What is the reason why using a spectrophotometer is considered an indirect measurement of cell density?

Q: Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations…

A: Density gradient centrifugation is a common technique for isolating and purifying biomolecules and…

Q: What is the major difference between animal cell culture and plant cell culture.

A: Cell culture methods used to cultivate cells in the laboratory include animal cell culture and plant…

Q: A cell suspension that is more turbid than another cell suspension (of the same cell type and media)…

A: Cell is the fundamental unit of life. It comprises of macronutrients such as carbohydrates,…

Q: How would light shone on one side of the column influence the growth of microorganisms?

A: Different microbes have different growth requirements.  It can be minerals , temperature,  ph ,…

Q: What terms are used to describe organisms whose growthpH optimum is very high? Very low?

A: In the living world, we see a large number of microorganisms, animals and plants. These organisms…

Q: How does the plant cell wall react when it is placed under hypotonic medium?

A: The plants are the multicellular eukaryotic organism of the kingdom Plantae. It is the autotrophic…

Q: What instrument is used to check the turbidity for a test tube? O Scale Desiccator Photospectrometer…

A: Question- What instrument is used to check the turbidity for a test tube?  Scale Desiccator…

Q: If you made a plant cell drawing and that drawing measured 100 mm, what is the plant cell's…

A: If a plant cell is measured 20 µm this means that, 0.02 mm.

Q: Which metric unit is most appropriate for expressing the size of bacterial cells? a). nanometer b).…

A: In science standards unit of measurement is metric. Some organisms can be seen by naked eyes. Their…

Q: Use the counting method to estimate the length of an elodea cell. You will need to use your…

A: If the magnification is 400x then the diameter of one single cell would be .04mm  To find the length…

Q: The mud at the bottom of a Winogradsky column is usually found to have a black colour. Explain why?

A: Sergei invented the winogradsky column to view the enrichment of different microorganisms of various…

Q: Would heating the culture in a sealed, small-diameter tube that is totally immersed in a water bath…

A: Microbial culture is a process of multiplication of microbial organisms by allowing them to…

Q: Why do we use sucrose solution when we observe plant cells? What explanation

A: When a plant cell is placed in a sucrose solution i.e in hypertonic solution water will move from…

Q: You have a yeast cell stock in a flask. The total volume is 10mL. After counting and calculation,…

A: The square count method is done with a hematocytometer.

Q: Three-dimensional images of specimens can be produced using z stacks obtained from confocal…

A: Microscopy is a technique that is used to visualize small-sized objects that are not used to the…

Q: If I placed pieces of beetroot in a test tube and the test tube was placed in hot water, making the…

A: Beetroot contains a reddish purple color pigment which belongs to the chemical family called as…

Q: What are the problems related to the use of microalgae?

A: Algae are aquatic organisms. They can perform photosynthesis. Microalgae are a group of algae that…

Q: Explain the importance of using a control tube with only broth when measuring turbidity in a…

A: A spectrophotometer is used to measure the turbidity of a microbial culture sample. It is also used…

Q: How does a Winogradsky column show actual environmental conditions?

A: Winogradsky column is a device invented by Sergei Winogradsky in the 1880s that is used to culture…

Q: If a slide has 28 yeast cells in a field of view on 100x, approximately how many yeast cells will be…

A: When magnification increases 4 times that is from 100X to 400X, the field view decreases by 4 times.

Q: is there a correlation between one medium supporting growth at a wide set range or organismal growth…

A: A growth medium is designed to grow different types of cells. It is also known as culture medium and…

Q: What is the relationship of a solvent concentration to the penetration coefficient of cell membrane?

A: A solvent is anything that dissolves solute molecules in it. Water is also known as the universal…

Q: Briefly describe how magnetic bead microrheometry is used to measure the mechanical properties of…

A: A magnetic bead microrheometry  has been designed which allows the generation of forces up to 4.5…

Q: What are the advantages and disadvantages of viable cell count and the turbidimetric methods?

