Homework Help is Here – Start Your Trial Now!
Science
Biology
Why would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA?

Why would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

question 4

st your set of assigned enzymes and the expected fragment sizes here. Base Pairs 1. Using the gel picture to the left, show where your digestion of the paper 1,000 - 900 - cutting model showing cut fragments would appear. The linear uncut DNA is 640 base pairs in length. 800 - 700 - 600 - 500 - 400 - 2. Explain why the restriction endonucleases BglII (A^GATCT) and Xbal (T^CTAGA) will not recognize and cut the restriction site of the other. 300 - 200 - 100 - 3. What are the last three of six nucleotides (bases) of the palindromic site for 50 - the enzyme Ndel if the first three are C AT ? 4. Why would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA? 5. If the hypothetical restriction endonuclease CutVI were to recognize and cleave a circular piece of DNA at 6 different sites, how many fragments of DNA would result? 6. Answer the above question for CutVI if the starting DNA were linear instead of circular. 7. If the electrical leads were reversed by mistake (red connected to black), what would be the result? The gel would run backwards, with DNA moving the other direction. The DNA would separate as usual, only now the larger pieces would travel farther and smaller pieces shorter distances. The DNA would not move at all. A. В. С. 8. Explain your answer choice for the previous question in rational terms. DNA ladder Paper DNA digest
Transcribed Image Text:st your set of assigned enzymes and the expected fragment sizes here. Base Pairs 1. Using the gel picture to the left, show where your digestion of the paper 1,000 - 900 - cutting model showing cut fragments would appear. The linear uncut DNA is 640 base pairs in length. 800 - 700 - 600 - 500 - 400 - 2. Explain why the restriction endonucleases BglII (A^GATCT) and Xbal (T^CTAGA) will not recognize and cut the restriction site of the other. 300 - 200 - 100 - 3. What are the last three of six nucleotides (bases) of the palindromic site for 50 - the enzyme Ndel if the first three are C AT ? 4. Why would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA? 5. If the hypothetical restriction endonuclease CutVI were to recognize and cleave a circular piece of DNA at 6 different sites, how many fragments of DNA would result? 6. Answer the above question for CutVI if the starting DNA were linear instead of circular. 7. If the electrical leads were reversed by mistake (red connected to black), what would be the result? The gel would run backwards, with DNA moving the other direction. The DNA would separate as usual, only now the larger pieces would travel farther and smaller pieces shorter distances. The DNA would not move at all. A. В. С. 8. Explain your answer choice for the previous question in rational terms. DNA ladder Paper DNA digest
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Types of communication
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS