min) 0 20 40 60 80 100 Gp1 Abs 0.054 0.053 0.10 0.21…

A: The time taken by a population to double it's size is known as the doubling time. A semilog graph…

Q: What is the reason why using a spectrophotometer is considered an indirect measurement of cell…

A: A spectrophotometer is an instrument which is used to measure the amount of photons absorbed after…

Q: Calculate the mean generation time of a bacterium that increased its number from 1 x10° cells/ml to…

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Q: Name two biological processes that operate in a fixed film-growth mode?

A: This model considers secondary clarifier at a rural sewage treatment plant which is a typical phase…

Q: 6. Examine the following graph. Note that the growth rate increases slowly until the optimum is…

A: Every organism lives in a particular habitat. The characteristic conditions that determine the type…

Q: Describe methods that a cell may use to adapt to heavy metal contaminated sites. Define halophile.…

A: Introduction :- Stress proteins, also known as heat shock proteins or chaperones, are a group of…

Q: Calculate the amount of oxygen provided to an aerobic culture, expressed as mg O2/L of culture-hour,…

A: An aerobic culture is a kind of bacterial culture in which growth is allowed to occur in a state of…

Q: Answer the question briefly and concisely. The Mannitol salt agar (MSA) only allows the growth of…

A: Nutrient growth media are laboratory media that are used to support the growth of microorganisms,…

Q: After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to…

A: Pathogenic microorganisms exist as biofilms and form an extracellular matrix that protects from the…

Q: Description of how you have modified pour plate and streak culture method to see the effect of…

A: In microbiology lab, for the preparation of pure culture of a particular microorganism different…

Q: a. What biochemical events in quorum sensing ensure that a biofilmcan function as a unit?b. Explain…

A: For several decades microbiologists thought that bacterial populations are collections of…

Q: From the results shown in figure , determine which drugs couldbe used to treat the yeast infection.…

A: Fungi are the multicellular eukaryotic organisms. They are heterotrophs and contain chitin in their…

Q: All of the following are valid measurements of growth except: A. Counting viable cells B.…

A: Use the general formula: [Number of colonies counted] × 10 × [how many times the sample must be…

Q: Is nutrient agar (NA)  a complex or defined medium? Explained based on its composition. Is NA a…

A: a complex media is a media in which the exact composition is not known . it usually conatins…

Q: Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows…

A: Introduction: The given flux equation depends on the concentration of the retentate, and other…

Q: (b) (c) Explain the biological requirements for microalgal growth and the main system components for…

A: Microalgal production systems are systems that use algae to produce commercial products such as…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.