How would the the triple mutations K557R and D591V and K617A alter the overall protein?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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The authors state: "Properties of the single mutants K557R, D591V and K617A, a double mutant,
D591V/K617A, and a triple mutant, K557R/D591V/K617A, were evaluated to find an allosteric
binding site for citrate."
The numbers between the letters represent the residue number altered. The letter in front was the
wild type and the letter afterwards is the mutant.
How would the the triple mutations K557R and D591V and K617A alter the overall protein?
O lower the # of charges, increase the pl
O increase the # of charges, increase the pl
O lower the # of charges, lower the pl
O lower the # of charges, increase the pl
O no change in the # of charges, increase the pl
O no change in the # of charges, lower the pl
Transcribed Image Text:The authors state: "Properties of the single mutants K557R, D591V and K617A, a double mutant, D591V/K617A, and a triple mutant, K557R/D591V/K617A, were evaluated to find an allosteric binding site for citrate." The numbers between the letters represent the residue number altered. The letter in front was the wild type and the letter afterwards is the mutant. How would the the triple mutations K557R and D591V and K617A alter the overall protein? O lower the # of charges, increase the pl O increase the # of charges, increase the pl O lower the # of charges, lower the pl O lower the # of charges, increase the pl O no change in the # of charges, increase the pl O no change in the # of charges, lower the pl
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