Based on the N-terminal amino acid sequence, what is the approximate half-life of the protein after methionine is cleaved off
Q: What is the resulting polypeptide: _______________________________________________________? What…
A: Mutation in a gene sequence causes encoding of a mutated protein. This mutated protein will have…
Q: Why are misfolded proteins a potential problem for the eukaryotic cell, and how do cells combat the…
A: Eukaryotic cells can be defined as the cells that are complex in terms of structure and function as…
Q: A glycine residue is in position 210 of the tryptophan synthetase enzyme of wild-type E. coli. If…
A: Single base substitutions in which change in only one base pair occurs.
Q: If a protein is created via the translation of 705 nucleotides, including a start and a stop codon,…
A: Central dogma is the process in which RNA is produced from the DNA (transcription) and amino acid…
Q: What is single-cell protein? Explain the reasons for the removal or reduction of nucleic acid in…
A: Protein is a substance formed of amino acids joined by peptide bonds. Nucleic acid - are compounds…
Q: What are the possible codons that a tRNA molecule could interact with if it is carrying each of the…
A: A codon is a triplet nucleotide of DNA or RNA corresponding during protein synthesis to a particular…
Q: Are tRNAfmet and tRNAmet made from the same aminoacyl tRNA synthetase?
A: Amino acyl tRNA synthase pairs tRNA with amino acid that their anti codon codes for. Amino acyl tRNA…
Q: Which of the sequences below do you think would most likely be found in an intrinsically disordered…
A: Intrinsically disordered proteins (IDPs) are those that do not have a proper three-dimensional…
Q: Describe the common strategy (steps) for protein sequencing, starting with a biological sample…
A: Proteins are one of the 4 major biomacromolecules. Proteins are the most abundant of the 4…
Q: The protein encoded by the cystic fibrosis gene is 1480amino acids long, yet the gene spans 250 kb.…
A: Because the gene contains introns (non-coding regions within the gene) that are removed during the…
Q: If a tRNA has an anticodon sequence 5'-CAU-3', What would be an amino acid carried by that tRNA?
A: Codon is defined as the the group of three nucleotides that encode an amino acid.
Q: Lactose permease, a protein of E. coli, is composed of a single polypeptide that is 417 amino acids…
A: Lactose permease Its belong to transmembrane protein family. The transport mechanism of lactose…
Q: What does the third position imply when it comes to tRNA wobble effect and what the wobble effect…
A: Wobble base pairing : It is a pairing between two nucleotides in RNA molecules . The four main…
Q: How many activation cycles, Initiation cycles, Elongation cycles and termination cycles are needed…
A: Protein synthesis occurs in four main steps such as activation or charging of tRNA, initiation of…
Q: A normal hemoglobin protein has a glutamic acid at position 6; in sickle-cell hemoglobin, this…
A: In the normal hemoglobin molecule protein, has a glutamic acid (an amino acid) at the sixth position…
Q: The following polynucleotide was synthesized and used as a template for peptide synthesis in a…
A: The DNA template is used to form an mRNA polynucleotide by the process of transcription. The mRNA…
Q: A normal polypeptide and a mutant of the polypeptide were hydrolyzed by an endopeptidase under the…
A: Chromatography is a useful technique to study the separation of amino acids. Paper chromatography is…
Q: Describe the process where a peptide chain is formen from a code embedded in a nucleic acid…
A: Central Dogma of molecular Biology explain how a DNA replication result into DNA, DNA is changed…
Q: What are the sequences of all the possible tripeptides that contain the amino acids, serine, leucine…
A: The polypeptide is made up of amino acid residues, which are joined by the peptide bond.
