Based on the structure of CBB G-250, explain why it can bind to proteins having basic and hydrophobic amino acid residues.
Q: Glucokinase (hexokinase IV) Hexokinase I, II, or III All hexokinase isozymes (I-IV) Answer Bank will...
A: Isozymes are the enzymes with same catalytic activity but differ in efficiency and enzyme forms. Hex...
Q: 7. Triacylglycerols are _____ in their ability to store energy when compared to glycogen, the energy...
A: “Since you have asked multiple question, we will solve the first question for you. If youwant any sp...
Q: Review Figures 8.12 and 8.13. In cells, the primers forDNA synthesis are short strands of RNA, so ea...
A: A primer molecule is a short strand of RNA. During replication, the complementary nucleotides are ad...
Q: What were some of the most interesting learnings in biochemistry?
A: Biochemistry, often known as biological chemistry, is the study of chemical processes inside and rel...
Q: Write the structure of each of the following peptide at pH7 and identify how many peptide bond in ea...
A:
Q: Determine the statement that is not TRUE about Dietary Reference Intake recommendations. DRI recomme...
A: The statement which is not true is:
Q: Explain why amino acid can increase blood glucose.
A: Protein breakdown - It occurs in stomach of humans. Once a protein source reaches your stomach, hydr...
Q: Refer to the following diagram: 100 ul 100 μL 100μι 10 μι 10 μι 100 μι 100 μL Volume to transfer 900...
A: Colony forming unit is used to determine the concentration of the microorganism in the test sample....
Q: During vigorous exercise, a buffer system consisting of lactic acid and sodium lactate is produced i...
A: Given Values: pKa of lactic acid = 4.76 The concentration of lactic acid in muscle cells = 1.5 μM Th...
Q: 4. What would be the most possible interaction between the side chains (R-group) of isoleucine and p...
A: The peptide bond is formed between the alpha amino and the alpha carboxyl groups of amino acids and ...
Q: H. Draw a plot showing reaction velocity as a function of substrate concentration for Ks = 50 µM, Vm...
A: As here given in the question, Vmax= 5uM S-1 Ks=50uM and Ki = 250uM both in presence and absence of ...
Q: Please paraphrase how both experiments (from fig.2 and 3) are asking differing questions
A: Hi, thanks a lot for asking this technical experimental question. As You have asked to paraphrase th...
Q: A solution was subjected to series of test to identify what carbohydrate/s is/are present. Results w...
A: A positive (violet ring between layers) Molich test indicate the sample contains carbohydrates A pos...
Q: Compute for the dilution factor of the WBC pipet if blood is sucked up to 0.2 mark and the diluting ...
A: WBC pipette has markings upto 1 where blood can be sucked and 11 is total capacity of pipette for di...
Q: 5. A 0.01 M solution of HCI will not hurt your skin, yet a 0.1 M solution of HCI will leave a painfu...
A: Hydrochloric acid is a colorless and odorless solution of hydrogen chloride and water with a chemica...
Q: Density lipoprotein,reduces the risk of atherosclerosis by clearing out fatty plaques in the blood v...
A: There is unequivocal evidence of a negative relationship between plasma high-density lipoprotein (HD...
Q: What type of lipid I shown in the picture believe?
A: Lipids are non-polar biomolecules. Lipids are classified as simple lipids, compound lipids, and deri...
Q: Give an example and explain why and how changing just one amino acid in a protein's sequence can aff...
A: Proteins (polymers) are macromolecules made up of subunits of the amino acid (the monomers ). These ...
Q: 6. All 20 amino acids have A. one chiral carbon B. one n-carbon C. one carboxyl group D. ionizable R...
A: Proteins are made up of amino acids. Amino acids are linked by peptide bonds, this simple structure ...
Q: List 2 proteins that facilitate protein folding and briefly describe the role each play in the lifet...
A:
Q: Each of the cytochromes in the mitochondrion contains prosthetic groups. What is a prosthetic group?...
A: Conjugated proteins have prosthetic group with apoprotein. Apoprotein - The protein part of the conj...
Q: Applying: Which of the following commonly acts as the precursor for the synthesis of vitamin C (asco...
A: Carbohydrates are compounds formed of monosaccharides as building blocks, which range from simple su...
Q: 1- Please write any MRNA sequence that produces protein sequences of 'INFRMATICS'. By using your ami...
A: Hi! Since you have posted multiple questions and have not mentioned which to answer, we will answer ...
Q: (A) Do electron transport chains create a gradient so that ATP synthase can generate ATP molecules? ...
