Calculate the partial pressure of nitrogen (in mmHg) that would be required. Part 1.4 In planning for this experiment, you have to order gas cylinders ahead of time. Which cylinder should you order the most of (nitrogen, oxygen, or carbon dioxide)? In other words, which gas will the experiment consume the most of?
You are a PhD student interested in studying the effects of hypoxia on the behavior of mesenchymal stem
cells. You want to culture cells in a hypoxic environment. You decide to use a tri-gas incubator, which allows
for precise control of the oxygen tension, as well as the partial pressures of nitrogen and carbon dioxide in
the cell culture system.
A tri-gas incubator is a type of cell culture system that allows for the control of the three main gases
in the culture environment: oxygen, carbon dioxide, and nitrogen. The oxygen tension and carbon diox-
ide tension in the culture environment can be controlled by adjusting the percentages of these gases in the
gas mixture that is supplied to the incubator. Hint: To calculate the partial pressure of nitrogen, you can use
pN2= pTotal −pO2−pCO2
Part 1.3
Calculate the partial pressure of nitrogen (in mmHg) that would be required.
Part 1.4
In planning for this experiment, you have to order gas cylinders ahead of time. Which cylinder should you
order the most of (nitrogen, oxygen, or carbon dioxide)? In other words, which gas will the experiment
consume the most of?
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