Biochemistry Question Two - Explain how gel filtration chromatography (size exclusion chromatography) separates molecules on the basis of their mass/size.

the 2 answers for both questions are right are right i just need the steps, i gave you 2 questions so you can double check your that the steps are right

9:54 AM Done Prelab 7 - Tagged (2 of 64) 三: Prelab 7 - Protein Catabolism In this lab we focus on another important group of metabolism processes: the breakdown of proteins and amino acids. In these labs we will utilize a series of media in tubes that differ in the protein for the bacteria to breakdown, and in the end products they can detect. There are a few new vocabulary terms. Proteolytic enzymes are enzymes that breakdown proteins, usually by first breaking apart the polypeptide into individual amino acids. Hydrolysis reaction is used to break apart the peptide bond holding the amino acids in the polypeptide together. Hydrolysis is used to breakdown urea, a compound produced by the body from the destruction of proteins. Once broken down to individual amino acid, often the amine (NH2) functional group is broken off one end in a deamination reaction, creating NH3. In a decarboxylation reaction the carboxyl group is removed, creating CO2. In a desulfuration reaction a sulfur is…

Which of the following correctly matches the chromatography technique with the molecular property being exploited for separation? ion-exchange: charge,affinity: polarity, gel-filtration: molecular mass ion-exchange: charge, affinity:molecular binding preference,gel-filtration: molecular size ion-exchange: charge,affinity: molecular binding preference,gel-filtration: isoilectric point ion-exchange: ion binding preference,affinity: molecular size,gel-filtration:polarity