Chemistryhelp Isoelectric Point ProblemA mixture of two proteins are to be separated by isoelectric focusingIsoelectric MolecularPoint (pl) WeightG3P Dehydrogenase (GDH)8.336.0DNA Ligase (Lig)6.060.0Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.In which direction will each protein in the table migrate at pH 7.0ProteinNameAA. Both GDH and Lig will have a negative (-) charge and travel towards the positive (+) terminalOB. Both GDH and Lig will have a positive (+) charge and travel towards the negative (-) terminalOC. GDH will have a positive (+) charge and travel towards the negative (-) terminalLig will have a negative (-) charge and travel towards the positive (+) terminalOD. GDH will have a negative (-) charge and travel towards the positive (+) terminalLig will have a positive (+) charge and travel towards the negative (-) terminal

pI (isoelectric point) is the PH at which a molecule carries no net charge. Isoelectric focusing is…

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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