A: ATP synthase is a mitochondrial enzyme machinery which is located in the inner membrane of mitochond...
Q: 1.) What nitrogenous base would not be expected in structure C? 2.) What nitrogenous base would not ...
A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which o...
Q: Assuming that G6P is labeled at its C1 position, determine where the label will appear in the formed...
A: Pentose phosphate pathway or hexose monophosphate shunt is an alternative pathway to glucose oxidati...
Q: The discussion this week explores the reasons why carbon dioxide changes the affinity of oxygen to h...
A: Hemoglobin is a respiratory pigment present in red blood cells. It is a conjugated protein and gives...
Q: Explain why those biological reactions that have their equilibria shifted towards the products have ...
A: Thermodynamically favorable reactions have a negative value of the change in Gibb's Free Energy. In...
Q: The following compound was obtained from cells, after supplying them with 1"C-labeled pyruvate. The ...
A: Pyruvate is a three-carbon compound that enters the TCA cycle through the pyruvate dehydrogenase rea...
Q: Evaluating: If a DNA strand reads AATGCTCCAGT, what would the complementary strand read: * O GGCAATC...
A:
Q: . The buffer that is adjusted to control acid-base balanceis ________.a. plasma proteinb. hemoglobin...
A: Carbon dioxide is produced in huge quantities in tissues with a high metabolic rate. Carbon dioxide ...
Q: Crystal: Calcium oxalate or calcium phosphate in kidney stones If it is something harmful, what can...
A: A kidney stone is a solid, marble-like piece of material that forms in one or both kidneys. It forms...
Q: What is the Amino Acid Sequence of 3'-AUG GUA CUC UUA ACA CCA GUG GAA AAA-5'
A: Proteins are polymers made of amino acids. The cellular process by which proteins are synthesized by...
Q: Describe the way a polypeptide is synthesized during translation.
A: Polypeptide refers to sequence of amino acids bonded to each other via peptide bonds. The synthesis ...
Q: how would you identify whether or not complete resolution of the three amino acids has been achieved
A: An amino group and an acid group-containing organic molecules are called Amino acids. when ...
Q: Would any of the above give a positive Lieberman-Burchard test? Explain your answer.
A: The endocrine system is a vital biological system that produces various hormones. These hormones ser...
Q: Why did the soap have to “cure” in the molds?
A: Fats and oils are triglycerides having a glycerol backbone with all the hydroxyl groups esterified w...
Q: lanes for the blue and brown allele samples? (circle one) Brown sample: 0 1 Blue sample: 0 1 2 more ...
A: Agarose Gel electrophoresis is the molecular biology technique which helps in separation of gene se...
Q: topic: Bradford Assay There are numerous methods of protein determination in use, but this module fo...
A: The Bradford assay is a dye-binding assay that is based on the changes in color of the Coomassie dye...
Q: After sufficiently long and intense exercise, EPOC can be observed for more than a day True False
A: Excessive Postexercise Oxygen consumption is the phenomenon by which our body continues to burn calo...
Q: Applying: Partial fermentation of cane sugar by Leuconostoc mesenteroides produce: glucose and gluco...
A: Lectins - Lectins are carbohydrate-binding proteins that are highly specific for sugar groups that a...
Q: 2. The diagram below shows the structure of a sugar. ÇH2OH C=0 но H но H- ČH2OH Is the sugar an aldo...
A:
Q: Which of the following will occur if there is an increase in enzyme or substrate concentration? in...
A: Enzymes increase the rate of biochemical reactions by decreasing the activation energy. Activation e...
Q: Why is BSA used as a standard for protein content determination? Can other proteins be used instead?
A: Bovine Serum Albumin is a protein which is derived from cows. It consists of 583 amino acids, bound ...
Q: Define about cis-regulatory sequences on the mRNA ?
A: mRNA - It has the tripartite structure with a 5' untranslated region( UTR), and coding region follow...
Q: 2. The standard state in biochemistry is slightly different than in Physical Chemistry. In biochemis...
A: "Since you have posted a question with multiple sub-parts, we will solve first three subparts for yo...
Q: What is the likely mechanism of spread of NDM-1?
A: NDM-1 is the protein product of a gene that some bacteria produce. A bacterial strain containing NDM...
Q: Discuss and describe the five major groupings of amino acids.
A: Amino acids are compounds containing carbon, hydrogen, oxygen, and nitrogen. They are the building b...
Q: H. Draw a plot showing reaction velocity as a function of substrate concentration for K, = 50 µM, %3...
A: Having a high Vmax means that all of the binding sites are being used again and again. The dissocia...
