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Analysis of Cell-Cycle Mutants in Yeast?
Step 1
Cell division cycle mutant in a yeast cell that has cell division cycle genes and has mutated to become sensitive to temperatures various parts of normal yeast cell cycle becomes abnormal and some yeast cell in strains do not survive at all.
Step by step
Solved in 2 steps
- Explain A cell-cycle mutant of yeast grown at restrictive temperature?Concerning the Tools of Genetics Box Analysis ofCell-Cycle Mutants in Yeast:a. Describe how you would use replica plating ofmutagenized, haploid yeast cells to identifytemperature-sensitive (ts) mutations in essentialgenes needed for yeast growth and survival.b. Among the many ts mutations you found in part(a), how would you distinguish mutations in genesneeded for cell-cycle progression from those ingenes needed for other aspects of the life of yeasts?c. If you had a large collection of yeast cell-cyclemutants, how would you determine which of themutations are in the same gene and which are indifferent genes?To identify genes controlling the cell cycle in budding yeast, a genetic screen was carried out. In this screen, haploid yeast cells were exposed to a DNA damaging agent to introduce random mutations in the genome. By culturing cells at an elevated temperature (e.g. 37 degrees), where many mutated genes lose their function, scientists identified yeast mutants that showed growth defects and arrest at specific stages of the cell cycle (e.g. in mitosis with large buds). In this screen, mutants of the cyclin-dependent kinase were identified, but not mutants of cyclins. Explain the reason for this outcome.
- Briefly describe three ways that ATP-dependent chromatin-remodeling complexes may change chromatin structure.What physiological differences between S. pombe and S. cerevisiae make them useful yet complementary tools for studying the molecular mechanisms involved in cell cycle regulation and control?Describe how the duplicated bacterial chromosomes are distributed into new daughter cells without the direction of the mitotic spindle.
- Explain the role of CDK inhibitors. If cyclin-CDK complexes are necessary to allow regulated progression through the eukaryotic cell cycle, what would be the physiological rationale for CDK inhibitors?Describe centromeres in yeastPart A and B A. What below would be likely to lead to cancer development? A) Overexpression of a cell cycle checkpoint inhibitor B) Loss of expression of a growth factor that promotes cell cycle entry C) Overexpression of a receptor tyrosine kinase that promotes cell cycle entry D) Overexpression of a DNA damage repair enzyme E) Loss of expression of a regulatory transcription factor that activates transcription of a cyclin B. Taxols inhibit the proper function of microtubules and are frequently used as chemotherapy drugs. What is the function of microtubules during the cell cycle? A) They promote the G1 to S checkpoint B) They normally inhibit M phase from being completed C) They form the mitotic spindle E) They mediate DNA replication F) They form the cleavage furrow during cytokinesis