Another restriction enzyme is called HaellI. It cuts DNA at the following base sequence: CCGG GGCC It cuts between the C and the G as follows: ccGG GGCC 1. Show the DNA fragments that would result if Haelll was used to cut the DNA fragment shown in diagram 1. TACCGGGAATTCATCCGGTGAATTCTAGCGTAC ATGGCCC TTAAGTAGGCCACTTAAGATCGCATG
Q: A circular DNA of 40 kb (40,000 bases) length is cut with a restriction enzyme whose precise…
A: The probability of a restriction enzyme to be found in a DNA is 4n, where n is the number of bases…
Q: A piece of human DNA is treated with two common restriction enzymes. The DNA piece in question is 50…
A: Restriction enzymes are a specific group of enzymes that can recognize a specific short sequence of…
Q: Given the DNA strand with sequence 5' AUGGAGGAUGGCCAGUCAAUUUGA 3' match the various mutations to the…
A: Codon is a sequence of three nucleotides that corresponds to a specific amino acid. Codons encode…
Q: The restriction enzyme BamHI recognizes the sequence GGATCC and cuts the DNA between the two G’s.…
A: Ans: Restriction enzymes: The enzymes which cuts the DNA at specific site and used in cloning is…
Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…
A: An enzyme that recognizes a specific sequence on the DNA strand and cuts the DNA into fragments is…
Q: EcoR1 is a restriction enzyme that cuts DNA at the following base sequence: 5' GAATTC 3' Which…
A: The restriction enzyme cuts the DNA at the specific recognition sequence.
Q: Most prokaryotic organisms produce one or more enzymes to defend themselves against the infection by…
A: Bacteria constitute a wide domain of prokaryotic microbes with majorly varying lengths and a number…
Q: A new restriction enzyme is discovered. It recognizes a 6 base pair pallindromic sequence. The first…
A: BASIC INFORMATION RESTRICTION ENZYME they are also known as molecular scissors it was in the year…
Q: A researcher wants to cut this piece of linear DNA. 5 AGCTAGTCCGGATCCGAGGGCCCTTCTCATGAGATCCGGATGACC…
A: The discovery of restriction enzymes in 1968 by Swiss scientist "Werner Arber" ushered in the era of…
Q: Which of the restriction enzymes listed in the table below produces blunt-end fragments? Enzyme…
A: The blunt end are produced by the restriction enzymes when the end of a DNA fragment after breaking…
Q: The restriction enzyme Smal recognizes the following sequence and cuts it once between the G and C:…
A: Answer. A restriction endonuclease is a bacterial enzyme that cuts double-stranded DNA by cleaving…
Q: A linear piece of DNA is cut by two restriction enzymes. One restriction enzyme will cut the target…
A: Restriction enzyme : These are also known as restriction endonucleases , a protein produced by the…
Q: h of the following is NOT true of cutting DNA in biotechnology? A molecular biologist can cut DNA…
A: Biotechnology is a technique in which DNA is manipulated either by cutting the the and required gene…
Q: What will be the newly synthesized DNA from the template given? DNA Template 3 - CGGATGELLGTATAL -5…
A: DNA replication :- It is the formation of a new DNA from a pre existing DNA. The DNA so formed is…
Q: The partial sequence of one strand of a double-stranded DNA molecule is…
A: Restriction endonucleases are the enzymes used in genetic engineering or DNA cloning in which…
Q: A researcher wants to cut this piece of linear DNA. 5'…
A: The restriction enzymes are the specific enzymes that act on the DNA molecule and perform its…
Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…
A: The corner stone of genetic engineering or recombinant DNA technology is a special class of enzymes…
Q: If you have got the following DNA template molecules, which one of them will require more energy to…
A: Melting temperature is the temperature at which a double-stranded molecule of DNA is broken down…
Q: In the process of genetic engineering, recombinant DNA is produced by combining genetic material…