Q: When glycoproteins are synthesized in the cell, at what stage of the polymerization of the protein…
A: Proteins with covalently linked sugar residues are known as glycoproteins. Sugars' hydrophilic and…
Q: Figure shows a part of the sequence alignment of human and whale myoglobin proteins. Please explain…
A: The condition represented in the sequence alignment is amino acid replacement. In amino-acid…
Q: (a) How many activation cycles are needed for a protein with 150 amino acids? (b) How many…
A: The process of translation or protein synthesis occurs in the cytoplasm of a cell in both…
Q: How many amino acids long is a zinc finger domain? What four conserved residues are contained in the…
A: Zinc finger are structural protein motif that has Zn ion in a coordinated bond to form a stable…
Q: Which of the following are elongation factors involved in the attachment of new aminoacyl-tRNAs?…
A: During translation protein synthesis occurs from RNA. The sequence of nucleotides in RNA act as code…
Q: what is the n-terminal AA residue? use one-letter code for amino acid
A: The N-terminal amino acid residue is the amino acid that is present in the N-terminal. For protein…
Q: What method could be used to make an immobilised library of 6 different peptides, all differing by…
A: INTRODUCTION Peptide-based molecular probes identified by bacteriophage (phage) display technology…
Q: A. What amino acid sequence is encoded by the codon sequence AUAAUGGUAACGGUU? B. Suppose the codon…
A: DNA is the carrier of genetic information in almost all organisms except certain RNA viruses such as…
Q: What is the significance of the positioning of the amino acid side chains and alpha carbons as you…
A: Proteins are polymers of amino acids linked by peptide bond/amide bond. Peptide bond is a covalent,…
Q: The arrangement of amino acids in the primary sequence affects the secondary, tertiary, flexibility,…
A: The sequence of amino acids in a protein (the primary structure) will determine where alpha helices…
Q: Given that a faulty ribosomal protein is the culprit and causes DBA, discuss the possible role of…
A: The bone marrow does not create sufficient red blood cells to carry oxygen across the body in cases…
Q: what is the anticodon sequence that would build this protein?
A: The process of formation of mRNA sequence from a DNA transcript is known as translation. In mRNA,…
Q: How many cases are there in which it would be possible to identify the first two nucleotides of a…
A: Codon is a three nucuclotide sequence on mRNA that determines the amino acid sequence in newly…
Q: How can widely separated parts of a protein interact with the spike protein
A: Spike proteins are glycoproteins that are present on the surfaces of the enveloped viruses of many…
Q: . The following synthetic polynucleotide is synthesized and used as a template for peptide synthesis…
A: Polypeptide is made up of chain of amino acids linked together. Polypeptides are connected together…
Q: How does aminoacyl-tRNA synthetase enzyme select and determine the right tRNA for activation?
A: The aminoacyl-tRNA synthetases identify the proper tRNAs based on their entire configuration rather…
Q: If a mutation changes base triplet 1 from ATG to ATA, why will this not change the protein formed
A: If a mutation changes base triplet 1 from ATG to ATA, why will this not change the protein formed?…
Q: There are different tRNAs for each amino acid. What is one major way to differentiate among the…
A: During the process of translation, the proteins are synthesized from the translational complex,…
Q: How would the the triple mutations K557R and D591V and K617A alter the overall protein?
A: Here, 1.) K means lysine(positively charged amino acid) 2.) R means Arginine(positively charged…
Q: The infectious prion protein which are believed to cause a neurodegenerative disease by acting as a…
A: neurodegenerative diseases which are due to the infected prions can occur both in animals and in…
Q: What would be the minimum length(approximate number of bases) of an mRNAthat coded for a protein 300…
A: To determine: To determine the required minimum length of an mRNA that is coded for a protein of 300…
Q: Describe why this position in your protein is important and outline the effects the mutation will…
A: Any change in DNA is referred to as a mutation. Any heritable and genetic modification in the genome…
Q: What are the amino acids carried by tRNAs if their anticodons are either 5'-CAU-3' and 3'-GUC-5'?
A: m RNA carries the codon and t RNA carries the anticodon. t RNA brings amino acid according to the…
Q: An alanine residue exists at position 180 of a certain plant protein. If the codon specifying…
A: Substitution Replacement of one Nitrogenous base by another Nitrogenous base is called as…
Q: Is there a difference in the effects of different salt solutions on protein denaturation? Why?