A: In turbidimetric determination of cell growth ,after inoculation of the bacterial culture the…

Q: what is the plant cell's magnification that measured 20 um under the microscope?

A: The given question is to find about the magnification of plant cell under the microscope.

Q: CELL COUNTING FOR DETERMINING VIABLE CELL CONCENTRATION Please give me a general introduction to…

A: The smallest structural and functional unit of life is the cell. It's just a piece of rudimentary…

Q: If sea fireflies (Cypridia hilgendorfii) are heated in water at 100oC for a few minutes before…

A: Question - If sea fireflies (Cypridia hilgendorfii) are heated in water at 100oC for a few minutes…

Q: The cell is usually placed after the monochromator, except in the case of using a multichannel…

A: A multi-channel analyzer is an instrument used in laboratory and field applications so to analyze an…

Q: From what you know of tonicity, what can you say about the plant cells and the solutions in the test…

A: Tonicity is the relative concentration of solutes dissolved in a solution that determines the…

Q: What do you mean by Auxanometer?

A: The process of plant development can be easily studied by the activity of the meristem. The…

Q: If you made a plant cell drawing and that drawing measured 100 mm, what is the plant cell's…

A: To calculate the magnification of a cell, the actual image size, and the image size. the divination…

Q: Which of the following methods used to enumerate cells often requires specialized staining to…

A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…

Q: Look at the slide . Count the number of cells in each quadrant. How many cells are in the sample,…

A: Hemocytometer is a device that was designed by the French anatomist Louis-Charles Malassez to count…

Q: All of the following are valid measurements of growth except: A. Counting viable cells B.…

A: Use the general formula: [Number of colonies counted] × 10 × [how many times the sample must be…

Q: when viewing an organism under the 40x objective lens, you estimate that 6 organims could fit across…

A: 40x objective lens are high powered objective lens which is used to observe fine details in a…

Q: In cell counting, why do you need to calculate for the volume of media needed after computing the…

A: The cell enumeration is important for seeding the appropriate cell density into the media flask.

Q: Define what is a minimal medium and the nature of its components.

A: A culture medium can be defined as solid,liquid or semi solid medium which helps in support of the…

Q: A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X…

A: A compound microscope is used by microbiologists to magnify microscopic samples. It uses a…

Q: A plant cell was isolated and submerged in a varying solution, given the below figure, how many…

A: When a plant cell is placed in hypotonic solution,it will get turgid(increase in size) and…

Q: Imagine a cell that truly has a Gram-positive style of cell wall. Which of the following staining…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: What happens to winogradsky column if it is put in a dark place in room temperature.

A: The Winogradsky column is a small, enclosed ecosystem used to enrich sediment microbial communities,…

Q: You used the stage micrometers to measure the FOV for your microscope at 400x total magnification at…

A: Given  Mag1 = 400x  FOV1 = 225 µm  Formula use = Mag1 × FOV1 = Mag2 × FOV2  Find  FOV at  1000x and…

Q: What is the principle of Grams staining based on the cell wall composition of bacterial cell.

A: Bacteria are a kind of organic cell. They comprise an enormous area of prokaryotic microorganisms.…

Q: The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is…

A: Microscopy is one of the basic processes on the basis of which the whole field of Biology rests.…

Q: Why is cell imaging a preferred methos for studying cells

A: Cell imaging a preferred method for studying cells because it enables the visualization and…

Q: The following image shows part of counting chamber you used to estimate cell viability for yeast…

A: For the calculation of cell viability:- 1. All the dead cells and the living cells are added for…

Q: What is resolution and how is it improved? With which objective is it permissible to use the course…

A: Using the fine adjustment knob which is inside the coarse adjustment knob and is used to bring the…

Q: The image above was taken using a 40x objective lens in our MoCell lab with 2.5 calibration factor.…

A: A microscope is an instrument that can be used to observe the small objects like, cells. The image…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."