A: Recombinant DNA technology consists of techniques that are developed for manipulating, maintaining,…
Q: Which two restriction enzymes where used to completely digest the DNA fragment above based on the…
A: Restriction endonucleases are the enzymes which cleave the DNA at specific sites from within and…
Q: The same restriction endonuclease must be used to excise the foreign DNA and bacterial DNA. Select…
A: DNA or deoxyribonucleic acid is a double-stranded molecule, strands of which are coiled around one…
Q: A molecule of double-stranded DNA that is 5 million base pairs long has a base composition that is…
A: If a molecule of double-stranded DNA which is 5 million base pairs long, has a base composition that…
Q: The following double stranded DNA fragment was double digested with BamHI and Kpnl. If you ran the…
A: Restriction enzymes are the enzymes which cut the DNA at specific sequences. The specific sequence…
Q: GAATTC GAATTO CITAAG CITAAG double-stranded DNA DAATT DAATTO CTAA G CTAA GI AAT TO CTTAA GAATTO…
A: The cloning is routinely used in biotechnology laboratories and it is the process by which a foreign…
Q: A purified piece of DNA of 1650bp is cut with Hind III and Pst I separately and then with both…
A: Restriction enzymes are endonucleases derived from bacteria that make a double-stranded cut in the…
Q: Which of the following is most likely to be a restriction enzyme recognition sequence? O AAAAAA O…
A: A palindromic sequence is a sequence of nucleic acids within double helix of DNA and / or RNA that…
Q: What will be the newly synthesized DNA from the template given? DNA Template 3 - CGGATGCCCGTATAC -5…
A: DNA stands for deoxyribonucleic Acid. It is a long polymer of deoxyribonucleotides. It is found in…
Q: Dr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions…
A: Restriction enzymes are the enzymes which cut DNA at particular site.
Q: he sequences below indicated the 6bp recognition site for the restriction enzyme EcoRI. The lines…
A: EcoRI is a restriction endonuclease that is isolated from E. coli and is used in the gene cloning…
Q: Which of the enzymes from the following list wouldyou need to sequence DNA? What is the function…
A: DNA sequencing is a process in which the sequence of the nitrogenous bases is determined in the…
Q: Which of the sequences below would serve as a PCR primer that would bind this DNA strand: 5'-…
A: PCR is polymerase chain reaction. It is a method of DNA amplification developed by Kerry Mullis.…
Q: A molecule of double-stranded DNA that is 5 million base pairs long has a base composition that is…
A: The types of nucleotide bases of the genetic material are the same in all the species present on…
Q: The partial sequence of one strand of a double-stranded DNA molecule is5′ – – –…
A: Restriction enzymes also called molecular scissors are originally a part of a bacterial defence…
Q: Which of the enzymes from the following list wouldyou need to make a recombinant DNA molecule?…
A: The recombinant DNA is where the two or more DNA molecules are integrated into a vector plasmid to…
Q: A DNA synthesizer “machine” is used to create short single stranded DNA of any given sequence. You…
A: A Deoxyribonucleic acid (DNA) synthesizer machine used for generating shorter segments of DNA that…
Q: For the chromatogram below, what is the sequence of the template DNA from base 115 to 125? CTGTGTGAA…
A: DNA is formed of four types of nitrogenous bases, which are adenine, thymine, guanine, and cytocine.…
Q: A 10 kb DNA fragment digested with the restriction endonuclease EcoRI yields fragments of 4 kb and 6…
A: Restriction enzymes are also known as restriction endonucleases. These are the enzymes produced by…
Q: A circular DNA molecule is subjected to complete restriction digestion by (1) BamHI alone, (2)…
A: Restriction enzymes cut DNA at a specific location called as restriction site.
