Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Briefly describe (in 6-7sentences) what a plasmid is, and how it can be used to transform bacteria like E. coli.
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- Part ii. ) pleasearrow_forwardYou utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using plasmid maps like the one below. This map shows the positions of recognition sites for a number of restriction enzymes Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzymes EcoRI and PvuII.arrow_forwardSequence the steps to transfect and observe a fluorescent protein in a slice of the brain: * Place the slice of brain under the microscope and observe it. * Wait for the expression of the fluorescent protein by the transfected cells. * Harvest and culture a slice of brain. * Electropore in the slice a plasmid coding for a fluorescent protein.arrow_forward
- Explain how DNA configuration affects the migration of cut and uncut preparations of a plasmid?arrow_forwarddescribe the two main parts of a plasmid DNA extraction and purification procedure including the purpose of each steparrow_forwardA.) Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another B.) What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on…arrow_forward
- Briefly describe what happens during each of the phases of PCR (denaturation, annealing, and extension), including when you need each of the major components of the reaction (template, primers, nucleotides, DNA polymerase).arrow_forwardBesides their role in genetic engineering, what else do plasmids can plasmids be used for? How are restriction enzymes used in genetic engineering?arrow_forwardDescribe the genetic makeup of a plasmid in an F’ cell, including the types and origins of genetic information contained in this plasmid, and the mechanism by which such a plasmid arises.arrow_forward
- You first need to create a plasmid. What are the minimal components necessary to develop this plasmid? In addition to these components, please draw the plasmid. In this illustration, I am looking for the components, the direction of transcription (i.e., the direction the genes will be transcribed), and what should be transcribed last? Also, where would you specifically insert the P. falciparum gene in this plasmid, and why are you checking reading frames in your gene? Finally, please name your plasmid using the correct nomenclature too. You are excited you have this new plasmid; you want to transfect it into P. marinus and provide it as an oral vaccine to laboratory mice. However, even though your supervisor enjoys your enthusiastic attitude, they do not allow you to do this quite yet because all you have is this plasmid. Why doesn’t your supervisor allow you to use the laboratory mouse for this research regarding animal welfare guidelines? Your answer should include the 3 R’s of…arrow_forwardPolymerase chain reaction cannot amplify complete circular plasmids. True Falsearrow_forwardAssume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.arrow_forward
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