A: Yes, there is a difference in the effects of different salt solutions on protein denaturation.…
Q: What is a motif? Why is it useful for computer programs to identifyfunctional motifs within amino…
A: Bioinformatics has permitted molecular biologists to store and analyze a large amount of data…
Q: If the gene undergoing protein synthesis consists of 24 bases, how many codons does that result in?…
A: In genetic code a unit known as codon, which codes for amino acid. For example the sequence AUG is a…
Q: The human genome contains thousands of sequences known as small open reading frames, some of which…
A: Deoxyribonucleic acid (DNA) is a genetic material and it carries genetic information from one cell…
Q: What is the resulting polypeptide: _______________________________________________________? What…
A: Biomolecules are organic molecules present in living organisms. They include carbohydrates,…
Based on the N-terminal amino acid sequence, what is the approximate half-life of the protein after methionine is cleaved off?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- cggcaggagc cccccgacct cccaggcgga ccgccctccc tccccgcgcg cgggttccgg gcccggcgag agggcgcgag cacagccgag gccatggagg tgacggcgga ccagccgcgctgggtgagcc accaccaccc cgccgtgctc aacgggcagc acccggacac gggcctcagcc actcctacat ggacgcggcg cagtacccgc tgccggagga ggtggatgtg ctttttaaca tcgacggtca aggcaaccac gtcccgccct actacggaaa ctcggtcagg Provide the primers for the nucleotide sequence above (GATA3)gene5'-AATGCCTCAGCCGATCTGCCTCGAGTCAATCGA TGCTGGTAACTTGGGGTATAAAGCTTACCCATGGTATCGTAG TTAGATTGATTGTTAGGTTCTTAGGTTTAGGTTCTGGTATT GGTTTAGGGTCTTTGATGCTATTAATTCTTTGGTTTTGATTT GGTCTTTATATGGTTTATGTTTTAAGCCGGGTTTTGTCTGG - GATGGTTCGTCTGATGTGCGCGTAGCGTGCGGCG - - - 3' What are the sequences of the investigator's forward and reverse primer? Enter them 5' to 3'. forward primer: reverse primer:+ www-awu.aleks.com/alekscgi/x/Isl.exe/10_u-lgNslkr7j8P3JH-IQUHIQg6bJxmeSyVPHOEB1plef9xyC5Ca9QlbCmqT-TP-BcuGkqzvM9h_eXu2dblOXnFt00g1aPGkUe7G4... с O Biological Macromolecules Classifying mutations A certain section of the coding (sense) strand of some DNA looks like this: ATGAGTATCTAA It's known that a very small gene is contained in this section. In the table below, write the base sequence for two point mutations of this DNA, one silent, and one not. Silent mutation: Non-silent mutation: Explanation Check ☐ MacBook Air 0/5 Julianna X $ 00. 园 2024 McGraw Hill LLC. All Rights Reserved. Terms of Use | Privacy Center | Accessibility
- C UUU UUC Phe UUA UCU) UCC UCA UAU UAC Tyr UGU UGC Cys Ser UAA Stop UGA Stop A UAG Stop UGG Trp UUG FLeu UCG CUU CUC CCU] СС CAU1 CAC САC His САА Gln CGU CGC Arg Leu Pro CUA CUG ССА CGA CUG J CCGJ Which of the following tRNA anticodons could theoretically base pair effectively with the codons 5'-AAU-3' and 5'-AAC-3' through third-base CAG, CGG AGU AAUASN AGC AUU AUC File A AUA ACU АСС АСА AAC FAsn AAA Ser C Thr AGA Arg A AUG Met ACG AAG Lys AGG GCU] GCC GCA GCG GGU GGC Gly GGA GAU1 GUU GUC GUA GUG Asp GACJ GAA C Val Ala A GAG Glu GGG wobble? 