A sample mixture consists of three proteins with the following properties: MW (kDa) 200 Amino acid composition 40% nonpolar, 60% polar 20% nonpolar, 80% polar 45 98 85% nonpolar, 15% polar Protein A B C IPH 9 3 5 1. If the mixture is subjected to ammonium sulfate precipitation, which protein will precipitate out first? [Select] 2. If the mixture is subjected to isoelectric focusing, which protein will stop moving nearest to the positive electrode? [Select] 3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH 7, which protein will bind to the resin? [Select] 4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost portion of the gel? [Select]
A sample mixture consists of three proteins with the following properties: MW (kDa) 200 Amino acid composition 40% nonpolar, 60% polar 20% nonpolar, 80% polar 45 98 85% nonpolar, 15% polar Protein A B C IPH 9 3 5 1. If the mixture is subjected to ammonium sulfate precipitation, which protein will precipitate out first? [Select] 2. If the mixture is subjected to isoelectric focusing, which protein will stop moving nearest to the positive electrode? [Select] 3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH 7, which protein will bind to the resin? [Select] 4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost portion of the gel? [Select]
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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![A sample mixture consists of three proteins with the following properties:
Protein
A
MW (kDa)
200
Amino acid composition
40% nonpolar, 60% polar
B
45
20% nonpolar, 80% polar
C
98
85% nonpolar, 15% polar
IpH
9
3
5
1. If the mixture is subjected to ammonium sulfate precipitation, which protein will precipitate out first? [Select]
2. If the mixture is subjected to isoelectric focusing, which protein will stop moving nearest to the positive electrode? [Select]
3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH 7, which protein will bind to the resin? [Select]
4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost portion of the gel? [Select]
* Previous
Nex](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F6bf53208-7c31-4c0d-b705-6bbe301cbf71%2Fa16faee0-2c39-4e13-90dd-844c7012cff5%2F6fquzt_processed.jpeg&w=3840&q=75)
Transcribed Image Text:A sample mixture consists of three proteins with the following properties:
Protein
A
MW (kDa)
200
Amino acid composition
40% nonpolar, 60% polar
B
45
20% nonpolar, 80% polar
C
98
85% nonpolar, 15% polar
IpH
9
3
5
1. If the mixture is subjected to ammonium sulfate precipitation, which protein will precipitate out first? [Select]
2. If the mixture is subjected to isoelectric focusing, which protein will stop moving nearest to the positive electrode? [Select]
3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH 7, which protein will bind to the resin? [Select]
4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost portion of the gel? [Select]
* Previous
Nex
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