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Complementation and mapping analyse of Lac− mutants
Introduction:
The lactose operon is a type of operon that is needed to transport and metabolize lactose in the prokaryotic cell including E.coli. Glucose is the primary source of carbon for most of the prokaryotic bacteria but in absence of glucose, the bacterial cell adapts itself and alter its metabolic machinery to metabolize galactose.
Step by step
Solved in 2 steps
- a. For the E.coli haploid genotype below, determine the phenotype for the following proteins. Assume that any genes or structural elements not shown are wildtype. lacls lacz lacY* lacz [ Select ] lacY [ Select ]C- PETT-1 PETT-2 It seems PETT-2 worked better than PETT-1. Examine each structure carefully. Provide plausible reasons for the differences in these compounds inhibiting their target enzyme, reverse transcriptase. How might you improve the efficacy of PETT-1 and PETT-2?flow chart of purification steps of Beta-fructofruanosidase (Invertase)
- A cytosine is deaminated. Describe the outcome of this deamination and explain in detail how E. coli repairs this mutation using base excision repair (BER). This repair should contain 5 steps and describe the function of all enzymes or structures.Molecular detail of D614G strain of SARS CoV-2Molecules of DNA Polymerase III per Cell vs. Growth Rate It is estimated that there are 40 molecules of DNA polymerase III per E. coli cell, is it likely that the growth rate of E. coli is limited by DNA polymerase III availability?
- Need help Define Bacteriocin, give a list of the different types of bacteriocins and the effects they have on gram negative and gram positive bacteriaThis is a missense mutation explain. Include the ideas of translation and transcription. Compare the normal and abnormal strands.expanded structure of the D-tagatose Fischer projection of D-tagatose Haworth projection of D-tagatose
- what is lactose intolerance ? describe the molecular life cycle for this disease. also describe how it occurs in a molecular level detailed mechanism. what causes this disease and how it develops ? provide detailed biochemical phenomena and life cycle for Lactose Intolerance condition.Translation in eukaryotes and prokaryotes are similar and yet different. From a therapeutic perspective, why is this advantageous?Micrograph of red blood cell and the amonia acid composition of the relevent portion of beta chain of heomoglobin?
