2. What sample shows a positive result with the Molisch Test? Why did this sample give a positive result?
Q: The glycerol phosphate shuttle moves reducing equivalents (electrons) from the cytosol to the…
A: Shuttle systems are the systems which help in the transport of electrons from NADH present in the…
Q: Describe the process of denaturation of protein.
A: The proteins are most active in their native structure. Native conformation of the protein is the…
Q: DNA: Whats the structural and functional differences between heterochromatin and euchromatin?
A: DNA, the genetic material is organized as chromosomes inside the cell nucleus. The chromosomes have…
Q: The side chain of which residue can be hydrogen bond donor: a. Thr b. Ala c. Ile d. Pro e.…
A: There are twenty standard amino acids that makeup all the proteins present in our body. These twenty…
Q: For the structure shown on the figure - qualitatively draw Ramachandran plot. Assume a mixture of…
A: Ramachandran plot is plot used to visualize energetically allowed regions for the peptide backbone…
Q: What are the similarities and differences of intermolecular interactions that stabilize secondary…
A: As per the central dogma of molecular biology, DNA contains the code that is necessary for the…
Q: This question is about "ANSERINE" not just "SERINE
A: Anserine is a dipeptide composed of beta alanine and methyl histidine. It is found in brain of birds…
Q: In order to study protein structures and functions, many protein techniques have been developed for…
A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…
Q: a. The importance of recombinant DNA technology in Environment b. Potential products…
A: DNA : Deoxyribo nucleic acid. It is a polymer which is composed of two polynucleotide chains that…
Q: HN 1. 2. 3. 4. 5. NH CH₂ EB HẠN–ệ—C H 8 Choose A if the statement is CORRECT B if the statement is…
A:
Q: 10. Which of the following statements regarding insulin is false? A. Insulin is a signal that…
A: When blood glucose concentration increases or decreases, carbohydrate metabolism is coordinated by…
Q: Enumerate the 4 protein structures and give its corresponding process and location in the cell.
A: A protein is a polymer of amino acids. Protein can assume multiple conformations based on the nature…
Q: referred to feedback inhibition as “Goldilocks regulation”. Describe how feedback inhibition keeps…
A: Biochemical pathways and reactions are occurring constantly in cells. These pathways convert the…
Q: The overall process of glycolysis converts a C6 molecule into: O A different C6 molecule O Three C2…
A: Glycolysis is the catabolic pathway and is the first step of cellular respiration that takes place…
Q: is the statement true or false?
A: The pentose phosphate pathway carries out the complete oxidation of glucose by completely oxidizing…
Q: What is the product of the oxidation of this fatty acid?
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbon to 36 carbons.…
Q: In the absence of oxygen is glucose the major source of ATP?
A: It is generally agreed upon that ATP serves as the mediator of energy transfer between anabolism and…
Q: Assumed that you have isolated the protein cytochrome C from a cell lysate. Discuss the advantages…
A: Cytochrome C Cytochrome C is a protein that is loosely bound to the inner membrane of mitochondria…
Q: Consider the oligopeptide whose structure is shown below: How many peptide bonds are there? Give…
A: please note that we are supposed to write only first 3 sub parts of a question. We request you to…
Q: What is the meaning of "in vitro"?
A: Biochemistry is an attempt at explaining the living cell in chemical terms. A living cell is the…
Q: The active site _______________. a. is the compound that an enzyme reacts with during the chemical…
A: The active site is that region of an enzyme where substrate molecules bind. The binding of substrate…
Q: what are the chemical methods for glucose determination and their clinical significance?
A: Glucose is the most important carbohydrate fuel of the body. The normal concentration of glucose in…
Q: In bacteria, one of the main functions of pilli is A. Buoyancy B. Conjugation (genetic exchange) C.…
A: Based on nutritional mode, bacteria can be classified as autotrophic bacteria (those that can…
Q: What is the percentage (%) of glutamate that is protonated at pH 6.0? pKa of glutamate R-group is…
A: Amino acids are biomolecules in which an amino group and a carboxyl group are linked to the same…
Q: Draw the Biosynthesis of fatty acids from Acetyl CoA pathway and identify the different types of…
A: The biosynthesis of fatty acids from the acetyl-CoA occurs in the cytosol. It involves the addition…
Q: 24) Determine the fraction of Vmax that would be found at a substrate concentration of ½ Km, 2 Km…
A: As per Michaelis Menten equation, V = Vmax*S/(Km + S) Here, V is velocity Vmax is maximum velocity S…
Q: Draw the Gluconeogenesis pathway (pyruvate to glucose) and identify at most 5 different organic…
A: Gluconeogenesis is a metabolic pathway in which glucose is synthesized from certain non-carbohydrate…
Q: The starting substrate and active site of a Type I topoisomerase is shown below. During this…
A: Topoisomerases change the topology of DNA by breaking either one or two of the DNA strands and allow…
Q: For the amino acid arginine: Draw its complete protonic equilibria. Indicate the net charge of each…
A: Zwitterionic form is a form of molecule which has a net charge of zero.Also this form is very…
Q: 15. Cellular proteins are oftentimes post-translationally modified. Choose one of the following…
A: Post translational modification are the modifications done in the protein by covalently attaching…
Q: Give at least 10 functions of protein and describe each function. Cite an example for each function…
A: Proteins are the bio molecules which are very diverse in their structure and function. There are…
Q: 1. What happens to the pyruvate made during glycolysis under anaerobic conditions? Is this a redox…
A: Glycolysis is the breakdown of one molecule of glucose (6C) into two molecules of pyruvate (2 x 3C)…
Q: Fetal hemoglobin binds 2,3 BPG with greater affinity than adult hemoglobin. True False
