You can introduce precise edits into the genome using Crispr/Cas9 and , O A. ligation O B. homology directed repair OC.T-DNA transformation O D. CpG methylation O E. nonhomologous end joining

Q: Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS. You next take 0.5 mL of Dr.…

A: The first step is to calculate the average number of cells counted in the hemacytometer. The counts…

Q: Retrotransposons are mediated by the enzyme O a. transposase O b. RISC O c retrotransposase O d.…

A: Retrotransposons move by a copy and paste mechanism. However transportions describe the copy is made…

Q: Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? a.Single…

A: The game-changing CRISPR-Cas9 technology is changing the game of genome editing. The CRISPR-Cas9…

Q: When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on…

A: Polymerase chain reaction (PCR) is a frequently used approach for rapidly producing millions or even…

Q: Match Column A with Column B F. v Analysis of genome-wide patterns of gene expression F. v…

A: Recombinant DNA technology - It is a technology which forms the desired DNA sequence of interest by…

Q: Which of the following is an example of a recombinant DNA molecule? O a. a PCR-amplified…

A: Recombinant DNA technology : In this process two DNA molecules are attaches with each other for…

Q: Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end…

A: Base editing  It is an alternative tool to HDR-mediated replacement. This facilitates editing of…

Q: In 1996, a group of scientists from Scotland used a somatic cell of an adult sheep and fused it with…

A: Cloning

Q: The original function of CRISPR-Cas9 in bacteria is to: a. eliminate the function of any gene in…

A: CRISPR-Cas9 It refers to a gene editing tool that depends on the natural defense mechanism present…

Q: In biotechnology, an organism modified by gene splicing method is called Which of the following…

A:

Q: vnat Is the physiologic function of the CRISPR/Cas9 system? O It is used by bacteria to recognize…

A: CRISPR or "clustered regularly interspaced short palindromic repeats" are short viral origin DNA…

Q: PBR322 is an engineered plasmid and used in gene cloning procedures. Lütfen birini seçin: O a. True…

A: Gene cloning is process of synthesis of multiple copies of Genes . This process requires :- A ) Host…

Q: Which of the following sequences in a bacterium CRISPR locus will vary and change O a. Spacer DNA…

A: CRISPER is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria…

Q: The DNA site of a person diagnosed with Sickle Cell Anemia will be:

A: Sickle cell anemia is a genetic disease which occurs due to substitution of glutamic acid by…

Q: Which of the following approaches you can use to locate the relative positions of genes on…

A: Genes are located on chromosomes. These genes can not be targeted without using any specific gene…

Q: CRISPR sequences are commonly found in O a. Prokaryotes; DNAse O b. Eukaryotes; Cas9 O Eukaryotes;…

A: DNA is the genetic material of living organisms that contains necessary genes. Prokaryotic and…

Q: All are propertles of nucleosomes EXCEPT: / Almal is eienskappe van nukleosome, behalwe: A. all are…

A: Nucleosome They are fundamental repeating subunits of all eukaryotic chromatin (except when…

Q: In bact

A: Choose the correct answer.

Q: Pair the appropriate item with its purpose in the production of insulin a DNA containing the gene…

A:

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: Once you have idenified a gene that you suspect is responsible for the disease, you clone the gene…

A: Out of all the options given -  Option - Northern Blot , is incorrect because northern blotting is a…

Q: Match each word with the best description.…

A: As we know that Recombinant DNA technology is a set of techniques that enable the DNA from different…

Q: a. CRISPR-Cas9 is a valuable biotechnology tool. Explain how this system uses RNA and the result of…

A: CRISPR-Cas9  is known as unique biotechnology tool which allows medical researchers and geneticists…

Q: In organism that is mutant for components of the non-homologous end-joining NHEJ repair pathway…

A: CRISPR gene editing is a molecular biology genetic engineering tool for altering the genomes of…

Q: In T4 phage, the initial transcription is carried out by_ Select one: O a. the DNA polymerase…