Q: 1. What trend did you observe for the rate of peroxidase compared to the variable tested?
A: The enzyme peroxidase is responsible for catalyzing the reaction which converts H2O2 to H2O. Here th...
Based on the structure of CBB G-250, explain why it can bind to proteins having basic and hydrophobic amino acid residues.
Step by step
Solved in 3 steps
- Shown to the right is a cartoon image of ETC Complex I from Y. lipolytica. A group of authors studied this wild type Complex I and also a version where the arginine at position 121 in the chain 3 subunit was mutated to a methionine (R121M). Part of chain 3 subunit contains the peptide sequence SMITH a. Draw the titration curve for the SMITH peptide. Mark all pKa’s and the pI on your curve. For the SMITH peptide at physiological pH, explain what types of interactions you believe the side chains are or are not able to participate in.Certain naphthalene derivatives, such as the dansyl group, exhibit a weak yellow fluorescence when they are in a highly polar environment (such as water) and an intense blue fluorescence when they are in a markedly nonpolar environment (such as hexane). The binding of ´ -dansyl-lysine to specific antibody is accompanied by a marked increase in its fluorescence intensity and a shift in color from yellow to blue. What does this finding reveal about the hapten–antibody complex?A tetrapeptide, glutamate-glycine-alanine-lysine, is prepared at at concentration of 1 mM (0.001 M) and is measured in the standard setup (pathlength of 1 cm). What is the approximate absorbance of this peptide at 280 nm? Hint: if the peptide contained a single tryptophan, the answer would be about 10. 10 280 1 0
- Explain why cleavage of the bond between arginine 15 and isoleucine 16 of chymotrypsinogen activates the zymogen.TLCK and TPCK both use Histadine residues in the active site as inhibitors of serine proteases. Why is TLCK specific for trypsin and TPCK is specific for chymotrypsin?The affinity of hemagglutinin for sialic acid is quite low, with a Kd of approximately 5 mM. How does efficient attachment occur with such a low-affinity interaction?
- Crystal structures exist for three neurokinin-1 (NK1) ligand complexes with the following pdb codes (6hll, 6hlo,6hlp). State which is the highest quality crystal structure indicating the criteria you used to evaluate this.Classify the symmetrical arrangement ofthe subunits (e.g., C2 , C3 , D 2 , helical, icosahedral). 1. Proteobacterial nitrite sensor (3PNS):2. Pseudomonad oligosaccharide receptor (7POR):3. Adenoviral structural protein (8ASP)When starved, bacteria produce the intracellular signaling molecule ppGpp (guanosine-3′,5′-bisdiphosphate). Draw its structure.
- Saccharide M, a sugar made of repeating units of maltose and cellobiose, is a component of glycoproteins 1 and 2, which are receptors found in the membrane of Shrek's cells. For glycoprotein 1, saccharide M is attached by an a 1® 2 linkages to 3 sialic acid residues, then O-linked to the protein, while glycoprotein 2 has saccharide M attached by a b 1®3 bond also to 3 sialic acid residues, then it is also O-linked to the protein. Glycoprotein 1 is a transporter protein for glucose, while glycoprotein 2 is the Na*-K*receptor/ transporter. The glycoproteins show 99% sequence homology of its amino acid sequence. a. Explain briefly why there is a difference in the functions of glycoprotein 1 and 2 b. Glycoprotein 1 is a receptor for glucose. Using Benedict's reagent, tests on the glycoprotein gave NEGATIVE results. Give 2 amino acids that can O-link with Saccharide M and show the portion of both protein and saccharide where a bond will be formed.The ACE2 receptor protein in humans has been getting a lot of press these days, as it apparently serves as a "receptor" for the SARS-CoV-2 coronavirus in addition to its normal functions. This protein has an isoelectric pH of 5.36. Suppose you want to purify this protein to study its properties, with the goal of developing a drug that would block it from binding the virus. Once you have purified the cell surface proteins from your cultured human lung cells away from all the other types of molecules in the cell, and all the cytoplasmic proteins, you might decide to use ion exchange chromatography to separate the ACE2 proteins from other proteins in your sample. If you plan to load your protein mixture onto your ion exchange column in a buffer at pH 7.4 (approximately physiological pH) and you want the ACE2 proteins to bind to the column, should you use a resin with a positive charge or a negative charge? Briefly explain why you chose the resin you selected.Trypsin cleaves proteins on the carboxyl side of lysine. Trypsin inhibitor has a lysine residue, and binds to trypsin, yet it is not a substrate. Explain.