A: Growing fetuses' receive oxygen and other nutrients from their moms. Fetal hemoglobin is the…
Q: DNA Protein Wild-type Gene GCCGAA Ala Ala-CH₂ Glu Ser GM AGC What is the subject of the figure…
A: Gene is the functional basic unit of heredity, composed of DNA. There are various functions genes as…
Q: Why do you think the TCA critical pathway in Metabolism is needed
A: Introduction There are various metabolic process occur in our body. all metabolic processes are…
Q: Draw the Conversion of Pyruvate to Acetyl CoA pathway and identify the different types of organic…
A: Pyruvate is formed from glucose during glycolysis. Conversion of pyruvate to acetyl CoA takes place…
Q: 4. What effect do the presence of other materials in a mixture have on the Rf of a specific…
A: Chromatography is a biochemical technique that is used to separate a mixture of substances. In this…
Q: Since myoglobin is red and it may interfere with the absorbance of Bradford Protein Assay at…
A: Bradford protein assay is performed to measure the amount of protein in an unknown sample. Coomassie…
Q: 4 parts Which refers to A. breaking something down in the body. B.Building something in the body?…
A: In human body, numerous reactions goes on. This is called metabolism. Metabolism results from…
Q: A pharmaceutical company developed a drug, CP788, that inhibits the interaction of Grb2 with SH2…
A: Grb2 ( growth factor receptor bound protein 2) is an adaptor protein whose function is to bring…
Q: Procedure 1. Place 2mL of milk in each of the 3 test tubes. 2. Heat one of the test tubes to boiling…
A: Test tube 1 contains once boiled (then cooled) milk , formaldehyde and methylene blue . Test tube 2…
Q: Why does the Asp-His ion pair contribute more energy at pH 6.0 than at low or high pH? At pH 10.0,…
A: pKa is the pH at which a weak acid is 50% dissociated into proton and conjugate base. At pH below…
Q: Give 5 disaccharides, draw its chemical structures, structure of sugar 1, and structure of sugar 2.
A: When two monosaccharides are connected by a glycosidic bond, a disaccharide is formed. Disaccharides…
Q: The acetyl group shuttle transfers the acetyl group of acetyl-CoA, produced by oxidative…
A: Acetyl CoA is produced in mitochondria and cannot leave mitochondria so it combines with…
Q: Describe what you know about enzymes. Toxic hydrogen peroxide
A: Note: Hi! Thank you for the question. We are authorized to answer one question at a time. Since you…
Q: In affinity chromatography, which of the following strategies is used to elute a GST-POI fusion…
A: GST-POI stands for Glutathione-S-Transferase tagged Protein Of Interest. Glutathione is the…
Q: Identify the different functions of the genes coded in the lac operon region.
A: Operon is a set of linked genes that codes for proteins that are functionally related. lac operon is…
Q: Using a biochemical map and text describe the process by which lactate is recycled from the muscle…
A: A soccer match involves rigorous exercise, The intense exercise requires a high amount of energy.…
Q: Consider the structure of the tripeptide below. H H₂N-C-C- 0=6 CH₂ CH₂ C=0 1 NH₂ pH 5: O pH 10: H O…
A: The given peptide has three amino acids joined by the peptide bond. There are three ionizable groups…
Q: Which of the following statements concerning glycolysis is correct? O The pathway for the process is…
A: The first process in breaking down glucose to release energy for cellular metabolism is called…
Step by step
Solved in 2 steps
- 1. Give the importance and purpose of the Cetrimide Test. 2. being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?1. write the equation for the reaction of the carbohydrate test with betadine.2. write down the equation for the protein test reaction with indicators of vinegar, salt3. write down the lipid correction equation with ingredients (celery, papaya, milk, hazelnut, carrots, margarine, oil, coconut milk, water)
- 1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutes1. Give the importance and purpose of the Cetrimide Test. 2. Discuss briefly how each of the different oxidase method is being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.Answer TRUE OR FALSE only. 1. All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium. 2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria. 3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure. 4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms. 5. Label all reagents with its name, concentration and date of its preparation except for water.
- Suggest one reason why syringes are used in vitamin C content of food anddrink investigation rather than burettes. THE ANSWER HAVE TO BE AS SHORT AS POSSIBLE!2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.1. Conclude regarding the chemical composition of the formation of film in fresh milk in biuret test and acrolein test.
- 1. What is the role of the substrate? Is it really necessary to add substrate in ELISA?Explain why, or why not.2. What is the label used in ELISA? Can we use different label? Justify your answer.3. Explain elaborately the principle involved in ELISA.4. What are the different applications of ELISA? 5. Explain the positive and negative results of ELISA.6. Why do you think it is called indirect ELISA?7. What are the different solid phases that antibody or antigen can bind to,in ELISA?8. What are the different materials needed in the experiment?9. What is theimportance of washing? When does washing is performed?10. Assuming that these are the 12 microplates. What is wrong with the result of the test? What do you think the cause of this occurrence? What should the medical technologist do?Tube Observation Inference 0.1000 M glucose Red precipitate 0.0500 M glucose Red precipitate 0.0250 M glucose Red precipitate 0.0100 M glucose Green precipitate 0.0010 M glucose Green precipitate 0.0001 M glucose Blue solution 0.1000 M lactose Red precipitate 0.1000 M sucrose Blue solution 0.1000 M fructose Red precipitate Urine 1 Green solution Urine 2 Orange precipitate Table 3. Results of Benedicts Test on different samplesDraw the steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.