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Complete Textbook Of Phlebotomy

Complete Textbook Of Phlebotomy

5th Edition

ISBN:9781337464314

Author:Hoeltke

Publisher:Hoeltke

Chapter10: Sample Preparation And Handling

Section: Chapter Questions

Problem 4RQ

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Question

Can you please help me to make this table profesional, I need two table the other which will be table 2 with trend line equation and R2 value please and thank you

(mm)
4
Din solution.
F. Analyzing the Gel
1. Pour destain into waste container. Add approximately 200mL of water to gel box. Gel may
curl up for a few minutes but will eventually become flat again.
2. Place plastic wrap on the surface of the light box. Carefully transfer your gel to the plastic
wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel
with a layer of plastic wrap.
3. Measure the distance travelled by the dye front. DYE FRONT =
54
mm
4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in
your ladder. Measure from the bottom of the well to each band.
5. Use the Excel Tutorial Video on graphing and solving for the KD sizes of the unknowns.
Protein Ladder (for trendline graph):
Migration
Rf=
Distance
Band Migration
Dye Front Migration
0-074
17
22.
Gently rock gel for one or two hours.
1010 Pemove excess stain.
28
31
Fi
0-111
0-166
0.203
0.314
0-407
esis
2.stup
0.759,
Molecular Weight
(in kD)
260 250.
75
站
20
15
Results
Disclusion Analys
Conclussion or Reface.
10
kDa
--260
--140-
--100-
-70-
-50.
-40.
-35-
-25-
-15-
--10
Log of
Molecular Weight
2.415
217
2
1.87
+7
1.57
1.4
1.3
1.17

2
Table 2
INCLUDE YOUR TRENDLINE, EQUATION, and R2 VALUE
Label
Y-axis
Body*
72
W
#3
E
Graph here
Label x-axis
A
$
4
R
B
%
5
I
MacBoc
T
U
6

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