CRISPR sequences are commonly found in O a. Prokaryotes; DNAse O b. Eukaryotes; Cas9 O Eukaryotes; DNAse O d. Prokaryotes; Hexokinase Oe. Prokaryotes; Cas9 and, when associated with an enzyme known as can serve to edit genes within an organism

Q: What is a genomic library? O collection of sequences made of DNA fragments O collection of vectors…

A: The molecule that contains the genetic information necessary for an organism's growth and…

Q: What would three possible reasons be that a PCR wouldn't work after cloning a TAQ sequence into a…

A: Even though you've verified Taq protein expression through SDS-PAGE, your PCR might still fail for a…

Q: Mapping high-throughput sequencing reads (choose all that apply) O requires a special device called…

A: Mapping high throughput sequencing refers to the amount of DNA molecules that read at the same time.…

Q: you carry out DNA editing using CRISPR whter the editing template DNA has strands labeled with heavy…

A: DNA (Deoxyribonucleic acid) is edited with the help of several molecular tools. CRISPR (Clustered…

Q: When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on…

A: Polymerase chain reaction (PCR) is a frequently used approach for rapidly producing millions or even…

Q: CRISPR-CAS9 is a: s a: alien weapon to control our minds a eukaryotic gene protein matrix type of…

A: CRISPR-Cas9, which is short for clustered regularly interspaced short palindromic repeats and…

Q: Suppose you have previously located and cloned a human gene on chromosome 2. You now believe there…

A: The genome of an organism is defined as the whole genetic information that is inherited from one…

Q: 6.c. Mutated DNA Template Strand #3: 3'-TACTGACTGACTATC-5' Complementary DNA sequence: mRNA sequence…

A: An explanation of the movement of genetic information within a biological system is the core tenet…

Q: PCR is used for cloning DNA and after about 30 cycles, the number of copies DNA will have increased…

A: The enzyme DNA polymerase which is used for PCR reactions is called Taq DNA polymerase which is…

Q: In biotechnology, an organism modified by gene splicing method is called Which of the following…

A:

Q: You have been given the DNA sequence for a particular fragment of DNA. You then isolated the mRNA…

A: Polymerase chain reaction is an artificial process that produces copies of a fragment of DNA and…

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Introduction The Process Of Transcribing A Piece Of DNA Into RNA Is Known As Transcription.…

Q: Explain with help of diagrams the mutagenic effects on DNA of following mutagens? Below is an…

A: Introduction: Mutation- These are the random heritable changes that occurs in the DNA sequence among…

Q: Match up the DNA mutation with its description: Silent a. a point mutation where one amino acid is…

A: Silent -  g) a point mutation where the amino acid sequence are unchanged Missense mutation -  a) a…

Q: automated sequencing is used are given a printout of the sense strand of your DNA.  The printout is…

A: Biomolecules are acknowledged as key components of electrical devices in DNA computing. This topic…

Q: 1. Describe the difference in the bands for the PCR and RT-PCR from the CD4+ T cells (ie. Make an…

A: PCR is a biochemical technique that is used for target-specific amplification of DNA. dsDNA is…

Q: WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger)…

A: The entirety of an organism's genetic code, or DNA, is known as its genome. It includes all the…

Q: Real-time PCR is used in many transcription? Select one or more: O a. Gene transcription qu b. Ob.…

A: Note- According to the guidelines only one question can be answered at a time. Hence, the first…

Q: The seocond part of this question is: how could you find a way to improve the metabolic efficiency…

A: We can take into account the various oxidative phosphorylation steps to pinpoint the precise…

Q: Given the Following DNA template, TAC   CGC   TCC   GCC  GTC   GAC   AAT   ACC   ACT, write out the…

A: The DNA template is used for the synthesis of mRNA molecules. mRNA molecule is used by the…

Q: Pair the appropriate item with its purpose in the production of insulin a DNA containing the gene…

A:

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: i have a gene (3222bp) i want to put it into a expression vector (pet 28b+),when i did pcr i flanked…

A: Introduction  Gene cloning is the technique to produce the exact copies of the desired gene by…

Q: 6. Below are several DNA sequences that are mutated compared with the wild-type sequence: 3'-TACT…

A: Wild type sequence:                           3' TACTGACTGACGATC 5' Complementary : 5'…

Q: gene editing and RNA interference.

A: Gene editing:  It is defined as the type of genetic engineering in which DNA is inserted or…

Q: Mutations that eliminate the function of a protein are generally associated with:     missense…

A: According to guidelines we have to answer first question only. please kindly post the remaining…

Q: What is the natural purpose of restriction endonucleases in bacteria? O To digest the bacterial…

A: According to the question, we have to find out what is the natural purpose of restriction…

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: Supercoiling of DNA A requires GTP as source of energy B is not observed in eukaryotes (c) requires…

A: Requires the action of topoisomerases. Option-C

Q: Which of the following is a type of transposition that involves the movement of DNA within a genome…

A: DNA transposition is the movement of DNA sequence from one location to another. This movement is…

Q: During RT-PCR, a poly-T primer and reverse transcriptase is used to turn all the mRNA to DNA. Why is…

A: RT-PCR, or reverse transcription polymerase chain reaction, is a widely used technique for the…

Q: Primers are composed of and are made by the enzyme DNA; helicase DNA; primase O RNA; primase O RNA;…

A: A primer is a single strand of DNA that is short and acts as a starting point for the synthesis of a…

Q: Supercoiling of DNA    requires GTP as source of energy   is only observed in prokaryotes   is…

A: DNA is a genetic material present in most living organisms. In case of eukaryotic cells DNA is found…

Q: 10.  Why does a cDNA copy of a eukaryotic mRNA gene need to be made in order to determine the…

A: Introduction :- DNA acts as the genetic material of the cell . DNA is made up of two strands , and…

Q: The difference in genome size between prokaryotes and multicellular eukaryotes are mostly due to The…

A: The following is a question related to genetics and molecular biology that requires a basic…

Q: assess anatomical distribution of gene expression.   genomic pcr nortbern blotting Rnai in situ…

A: The genes are the functional part of the DNA that undergoes transcriptional process and ultimately…

Q: R plasmid R determinant CRISPR tracrRNA sgRNA E = A CRISPR nuclease that can cut foreign DNA and…

A: Molecular techniques are used to study the biological activities at a molecular level. These…

Q: Synthesis of hybrid genes can occu Directed mutagenesis O Using overlap primers O RT-PCR

A: PCR technique of amplification of a given molecule of DNA using taq polymerase enzyme.

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Transcription  It is a biological processe where RNA is made by using DNA as a template.

Q: Which of the following tools should be used in identifying a mutation in a sequence compared to a…

A: Gene mutations, and alterations in the DNA sequence, play a fundamental role in shaping life and…

Q: ween a DNA l

A: DNA Library : It is the collection of DNA sequences from different organisms. cDNA : It is the DNA…

Industrial Processes

Industrial processes result in the manufacturing of products (biological products, such as enzymes, medicine) on a large scale using different methods, such as physical and chemical procedures. Few products such as pharmaceuticals, agricultural products, food items, etc., involve microbes and their enzymes. Such processes are termed industrial biological processes. Microbiology and biotechnology enable us to utilize the ability of microorganisms to produce enzymes, primary metabolites, and secondary metabolites, which in turn help to produce the desired products.