5'-GGG-3' OI. 5'-AUG-3' II. 5'-GUU-3' I. 5'-CAT-3' IV. 5'-AAA-3 V. Third letter UCAG UUAG A, First letterC A UUU U UUC Phe UCU UCC UCA UAU UAC UAA UAG UGU Cys U UGC Stop UGA Stop Stop UGG Trp G Tyr Ser UUA UUG Leu UCG CUU C CUC CUA CCU Leu ccc CCA CCG CAU CAC CAA His CGU CGC CGA CGG Pro Arg 1st Gin CUG CAG 3rd letter AUU A AUC AUA AUG ACU AAU AAC Ser u letter C Asn AGU AGC AGA AGG lle ACC Thr ACA AAA AAG Lys Arg Met ACG GUU G GUC GUA GUG GCU GCC GCA GCG GAU GAC GAA GAG Asp GGU GGC GGA GGG Val Ala Gly Glu Not all of the following ingredients are necessary for an in vitro translation reaction. Which one would you leave out? A ssDNA template O Cell sap containing ribosomes poly(U) O 14C-PheFirst base U A G U UUU UUC UUA UUG CUU CUC CUA CUG Phe GUU GUC GUA GUG Leu Leu AUU AUC lle AUA AUG Met or start Val Second base C A UCU UCC UCA UCG CCU CCC CCA CCG ACU ACC ACA ACG GCU GCC GCA GCG Ser Pro Thr Ala UAU UGU Tyr Cys UAC UGC UAA Stop UGA Stop A UAG Stop UGG Trp CAU CAC CAA CAG AAU AAC AAA AAG GAU GAC GAA GAG His Gin Asn Lys Asp G Glu CGU CGC CGA CGG AGU AGC AGA AGG GGU GGC GGA GGG Arg Ser Arg Gly UCAG UCAG ט כ с AG А U ט כ C G Third base A mutation in the DNA that codes for hemoglobin changes the sequence CTC to CAC. This will cause a change in the mRNA from (original) ? to (mutated) __?____, resulting in the addition of the amino acid, (mutated) ____?___, instead of (original) ____?_
- From the above sequence I have copied the first 500bp of the sequence into Word. ATGGTAAAGGCCGTCGTCGGATTCGGCGCTGCATGCGGAATCT CTGCTATCGTTGCTTGCCTTTGGGCTGCACTTGTCATCACAAA TGACATCAATGACATGTATGATGATGTGATGGGAGAGCTCGGA GGATTCAGAGATATCTCTGATGACACTTGGGGAACCCTTCTCG ACGTTCGTCACGGAGCCGGAGAGTCTGCTGAGCAATACGTTCG TGGAATCTTCGGACGTCACAAGCGTTCCAACAGCCAATGCTCT TGCGGACTTCCATCTCAAGGATGCCCAGCCGGAGCTCCAGGAA ACCCAGGAGCCCCAGGAGAGCCAGGAGGCACTGGACCAGACGG AAAGAACGGACCAACTGGACTTCCAGGACTTAACATTCCAATT CCAAATGACTTCCCTAAGGAGTGCATCAAGTGCCCAGCTGGAC CACCAGGACAAGATGGACTTCCAGGACAAGAAGGATTCCAAGG ACTTCCAGGAGACGCTGGAAAGCGTGG 1) You now need to design primers to amplify this 500bp region of the DNA. Write out the primer sequences in the correct orientation. 2) Decide on two restriction sites that you can use to clone this into pL4440's MCS. Identify their sequence. Tip: The plasmid map is in Figure 3, details of restriction site sequences can found https://enzymefinder.neb.com/#!#nebheader atFrom the above sequence I have copied the first 500bp of the sequence into Word. ATGGTAAAGGCCGTCGTCGGATTCGGCGCTGCATGCGGAATCT CTGCTATCGTTGCTTGCCTTTGGGCTGCACTTGTCATCACAAA TGACATCAATGACATGTATGATGATGTGATGGGAGAGCTCGGA GGATTCAGAGATATCTCTGATGACACTTGGGGAACCCTTCTCG ACGTTCGTCACGGAGCCGGAGAGTCTGCTGAGCAATACGTTCG TGGAATCTTCGGACGTCACAAGCGTTCCAACAGCCAATGCTCT TGCGGACTTCCATCTCAAGGATGCCCAGCCGGAGCTCCAGGAA ACCCAGGAGCCCCAGGAGAGCCAGGAGGCACTGGACCAGACGG AAAGAACGGACCAACTGGACTTCCAGGACTTAACATTCCAATT CCAAATGACTTCCCTAAGGAGTGCATCAAGTGCCCAGCTGGAC CACCAGGACAAGATGGACTTCCAGGACAAGAAGGATTCCAAGG ACTTCCAGGAGACGCTGGAAAGCGTGG 1) You now need to design primers to amplify this 500bp region of the DNA. Write out the primer sequences in the correct orientation. 2) Decide on two restriction sites that you can use to clone this into pL4440's MCS. Identify their sequence. Tip: The plasmid map is in Figure 3, details of restriction found at site sequences can be https://enzymefinder.neb.com/#!#nebheader 3) Add the restriction site…From the above sequence I have copied the first 500bp of the sequence into Word. ATGGTAAAGGCCGTCGTCGGATTCGGCGCTGCATGCGGAATCT CTGCTATCGTTGCTTGCCTTTGGGCTGCACTTGTCATCACAAA TGACATCAATGACATGTATGATGATGTGATGGGAGAGCTCGGA GGATTCAGAGATATCTCTGATGACACTTGGGGAACCCTTCTCG ACGTTCGTCACGGAGCCGGAGAGTCTGCTGAGCAATACGTTCG TGGAATCTTCGGACGTCACAAGCGTTCCAACAGCCAATGCTCT TGCGGACTTCCATCTCAAGGATGCCCAGCCGGAGCTCCAGGAA ACCCAGGAGCCCCAGGAGAGCCAGGAGGCACTGGACCAGACGG AAAGAACGGACCAACTGGACTTCCAGGACTTAACATTCCAATT CCAAATGACTTCCCTAAGGAGTGCATCAAGTGCCCAGCTGGAC CACCAGGACAAGATGGACTTCCAGGACAAGAAGGATTCCAAGG ACTTCCAGGAGACGCTGGAAAGCGTGG 1) You now need to design primers to amplify this 500bp region of the DNA. Write out the primer sequences in the correct orientation.
