Common bean (Phaseolus vulgaris L.) is a legume deficient of an essential amino acid methionine while wheat (Triticum aestivum) is rich in lysine. Common bean crop needs to be fortified for nutritional improvement using genetic engineering. What could be the best transformation method for transfer of that particular set of genes in to common bean genome. Elaborate some conventional and non-conventional methods that could be used

Q: what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the…

A: Whole - genome shotgun sequencing is a single step sequencing method, in which the genome is broken…

Q: Two homologous chromosomes in ES cells are depicted below with the portion of your gene of interest…

A: CRISPR/Cas9 creates specific double-stranded breaks at the target locus that trigger DNA repair…

Q: The human hexokinase enzyme has the same function as the bacterial hexokinase enzyme but is somewhat…

A: Promoters are upstream regions of a gene that are used to initiate and control gene expression. The…

Q: DNA from a strain of bacteria with genotype a+ b+ c+ d+ e+ was isolated and used to transform a…

A: Transformation is a process of genetic recombination in bacteria. It creates genetic variations in…

Q: What color is the alpha gene spot on the microarray? Answer: Yellow please explain why is the…

A: The microarray can be described as a lab-on-a-chip. It can be used to detect the expression of…

Q: List at least two ways in which computerized analysis oftranscriptomes aids genetic research.

A: Genetic research refers to the study of human deoxyribonucleic acid (DNA) to order to know the genes…

Q: The figure below shows RNA-Seq data (RED) for the D. melanogaster transformer (tra) gene obtained…

A: DNA is made up of nucleotides. DNA consists of exons which are the coding sequences and introns…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: Describe at least five (05) different types of commercially available vectors for cloning with size…

A: Cloning vectors are small DNA parts that are stably maintained in an organism. The foreign fragment…

Q: If a pair of 10-mer primers are used in a PCR, what would be the number of expected binding sites in…

A: PCR is a polymerase chain reaction, in this process DNA is amplified . For this amplification,…

Q: During your experiment you analysed only a few of the recombinant clones for the presence of the…

A: A Genomic library consists of the total genomic DNA of an organism. This DNA can be stored inside…

Q: List the three features of a cloning vector and why these elements are needed

A: Cloning vectors act as a vehicle to transfer desired foreign genetic material into a cell. All these…

Q: Each of the following describes a distinctive step in a genomic technology or an experimental…

A: Genomics is a branch of molecular biology that focuses on the study of genomes, which encompasses…

Q: What is a recombinant vector? How is a recombinant vector constructed? Explain how X-Gal is used in…

A: To transfer set of desired artificial or natural genes into target organisms employs the need of any…

Q: A method for detecting methylated CpGs involvesthe use of a chemical called bisulfite, which…

A: Hi! Since you have posted multiple questions, we are answering only first three sub-parts. Primers…

Q: A wild type strain of Drosophila was found to carry a transposable element called copia. From this…

A: The following is a short summary of the genetics terminology and Drosophila notation used in this…

Q: You are engineering a new vector that contains a screenable marker that can be used for blue/white…

A: Three important points to remember about the blue-white test: a special substrate is used which is…

Q: FOR PKU:  Draw what you’d expect to see if you were to analyze the gene products by conventional…

A: Analyzing PKU gene products: PCR, RT-PCR, and Western BlotPKU (Phenylketonuria) is an autosomal…

Q: Transgenic tobacco plants were obtained in which the vector Ti plasmid was designed to insert the…

A: A gene is a unit of hereditary present in thousands of numbers on the helical strands of…

Q: Consider the following types of cells: F+, F-, Hfr, and F’ cells. Which of these four types of cells…

A:

Q: Propose a method for isolating a DNA fragment that is adjacent in the genome to a previously…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: this is an example of biotechnology: In order to increase the yield of oil from canola, research…

A: Biotechnology is the branch of science that deals with the production of various products by using…

Q: Discuss the differences between transposable elements that are genomic parasites and transposable…

A: A transposon can be defined as the characteristic mobile genetic element. These elements can…

