Compare DNA polymerase and RNA polymerase from E. coli in regard to each of the following features: (a) activated precursors,(b) direction of chain elongation, (c) conservation of the template, and(d) need for a primer.

and set up a series of four dideoxy reactions (the dideoxy nucleotides are labeled with radioactive P32 ).    You then separate the products of the reactions by gel electrophoresis and obtain the following banding pattern:      a) Write out the base sequence of the newly synthesized strand from reading the gel (include the 5’ and 3’; end)  b) Write out the base sequence of the original fragment you were given (include the 5’ and 3’ end)

a. Propose three different mutations to prevent initiation, elongation, and termination of bacterial DNA replication, respectively. Explain how/why each mutation would prevent its respective step. (Hint: mutations can be in genes that encode proteins or regulatory DNA sequences) b. In the early 1900s, Avery, MacLeod, and McCarty performed an experiment in bacterial cells to determine whether DNA, RNA, or protein functions as the 'transforming molecule' (i.e. the genetic material). In your own words, how did their experiment (depicted in the figure below) help to answer that question?

Please label the following: (A) INITIATION *Complementary strand *Template Strand *RNA polymerase *Initiation Site *Termination Site *Promoter *5' and 3' end on each DNA strand Initiation (A) FRA FORÆTSIST Elongation (B) (C) restorag Termination IND PLATIN RNA (B) ELONGATION *Exiting DNA *Exiting RNA Transcript *Template Strand *Nucleotides (ATP, UTP, CTP, GTP) *Direction of transcription PHILLI 13' DIDI