Q: Which restriction endonucleases would cleave a DNA molecule at the given sequences? The…
A: Restriction endonucleases are found in bacteria and they cut double-stranded DNA at the…
Q: #2 EcoRI --- 5’ G ↓AATTC 3’ 5’ ACG ACGTATTAGAATTCTTA TCCGCCGCCGGAATTCT CATCA 3’ 3’ TGC…
A: Restriction enzymes is an enzyme that cleaves DNA into fragments. Recognition sequence is a unique…
Q: Please answer both parts, a. The enzyme that catalyzes the joining of fragments to form recombinant…
A: The recombinant DNA technology is a branch of biology that deals with different techniques that…
Q: Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting…
A: Restriction enzymes are also known as molecular scissors because they cut the DNA at specific, short…
Q: If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on…
A: The restriction enzyme is a protein that identifies a specific nucleotide sequence and cuts the DNA…
Q: A small DNA molecule was cleaved with several different restriction nucle- ases, and the size of…
A: Introduction: DNA is a type of nucleic acid that is present in the nucleus of the cell. It is the…
Q: The restriction enzyme Haelll has the recognition sequence below and cuts between the G and bases.…
A: restriction enzymes are the specific endonucleases that have a specific site within the DNA sequence…
Q: The following DNA fragment shows where a number of restriction endonucleases cut sites occur within…
A:
Q: FIGURE 2 shows the only suitable DNA restriction site in a plasmid DNA vector that can be cleaved.…
A: Restriction enzymes are the most important tool in genetic engineering. These enzymes have the…
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
Resriction Enzyme Worksheet #1
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Problem #2 DNA TACT TAA A AAT G A (TS) DNA (CS) MRNA Amino Acids: Please continue to the next page. Activity 2- Reinforcing Protein Synthesis Use your codon chart to determine the amino acid sequence. Read through the strand and ONLY start on AUG and STOP when it tells you to stop. Follow example below: Example: DNA > AGA CGG TAC CTC CGG TGG GTG CTT GTC TGT ATC CTT CTC AGT ATC MRNA > UCU GCC AUG GAG GC ACC CAC GAA CAG ACA UAG GAA GAG UCA UAG protein > start - glu - ala -thre - hist – asp -glu – threo - stop 1. DNA → CCT CTT TAC ACA CGG AGG GTA CGC TAT TCT ATG ATT ACA CGG TTG CGA TCCATA ATC MRNA protein > 2 DNA → AGA ACA TAA TAC CTC TTA ACA CTC TAA AGA CCA GCA CTC CGA TGA ACT GGA GCA mRNA > pratein > 3. DNA > TAC CTT GGG GAA TAT ACA CGC TGG CTT CGA TGA ATC CGT ACG GTA CTCGCC ATC MRNA > protein → 4. Fill in the diagram below. DNA MRNA ERNA Amino Acids Please continue to the next page. Universal Genete Code Chart Mensenger RNA Codans and the Amina Asd for Which They Code SECONDBASE…BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165Task 1. Your graduate advisor asks you to amplify the following sequence of DNA by PCR: 5’-ATACGCATTCGGACCAGGTCCTAA-3’ 3’-TATGCGTAAGCCTGGTCCAGGATT-5’ a. To ensure that the entire sequence above is amplified, what 6-nucleotide DNA primers should you add to your PCR mix? You order the primers listed above, but instead receive the following set of primers: 5’-CGCATT-3’ 5’-GGACCT-3’ b. What portion of the double stranded DNA molecule will be amplified after 10 rounds of PCR? Your labmate attempts to rescue your PCR reaction by providing you with the following set of primers: 5’-ATACGC-3’ 5’-TCCTAA-3’ c. What is the result of running the PCR reaction with your labmate’s primers? How many double stranded molecules of DNA will result from 10 rounds of amplification?
- וןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…Task #2 Flow of information: A codon table is provided above. The 5' codon nucleotide is in the left column, and second codon nucleotide is on top. The Mcr1 M and m allele sequences are shown again in the central dogma grids below with the reading frame designated. Fill in the grids. In the second column indicate the end polarity (5' or 3', N or C). For both alleles, determine the sequences of the DNA template strand, mRNA, tRNA and polypeptide. Mc1r gene M allele: DNA sense strand DNA template strand mRNA codon tRNA anticodon polypeptide Mc1r gene m allele: DNA sense strand DNA template strand mRNA codon tRNA anticodon polypeptide 5' A A A A 5' A A A A A A Q C C G C A T G C A C A A CTask A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from cDNA extracted from different samples through PCR. You will need to run a total of 7 PCRs: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix—all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes. The table below lists the different components for PCR, the available stock concentrations of these components, and the needed working concentrations for the PCR itself. Complete the table by supplying the needed volumes of each component for a single reaction and for the master mix (the first row has been filled in as an example).
