10. Which area on a streak plate will contain the greatest amount of growth? The least amount of growth? Explain your answers. 11. What is a colony (as viewed on an agar plate)? How can a pure culture be obtained from a mixture of bacteria? 12. What is a selective agar? Why can it be of importance?

29.  Identify a FALSE statement from the following,  Group of answer choices bacterial growth is related to nutrient concentration and availability sulfur is essential in growth medium for some amino acid synthesis none of the above is an false statement nutrients uptake can be via active or passive process culture medium must be sterile before inoculation     30.  Identify a CORRECT statement regarding  penicillin allergy? Group of answer choices the hypersensitive reactions are mediated by IgE penicillin is degraded to penicilloyl and other breakdown products all of the statements are correct penicilloyl haptens trigger most allergic reactions anaphylactic shock is the immediate danger penicillin can provoke

Correct the answer

What is agar and what makes it the best agent for making culture media? explain

Which of the following terms are used to describe the appearance of the edge or margin of a colony growing on an agar surface? Check all that apply turbid entire undulate convex lobate umbonate

5. Give two reasons for why heat fixing is used?  Adherence of bacterial cells to the slide Denaturing of bacterial enzymes to preserve cellular integrity

Using a batch culture method, the effects of 3 types of media on the growth of a plant suspension culture was investigated. Figure below has shown the growth of Murashige and Skoog (MS), Gamborg B5, and Vacin and Went media in plant suspension cultures. Discuss on the findings and draw conclusions. With explanation, recommend which medium is best for maintaining suspension cultures

5. Using a pencil as your loop draw the streaking pattern used on a Petri plate to best achieve isolated colonies. How many times do you dip your loop into a broth culture? Number each streak in order.

You take two stainless steel pins and place each in a separate pan of boiling water for 15 minutes. Using sterile forceps, you then remove each pin from the boiling water and place each separately in a tube of sterile nutrient broth. You put the two tubes of broth in a 35-degree Celsius icubator for two days and then observe them for miucrobial growth. In one you observe extensive growth as evidenced by heavy turbidity, but the other tube remains completely clear, indicating that no growth occurred. Explain.

During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain.