QUESTION 22 Mst digeston Hb*I Hb HDI HD Hb*I Hb E 1.35 kb E 1.16 kb Southern blot The figure above is a diagram of a Southern blot using a probe for the hemoglobin Hb gene. The blot shows a restriction fragment polymorphism (RFLP) related to the sickling trait (Hbs) in an Mst II restriction enzyme digest. Whích of the following statements about the location of the probe is correct? O A The Hbs mutation destroys an Mst II restriction site located within the probe hybridization region. O B. The Hbs mutation creates a new Mst II restriction site located within the probe hybridization region. O C. The probe hybridization region is completely contained on a 0.2 kb MstII restriction fragment. O D. The probe hybridization region is completely contained on a 1.15 kb MstII restriction fragment. O E. None of the above is correct.

QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…

QUESTION 22 Choose all the experiment(s) from the list below that demonstrated that RNA is involved in a transfer step between DNA and protein. O A. pulse chase experiments using radioactive deoxyribose O B. Brenner and Crick's characterization of frameshift suppressor mutations O C. analysis of the burst of RNA synthesis after bacteriophage infection of E. coli O D. pulse chase experiments using radioactive uracil bases O E. the Meselson Stahl experiment

Question 18 Match Column A with Column B. transports amino acids to the site of protein synthesis A. operator B. rRNA has anticodon loop C. IRNA v directs amino acid sequence of proteins D. MRNA contains the Shine-Delgarno sequence E. most type of RNA v synthesized in the eukaryote nucleus F. promoter v found inside the ribosome v region where the RNA binds to the DNA template region where the activator protein binds to the DNA template

Question 44 The tri snRNP particle O contains the snRNPs U4,U5 and U6 comes after the binding of U2 brings the branch point and the 5' splice site together O all the above A Click Submit to complete this assessment. Mac DO00

Question 4. You are analyzing cells that you have grown on coverslips and fixed. What method would you use to determine where in the cell a protein is located? A. Western blot B. SDS-PAGE C. Microarray D. Immnocytochemstry E. Single cell RNA sequencing

QUESTION 20 Match the lettered activities to the following enzymes. Remember that some enzymes have multiple activities. A. 5' to 3' polymerase activity B. 3' to 5' DNA polymerase activity C. RNA-dependent DNA polymerase activity D. DNA dependent DNA polymerase activity E. 3' to 5' exonuclease activity F. 5' to 3' exonuclease activity DNA polymerase II DNA polymerase I telomerase

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QUESTION 5 Based on the plasmid shown, what will you have to use to to distinguish and isolate the bacteria that successfully took up the plasmid? O Lactose O Tetracycline O Ampicillin O UV light to identify the glowing colonies O Xgal

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