- Problem B. DNA: Codon SegmentingThe way that DNA is often interpreted as genes is in groups of three nucleotides at a time, called “codons.” Thus, the DNA strand dna_str = 'agctttcattctgac' Can be broken into codons in the following three ways: agc ttt cat tct gac a gct ttc att ctg ac ag ctt tca ttc tga c # reading frame 0 # reading frame 1 # reading frame 2 Notice that in these lines, we start reading codons at string indexes 0, 1 and 2. The three different start indices are known as reading frames, and are called reading frame 0, reading frame 1 and reading frame 2, respectively. It is not always clear which of these frames will be read by genetic transcription mechanisms, so it is often useful to be able to be flexible and consider any of them when working with DNA strands. Write a function segment that takes as an input a string containing a DNA strand, and a reading frame (0, 1 or 2) to use. The function should return a list containing the sequence of individual codons. You…Question: Genetically modified animal that might be approved for human consumption is a super “muscly” pig made by the inactivation of the myostatin gene. During normal development, the myostatin protein prevents the overgrowth of muscles. How would such a pig be achieved using CRISPR? Why would it not considered the GMO?question: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Biomimetic Engineering of Nanodelivery Systems: Artificial Viruses in the Making In an effort to engineer the next generation of nanoscale vectors, scientists have moved from using inorganic components aimed at obtaining inert structures to utilizing biological building blocks that are able to convey additional functionalities to the resulting construct. To cope with the complexity of the body and to evade the multiple layers of defense that tissues and organs have, it is critical to rely on the ability of certain materials to interact with, rather than to eschew, the biology of our body. Every NP system conceived to date faces one common fate: whether injected,…
- WORKSHEET TASK 3: 1. Below is a theoretical section of DNA. Design two primers that are 10 base pairs (bp) long that will amplify this section of DNA in a PCR reaction (‘N’ refers to non-specific ‘nucleotide’). 3’–A C G T G A A C T G C C T NNN......NNN C C G T G T A T C T C T T–5’ 5’–T G C A C T T G A C G G A NNN......NNN G G C A C A T A G A G A A–3’question: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Interference with Cellular Uptake, Immobilization, and Inactivation of the Virus Outside of the Host Cell Nanomaterials can be synthesized with a high specific surface area of a few hundred square meters per gram. Therefore, dependent on the surface properties, nanomaterials efficiently adsorb biomolecules and form a so-called biomolecular corona. This passive, nontargeted adsorption might be utilized to bind viruses, provided that the selected nanomaterial is relatively biocompatible. Viral surface proteins are often modified by sugar moieties or encompass positively charged amino acid patches that bind to lectins or glycosaminoglycans (GAGs) of heparan sulfate…Final Assessment for Exploring M X forms/d/e/1FAIpQLSe8RrfvHEr59pISGjbEneL041GxKTvyw9-Xc_EA7Um_8xqReA/viewform?hr_submission-Chg1990yzQ8SEAjwvY285... First mRNA base (5) end of codon) A Sequence C Sequence D 5. A DNA sequence of "ACG" will code for the amino acid UUG CUC CUA CUG wh GUC GJA GUG Cys 100 AUU AUC lle ACC AUA ACA AUG MACG] Val M Second mRNA base UCA UCG The GCU GCC.. GCA GIGA CAA CAG Tyr UAC UGC UAA Stop UGA Stop A UAG Stop UGG Tro AAU AAC MT AAC GAC GAG 2 Lys Asp CGA CGG AGU AGC AGA AGG Lys GGU GGC GGA GGG Arg DUAU Gly C Third mRNA base (3' end of codon) Lys (K) DELL C GU A C DEOTOCCAGUCAGUCAGUCHOSOTOS M G (F) A (LS1-1) * GU A C CUGA OPCUGACU GACUSACCO AD A G OCO Trp (W) \u« 9799%3Fo 33 4