Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce the overhangs in the cut DNA sample? Restriction Enzyme Specificities: ECORI .GAATTC. Hpal ..GTTAAC. Pst .CTGCAG. ..CAATTG... ..СТТААG... .GĄCGTC. Hind III. AAGCT... Bam HI .GGATC. .TTCGAA. .CCTAGG. DNA sample: GATCC- ICCTAG ... A Pstl в) Нраl EcoRI D Hindll E BamHI

Q: In 1-2 sentences each, state how the UvrA and rfa phenotypes make Ames test strains more sensitive…

A: Ames test This test uses various strains of bacteria that may carry a mutation. One of the commonly…

Q: digest suspect B's DNA using restriction enzyme 1. Restriction enzyme 1 cleaves the recognition site…

A: The restriction enzymes are endonucleases that digest DNA (deoxyribonucleic acid) molecules by…

Q: Define the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd.…

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Q: Single stranded DNA fragment of M13 phage DNA shown was mixed with the following  primers P1: AGTAG…

A: Polymerase chain reaction also abbreviated as PCR.A specific DNA fragment is quickly multiplied…

Q: Why is the restriction enzyme digestion step critical in 3C assays?

A: Genetic information is stored in DNA and we often consider the DNA molecule only as a linear…

Q: What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of…

A: Restriction fragment length polymorphism (RFLP) : it is a method used in genome mapping and…

Q: the restriction enzyme NotI recognizes the following sequence : 5'-GCGGCCGC-3' . On average, how…

A: Restriction enzymes are special enzymes that cut the DNA (Deoxyribonucleic acid) at a specified or…

Q: What is a restriction enzyme? What structure does it recognize?What type of chemical bond does it…

A: Werner Arber, a Swiss microbiologist, winner of 1978 Nobel Prize in Physiology, for his discovery…

Q: Based on the restriction enzyme specificities given below, which will generate blunt ends?…

A: Restrictions enzyme or restrictions endonucleases is type of protein, produces bacteria that helps…

Q: etion enzyme Sall cuts the sequence GTCGAC to leave a five base, 5' overhang. The restriction enzyme…

A: A restriction enzyme is classified under the category of a protein. These are isolated from bacteria…

Q: Describe how you can determine if the gene is interrupted, and if so the number of interruptions by…

A: Gel electrophoresis is a technique in which macromolecules such as DNA, RNA or proteins are broken…

Q: You have a DNA sequence of 1800 bp. To characterize it, you decide to make a restriction map.…

A: Restriction mapping is a process that helps to determine the location of the restriction site. The…

Q: A i. crossing over ii. biomarker a. Еcorl b. Recombinant iii. Gene-of-interest iv. restriction…

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Q: A. Avery, MacLeod and McCarty proved DNA is the genetic material. If you were to repeat their…

A: The DNA replication is heterocatalytic process by which a new DNA strand is synthesized on a old DNA…

Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: Fill in the blank spaces by selecting the correct enzyme for each of the restriction enzyme…

A: DNA  is cleaved to form DNA fragments with the help of an enzyme known as the restriction enzyme.…

Q: The DNA site of a person diagnosed with Sickle Cell Anemia will be:

A: Sickle cell anemia is a genetic disease which occurs due to substitution of glutamic acid by…

Q: gment shows where a number c between those cut sites in base Bem het Bem H 700 800 1000 1100

A: A restriction enzyme, also known as a restriction endonuclease or restrictase, is an enzyme that…

Q: Meselson and Stahl grew bacteria in medium containing heavy nitrogen. After many cell divisions, the…

A: From Meselson and Stahl's experiment we have known that DNA replication is semi-conservative.

Q: The detection of single nucleotide polymorphisms is possible with: O A. Restriction Enzymes O B. All…

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Q: Choose the combination of answers that most accurately completes the statement.Which of the…

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Q: B) Suppose, you had a linear DNA instead of circular DNA. The double stranded sequence of a…

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Q: Based on the restriction enzyme specificities given below, which will generate blunt ends?…

A: When a DNA fragment is subjected to the action of a restriction endonuclease enzymes, it cuts the…

Q: Why does Northern blotting not require the use of restriction enzymes?

A: Restriction enzyme naturally cleaves DNA because it's have double stranded sequence of neucleiotides…

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

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Q: Features of restriction enzymes include the following, except  __________ . a. They are produced…

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Q: You want to have E. coli take up your recombinant DNA vector you designed. What is the most likely…

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Q: When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with…

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Q: X ↓ O 650 O 550 O 500 200 O 850 Smal ✓ O 1550 300 Msel 350 Probe Smal ↓ Caption: Restriction enzyme…

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Q: You performed RADseq analysis of two genomes using the restriction enzyme EcoRI. From each genome,…

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Q: Identify the basic steps (in right order) in AFLP marker analysis. Explain briefly how this marker…

A: Basic steps in AFLP marker analysis.

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Q: What does it mean if a restriction enzyme produces ‘sticky’ or ‘blunt’ ends? Does the restriction…

A: The sticky or blunt ends refers to the edges of DNA molecules. The sticky ends tend to overlap to…

Q: What are the DNA extraction methods? Explain each one.

A: Ans: The Deoxyribonucleic acid (DNA) needs to be extracted out of cell for further studies on which…

Q: molecular scissors", justify that

A: Restriction enzymes are found in a wide variety of prokaryotes. Restriction enzymes received their…

Q: Restriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the…

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Q: 2. You are planning to clone a gene into the PBR322 plasmid vector. The gene has Bgl II restriction…

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Q: Consider the following:  Restriction enzymes bind and cut between bases T and A.  Based on this,…

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Q: The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted…

A: Hi, Thanks For Your Question.  Answer : Correct Option Is b (20%) Explanation :  P (III-7 Is…

Q: An 800 bp fragment is completely digested with a single restriction enzyme. Fragments of 50*, 150*,…

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Q: If this gene is methylated, which of the following combinations of DNA fragments would you expect…

A: Answer: RESTRICTION ENZYME = It is the enzyme which cuts the strands of DNA at specific sites…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.