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Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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have the following stock solutions:
10. Assume you sodium dodecyl sulphate (SDS)
10 %
V'S M NaOH
LTE
Perform the calculations and state how you would make 10 mL of lysis buffer, which has the
following composition in a single tube:
1% sodium dodecyl sulphate (SDS)
0.2 M NaOH
CIV2
X"
Transcribed Image Text:10:23
have the following stock solutions:
10. Assume you sodium dodecyl sulphate (SDS)
10 %
V'S M NaOH
LTE
Perform the calculations and state how you would make 10 mL of lysis buffer, which has the
following composition in a single tube:
1% sodium dodecyl sulphate (SDS)
0.2 M NaOH
CIV2
X
Expert Solution
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Step 1
Dilutions of any solutions can be calculated using the formula:
C1V1 = C2V2
Where,
C1= concentration of stock solution
V1= volume of stock solution needed
C2= concentration of final solution
V2= volume of final solution
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