- Sequence A uuucccucuuagaauuaauucguaauauuuaucau uuaaauuuagcucccuccccccauuaauaaauaauu cuaucccaaaaucuacacaauguucuguguacacuu cuuauguuuuuuacuucugauaaacguuuuugaaaa aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa Sequence B uuucccucuuagaaugaaucucguaaugaaaaguc gaaaaaaauugugcucccuccccccauuaauaaau aauucuaucccaaaaucuacaca augu ucuguguacacuucuuauguuuuuuacuucugauaa acguuuuugaaaaaaaaaaaaaaaaaaaaaaaaaaa aaaaaaaaaa Q1a) Underline the Poly A signal in both sequences. Q1b) What is the sequence of the translational stop codon? Q1c) Which of these two mRNA isoforms is likely to be more stable? Q1d) Justify your answer to part Q1c).Second letter UUU UUC UUA UUG. UCU UCC UCA UCG UAU Tyr UAC. Ser UAA Skp UGIA Stop UGU UGC. Phe Cys Leu UAG Sop UGG Trp CGU CGC Arg CGA CCU CAU CUU CUC CUA CUG His CAC CAA CAG Gin CCC Pro CCA Leu CCG CGG ACU AAUA AGU ]Ser AUU Asn AAC AGC AGA LArg AGG. AUC Be ACC ACA Thr AAA AUA AUG Met ACG MGys GUU GUC Val GUA GCU GCC GCA GCG Ala GAA Glu GAG GAU GAC Asp GGU GGC Gly GGA GGG GUG You come across four polynucleotide strands. The first is an original RNA strand that codes for a protein; the others are similar to the original RNA strand, except that each displays a point mutation. The point mutations are shown in red. 5' - AUG-UGC-AUU-CCU-GCA-UAA - 3' 5' - AUG-UGA-AUU-CCU-GCA-UAA - 3' 5' - AUG-UGC-AUU-CCC-GCA-UAA - 3' 5' - AUG-UGC-GUU-CCU-GCA-UAA - 3' Original RNA: Mutant RNA 1: Mutant RNA 2: Mutant RNA 3: 1. Based on how they will affect the protein product, identify the type of point mutation (missense, nonsense, silent, or frameshift) present in the Mutants 1, 2, and 3. 2. State which of…Second letter C UUU UGU UGC. UUC Phe UUA UUG UCU UCC UCA UCGJ UAU1 UAC. Ser UAA Stop UGA Stop A Tyr Leu UAG Stop UGG Trp CAUTHIS CACS CAA GIn CAG CGU CGC Arg CCU CUU CỤC CỦA CUG J CC Leu Pro ССА CGA CCGJ CGGJ AGU Ser ACU АСС ACA AUU AAU Asn AAC AAA AUC le AGA TArg Thr AUA AUG Met ACG AAGLYS AGG GAU GUU) GUC GUA GUG GCU) GCC GCA GACASP GAA AGlu GAGS GGU GGC Gly Val Ala GGA GGG GCGJ Given the following mRNA sequence: 5' GCCCAUGUGGCGCGAGUGAUUUAA 3' Third letter UCAC UCAG UCAG UCAG 등 | First letter