BIOCHEMISTRY QUESTIONThe initial rate (VO) data as a function of substrate concentration [S] for an enzyme (Enzyme1) thatobeys Michaelis-Menten kinetics are shown in the table below. The totalenzyme concentration is 2.5 nM.[S](μm)16.08.04.02.01.0v (μMsec ¹)48.532.719.811.15.88(a) Calculate KM for the enzyme, using the estimated slope (slope = (y2-y1)/(x2-x1)) from aLineweaver-Burk plot. Show your work and don't forget the units!(b) When the enzyme concentration is 2.5 nM, a Lineweaver-Burk plot of this data gives a line with a y-intercept of 0.0106 μ M-1 sec. Calculate kcat for the enzyme. (Show yourwork and don't forget to include units).(c) A second enzyme (Enzyme2) also obeys Michaelis-Menten kinetics. Its kcat and KM are 800 sec-1and 5 µM, respectively. Which is the more efficient enzyme? Briefly explaind) Is Enzyme 1 diffusion limited? Explain.

Enzymes kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction…

Answered: The initial rate (v0) data as a…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

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An enzyme-catalyzed reaction has a KM of 20.0 mmol L-1 and Vmax of 17.0 pmol s-1. When a mixed inhibitor is added, the apparent KM is 50.0 mmol L-1 and the apparent Vmax is 5.20 pmol s-1. Calculate α.
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Consider an enzyme that follows standard Michaelis-Menten kinetics and has the following kinetic constants: %3| k2 = 1.5 x 10? s1 Еo 3D 1 х 104 М = 1 x 104 M a. What is the value of the maximum rate VM? b. Prepare a hand-drawn quantitative plot on graph paper (not a simple sketch nor an EXCEL-generated graph) of the enzymatic reaction rate versus substrate concentration (like Fig 3-3) using the kinetic parameters given above. Be sure to label the axes and include numeric values on the axes. C. Based upon your hand drawn saturation plot, at what substrate concentration is the enzymatic reaction rate 75% of Vm?
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The initial rate (v0) data as a function of substrate concentration [S] for an enzyme (Enzyme1) that obeys Michaelis-Menten kinetics are shown in the table below. The total enzyme concentration is 2.5 nM.