Q: The sequence at one end of one strand of the Drosophilatransposon Mariner is shown below (dots…

A: The genes are the sequence of nucleotides that are present on the chromosomes and encode for a…

Q: List other methods of reverse genetics available in S. cerevisiae, Drosophila, Arabidopsis, and…

A: Saccharomyces cerevisiae commonly known as the yeast  are micro organism with application in…

Q: Following a dye terminator DNA sequencing reaction using 2',3'-dideoxynucleotide triphosphates…

A: Dye terminator DNA sequencing is the modern version of Sanger sequencing. It uses…

Q: In a clinical context, doctors are evaluating a therapy for a new patient (say patient B) that they…

A: In the setting of assessing treatment for a modern patient (patient B) who may develop comparable…

Q: STEP BY STEP Exaplanation would be appreciated so I can understand for upcomming exam. In humans,…

A: Given that  GG =570 GT = 297 TT =33  Total population = 894

Q: What is a repetitive element in genomics?  What are the types of repetitive elements?  What is their…

A: The amount  of DNA present in a cell of a person is called as genome. DNA is made up of nucleotides…

Q: Researchers have identified a gene (FR) responsible for watermelon resistance to infection by Dacus…

A: Any abrupt changes in DNA or RNA nucleotides that may or may not be responsible for the changes in…

Q: ISSR is generally a dominant STS DNA marker. Nonetheless, with validated experimental evidence (e.g.…

A: Codominant marker are inherited by both the parents to next generation in equal proportion. When…

Q: In DNA-hybridization experiments on six species of plants in the genus Vicia, DNA was isolated from…

A: The genomic size of the bacteria is much smaller than the genomic size of eukaryotes. The DNA got…

Q: For a molecular cloning experiment using the bacterial strain E. coli K12 is used to insert the gene…

A: Recombinant DNA technology uses enzymes and a variety of laboratory procedures to modify and isolate…

Q: Why the sequence alignment is extremely important in designing a construct for developing a…

A: Sequence alignment It is defined as the way of arranging the sequences of DNA, RNA or protein to…

Q: Below is a portion of an exon from a gene that encodes protein Y in the genome of the plant…

A: Ans 1 :  Yes, there is a mutation in third codon when counting from 5/ to 3/ on template strand in…

Q: cterium-mediated plant transformation is a method used to create transgenic plants cterium…

A: Agrobacterium tumeifaciens is a bacteria that invades some plants at the site of wound. They…

Q: E. coli strains diploid for the lac region were constructed by introducing a plasmid carrying the…

A: ANSWER;- I- mutation- repressor is unable to bind to the operator region. If there is no other…

Q: here is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods,…

A: In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or…

Q: How can you assess dominance and mapping with only one mutant?

A: Dominant alleles are those which are expressed when present in heterozygous conditions.  The

Q: While comparative genomics is fundamentally the study of the differences between the genomes of…

A: Comparative genomics is the study of genetics and other elements of biology by comparing the genomes…

Q: F ′strains in E. coli are derived from Hfr strains. In some cases, these F ′strains show a high rate…

A: Conjugation is transfer of genes in bacteria.cell to cell contact by F-pilus or sex pilus allows the…

Q: occurring

A: The Department of agriculture regulates field testing of GM crops for research. The environmental…

Q: As the leading scientist in a biomedical science laboratory, it is up to you to give advice to your…

A: Polymerase chain reaction or PCR s a method to obtained large number of copies of a target DNA…

Q: Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small…

A: As per the central dogma of biology, the flow of genetic information is from DNA -> RNA ->…

Q: What is homologous recombination and where/when does it typically take place in an organism.…

A: Homologous recombination is a genetic exchange process that occurs between two similar or identical…

Q: Why is the technique used to clone mammals called somatic cellnuclear transfer?

A: A somatic cell is a cell that takes part in the composition of the body of an organism and divides…

Q: Bacterial glucuronidase converts a colorless substance called X-Gluc into a bright blue indigo…

A: Bacteria‘s are prokaryotic unicellular organisms. Bacterial have incipient nucleus. E.coli is a gram…