You can introduce precise edits into the genome using Crispr/Cas9 and , O A. ligation O B. homology directed repair OC.T-DNA transformation O D. CpG methylation O E. nonhomologous end joining

Q: What would three possible reasons be that a PCR wouldn't work after cloning a TAQ sequence into a…

A: Even though you've verified Taq protein expression through SDS-PAGE, your PCR might still fail for a…

Q: Retrotransposons are mediated by the enzyme O a. transposase O b. RISC O c retrotransposase O d.…

A: Retrotransposons move by a copy and paste mechanism. However transportions describe the copy is made…

Q: a bacterial cell in which the [ Choose ] F plasmid has become part of the chromosome lesion caused…

A: Given: To match all the given explanation with wording.

Q: Which temperature is Taq polymerase's optimal temperature O 20 degrees C 72 degrees C 55 degrees C O…

A:

Q: When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on…

A: Polymerase chain reaction (PCR) is a frequently used approach for rapidly producing millions or even…

Q: Match Column A with Column B F. v Analysis of genome-wide patterns of gene expression F. v…

A: Recombinant DNA technology - It is a technology which forms the desired DNA sequence of interest by…

Q: Which of the following is an example of a recombinant DNA molecule? O a. a PCR-amplified…

A: Recombinant DNA technology : In this process two DNA molecules are attaches with each other for…

Q: During retrotransposițion of a short interspersednuclear element (SINE), nicking the target site DNA…

A: A complete genetic material present in an organism is referred to as a genome. Deoxyribonucleic acid…

Q: Which of the following is FALSE about DNA methylation? O a. the base to be methylated is found in…

A: The CpG sites or CG sites are regions of DNA where a cytosine nucleotide is followed by the guanine…

Q: Which of the following are not the functional components of a simple retroviral genome?

A: All retroviral genomes include at least four genes, which are the gag, pro, pol, and env genes. The…

Q: Which of the following sequences in a bacterium CRISPR locus will vary and change O a. Spacer DNA…

A: CRISPER is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria…

Q: Explain with help of diagrams the mutagenic effects on DNA of following mutagens? Below is an…

A: Introduction: Mutation- These are the random heritable changes that occurs in the DNA sequence among…

Q: Which of the following will MOST LIKELY yield a construct containing an insert in the correct…

A: A vector construct, also known as a cloning vector or expression vector, is a DNA molecule that is…

Q: 15. The CRISPR/Cas9 system provides a form of adaptive immunity in bacteria. We discussed in class…

A: The correct option is DThe CRISPR/Cas9 system is a bacterial immune system to provide protection…

Q: The DNA site of a person diagnosed with Sickle Cell Anemia will be:

A: Sickle cell anemia is a genetic disease which occurs due to substitution of glutamic acid by…

Q: e CRISPR associated protein tinal

A: C) CRISPR is a tool of genome editing by using the endonuclease cas9, when the gene is cut by genome…

Q: 1. Describe the difference in the bands for the PCR and RT-PCR from the CD4+ T cells (ie. Make an…

A: PCR is a biochemical technique that is used for target-specific amplification of DNA. dsDNA is…

Q: Explain why H is 5' and E is 3'

A: DNA replication is the process of the copying of the DNA strand by the use of a DNA polymerase…

Q: Real-time PCR is used in many transcription? Select one or more: O a. Gene transcription qu b. Ob.…

A: Note- According to the guidelines only one question can be answered at a time. Hence, the first…

Q: however it is still unclear that the function of th gene is and the lab director asks you for…

A: Previously getting the idea of the genome of any organism was very difficult but, Recombinant DNA…

Q: What kinds of nonstructural genes are never translated

A: What kinds of nonstructural genes are never translated?   Answer. (b) transfer RNA and ribosomal…

Q: CRISPR sequences are commonly found in O a. Prokaryotes; DNAse O b. Eukaryotes; Cas9 O Eukaryotes;…

A: DNA is the genetic material of living organisms that contains necessary genes. Prokaryotic and…

Q: In bact

A: Choose the correct answer.

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: Once you have idenified a gene that you suspect is responsible for the disease, you clone the gene…

A: Out of all the options given -  Option - Northern Blot , is incorrect because northern blotting is a…

Q: Match each word with the best description.…

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Q: a. CRISPR-Cas9 is a valuable biotechnology tool. Explain how this system uses RNA and the result of…

A: CRISPR-Cas9  is known as unique biotechnology tool which allows medical researchers and geneticists…

Q: One problem that researchers sometimes encounterwhen editing genomes with CRISPR/Cas9 is that oneor…

A: The gene therapy or gene editing is an asset for humans, as many of the gene-related disorders can…

Q: In organism that is mutant for components of the non-homologous end-joining NHEJ repair pathway…

A: CRISPR gene editing is a molecular biology genetic engineering tool for altering the genomes of…

Q: In T4 phage, the initial transcription is carried out by_ Select one: O a. the DNA polymerase…

A: ANSWER;- d) the RNA polymerase encoded by the T4 phage genome. Explain;- T4 phage, the initial…

Q: 1. After exposure to a mutagen an A-T base pairing becomes T-A in the daughter cell. This is an…

A: Please note, keeping with the site regulations and in order to provide accurate and detailed…

Q: Please select all cellular processes that do NOT rely on RecA protein function as far as we…

A: RecA is a 38kDa recombinase protein found in prokaryotes and is essential for the maintenance,…

Q: What are potential uses of CRISPR-Cas9 Technologies? Silence genes to study their function Identify…

A: CRISPR-Cas9 is a gene editing tool that allows scientists to precisely target and cut specific DNA…

Q: Using CRISPR/Cas9 to target a gene in the mouse will primarily result in: a. Any of the choices b.…

A: CRISPR/Cas9 is a powerful tool that can be used to target and edit genes. This technology has a wide…

Q: 28. What type of mutation is seen here? WT: 5′-AUG GCU AGA GUU GAA AAA-3′…

A:

Q: "Knocking out" a gene with CRISPR-Cas9 leads to which of the following? O disruption of a gene O…

A: CRISPR-Cas9 is a unique technology that helps geneticists and researchers to edit a particular part…

Q: QUESTION 4 Which of these is not a component of the plasmid cloning vector O a. tetR O b. lacZ gene…

A: A vector is a tool to replicate foreign DNA into a host organism with the help of recombinant DNA…

Q: what is the Role of ATM in the cell and cancer ?

A: ATM gene which stands for Ataxia-Telangiectasis Mutated gene is a gene which is responsible for…

Q: Define the following terms: a. transfection b. replicative transposition c. composite transposition…

A: Note: Since you have posted a question with multiple subparts, we will solve the first three…

Q: 1. Which of these methods will allow cell counting and segregation? a. Flow Cytometry b. Western…

A: Introduction :- Bio-physical techniques or methods are combination of biological and physical…

Q: Which of the following is NOT a component of an IS element? O a. Target site direct repeats O b.…

A: Transposable elements are the sequence of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA)…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.