1 2 3 4 5 6 N Sample Lysate Name volume 8 Tilapia Cod Sole Perch Tilapia Cod Sole 2 μl Perch 10 με 2 μ. 10 με 2 μl 10 μl [protein] [protein] in in assay lysate 3.9 µg/ml 19.9 µg/ml 18.2 µg/ml > 20 µg/ml 9.4 µg/ml 2 μι 10 με *assay range: 2-20 µg/ml What volume of the each fish "gel-ready" sample should you load on your gel to load 5µg of protein? > 20 µg/ml <2 µg/ml 7.3 µg/ml [protein] volume in to load 5 "gel- ready" μg sample protein 7.3 μl 4.2 μl μg protein in 20 μl 19.5 μl 4.2 μl > <

Test Description Terminal Terminal Medium, Incubation Incubation Results temperature time (°C) electron enzyme reagent ассeptor 1 Oxidase test Tryptic 24 – 48 hours soy agar (TSA), QxiDrop 2 Catalase Охудen test N/A Nitrate 37 reductase N/A test

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

Lab 2 MicrosCO X Copy of Lab 2 X M Inbox (53,887) x b Answered: 4. 1 x C Search Textbo X + /d/1lq1XGTjeDOK-AZLP5NCDYYPZT3K6_fPLnwx-GvHgLnU/edit# Normal text Arial 12 %00 L 1 2 3. 4. 5. 9. Observing Bacteria in Colonies In fresh or saltwater habitats, cyanobacteria will appear (without a microscope) as very small green lines (as can be seen in this video). Observe the following images of cyanobacteria colonies under 100x and 400x magnification with a light microscope: Anabaena sp. 100x magnification Anabaena sp. 400x magnification As you can see, cyanobacteria grow as two different types of cells: those that are photosynthetic (fixing carbon dioxide to make sugars), and heterocysts (that fix nitrogen to make proteins and nucleic acids). Although these cells are genetically identical, each is able to accomplish this via differences in gene expression, a key concept in biology you will continue exploring this quarter. In the 400x magnification image, you should be able to clearly see two…