A: ANSWER;- d) the RNA polymerase encoded by the T4 phage genome. Explain;- T4 phage, the initial…

Q: Using CRISPR/Cas9 to target a gene in the mouse will primarily result in: a. Any of the choices b.…

A: CRISPR/Cas9 is a powerful tool that can be used to target and edit genes. This technology has a wide…

Q: QUESTION 4 Which of these is not a component of the plasmid cloning vector O a. tetR O b. lacZ gene…

A: A vector is a tool to replicate foreign DNA into a host organism with the help of recombinant DNA…

Q: 1. Which of these methods will allow cell counting and segregation? a. Flow Cytometry b. Western…

A: Introduction :- Bio-physical techniques or methods are combination of biological and physical…

Q: make a mutation to disable the ACE2 gene (this gene encodes the protein that SARS-CoV-2 uses to…

A: We can edit genes with the technology known as CRISPR. In prokaryotic organisms such as bacteria and…

Q: Match the eukaryotic polymerases and accessory proteins with the polymerases (or polymerase…

A: PCNA stands for proliferating cell nuclear antigen. In prokaryotes three different types of DNA…

Q: Match the features of plasmids below with their meaning : Origin of replication LacZ gene antibiotic…

A: Plasmid is rounded Structure in the bacteria apart from main genome which functions as a a vehicle…

Q: If the F plasmid does not have the gene encoding Fino, this F plasmid would Select one: a. become…

A: Fertility factor or F factor or F plasmid is a sex factor that helps to transfer the genes between…

Q: 10) Scientists have engineered a simplified CRISPR-cas system for use in mammalian cells. What were…

A: The clustered regularly interspaced short palindromic repeats (CRISPR) is a technology that is used…

Q: The selection of the clones in plasmid-based directed mutagenesis depends on O a. Replication error…

A: NOTE: AS PER OUR GUIDELINES WE ARE ONLY SUPPOSED TO ATTEMPT THE FIRST QUESTION, PLEASE RESUBMIT THE…

Q: Eukaryotic RNA polymerase II is used to synthesize: a. rRNA O b. tRNA O C. O d. RNA from viral DNA O…

A: Transcription is the synthesis of RNA from DNA that is the process of copying the information of a…

Q: The CRISPR system may be used to manipulate the genome in human cells, because Cas9 can

A: CRISPER system can use Cas protein to manipulate the genome present in human cell.

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Transcription  It is a biological processe where RNA is made by using DNA as a template.

Q: a. A short segment of microRNA that becomes incorporated into DNA b. A segment of DNA that contains…

A: A gene is the basic physical and functional unit of heredity. Genes are made up of DNA.

Q: Based on recent data arising from various animal models, which of the following CRISPR-Cas9 systems…

A: based on recent data arising from various animal models, A. CRISPR-dCas9 systems would be the most…

Q: . Which of the following repair mechanisms can lead to frameshift mutations? a. Nonhomologous…

A: 1.Answer-- correct option is (b)        Nucleotide excision repair

Q: Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? A.Single…

A: Gene therapy has long been touted as a way to treat a wide range of human disorders and defects. The…

Q: Ester Lederberg's discovery of the lambda phage and F-plasmid were significant because: (select all…

A: Esther Lederberg, a microbiologist and a scientist who pioneered the field of bacterial genetics,…

Q: The 6th and 7th women Nobel Prize winners in Chemistry, Professors Jennifer Doudna and Emmanuelle…

A: The genome is a cell's whole collection of DNA blueprints. The genome of human beings is made up of…

Q: Attached is a cartoon model of the CRISPR system, with 3 critical components labeled.  A. What is…

A: CRISPR-Cas9 has recently become a popular set of tools for genetic engineering. By targeting…

Q: You are working for a pharmaceutical company are are tasked with creating E. Coli that express an…

A: Since you have posted a question with multiple sub-parts, we will solve the first three subparts for…

Q: Gene transcription quantification . In gene copy number detection in DNA genotyping 1 When…

A: It is a nuclear derived polymerase chain reaction method. It can detect any genetic material in the…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.