You can introduce precise edits into the genome using Crispr/Cas9 and , O A. ligation O B. homology directed repair OC.T-DNA transformation O D. CpG methylation O E. nonhomologous end joining

An EMS mutagenesis screen identified several mutants in this gene. The figure shows the position of two of these mutations a and b. The ucleotides are altered in these 2 different swo-1 mutant alleles. Use the genetic table to describe any AA changes.  Name the type of mutation and describe its effect on swo-1 mRNA and protein for each of the mutations 5. The swo-1 a mutation leads to worms with more body wall muscle, whereas worms with the swo-1 b mutation are not able to move. Based on these phenotypes and the findings from questions 3 and 4, describe the role thewild-type version of this protein plays in muscle function.

How might Salmonella contamination of food productionanimals be contained?

....... assess anatomical distribution of gene expression.   genomic pcr nortbern blotting Rnai in situ hybridization

1. What makes CRISP particularly useful compared to other genetic engineering techniques? A. CRISPR can add DNA to multiple cells at the same time B. CRISPR works better in bacteria C. CRISPR can target one specific area of DNA to cut D. CRISP is capable of transporting DNA through the cell walls of plants   2. MOST viruses are capable of infecting A. many different species B. many cell types in the same species C. only specific cell types that have a particular receptor

2. In order to make a recombinant plasmid for transformation, ЕcoRI you digested a bacterial plasmid with restriction enzyme 5'... GAATTC...3' EcoRI. You want to insert a gene of interest that codes for antibiotic resistance. You decide to try both XXRI and ZZRI 3... CTTAAG...5' restrictions enzymes for the antibiotic resistance gene. For XXRI, would you expect the gene insertion process to work? Why or why not? If you grow your transformed bacteria (assuming all other parts of а. XXRI 5... GAATTC...3 3..... СТТАA G...5 transformation work), what would you expect to see on an LB/antibiotic plate? b. For ZZRI, would you expect the gene insertion process to work? Why or why not? If you grow your ZZRI transformed bacteria (assuming all other parts of transformation work), what would you expect to see on an LB/antibiotic plate? 5... A A ATTL...3' 3... TTTA A A... 5

To quantify the transcription product of a specific gene in E. coli, you may use (select all correct answers) O a. Northern blotting O b. Topoisomerase I O C. Primer extension O d. Foot-printing

Based on recent data arising from various animal models, which of the following CRISPR-Cas9 systems would be the most promising to treat anxiety in humans? O A. CRISPR-dCas9 alone. O B. CRISPR-nCas9 fused with DNMT3A C. CRISPR-dCas9 fused with histone acetyltransferase. O D. CRISPR-nCas9 fused with deaminase and UGI. Reset Selection

8. You perform a PCR experiment using the template and primers shown below. What size is the amplicon (aka the PCR product) in bp? Template Q8: 5' TCGGC CGTAC TACGA TCGAT TGCGT ACGCA ATCGC -3' ¦¦¦¦¦ ¦¦¦¦¦ ¦¦¦¦¦ ¦|||| |||||||||| 3' AGCCG GCATG ATGCT AGCTA ACGCA TGCGT TAGCG -5' Primers: 5'-CGTAC-3' & 5'-TTGCG-3'

1. Drag the red box to indicate the restriction site in the following sequence. 5'-... АТА ТСА ТСС TGT GCT GCC T С АТC TСT сTG CCC ...-3' Attempts left: unlimited Check answer 2. Drag the green box to indicate the single base change or SNP in sequence 2. 1) 5-.. АТА ТСА ТСС TGT GCT GCС ТC АТC ТCT GTG CCС ...-3' 2) 5-.. АТА ТСА ТСС TGT GTT GCс ТтC АТС ТCT GTG CCС ...-3'