Explain A cell-cycle mutant of yeast grown at restrictive temperature?

Create workplan step by step on regenerating a BT maize plant selecting using phosphinothricin herbicide. Mention uses of agrobacterium , somatic embryogenesis, and construction of engineered TDNA for your workplan

Mutants of cyclin B that are resistant to degradation by the cyclin B protease have been generated. How would the presence of these cyclin B mutants affect the events at the metaphase to anaphase transition?

name the enzymes of matrix biosynthesis, which should correct violations of the DNA structure in the norm, indicate its localization, connection with the phases of the cell cycle, energy sources

Please asap

Discuss and explain the molecular switches involve during the event of sporulation in bacterial cell.

What is the ploidy and equation for a typical S. pombe cell? Describe specifically how the ploidy of the yeast cells, and the phase in which the cells have been arrested, will increase the success of creating cells that will express the phenotype of the silenced target gene.

You need linear-linear paper and log-linear paper (also called semi-log paper), for linear-linear,10 squares per inch. For log-linear = semi-log you need at least 3 full cycles.   Plot the values below on each type of paper, then select two points marking an approximate doubling of cell density and interpolate back to the ‘x’ axis to determine the time. Indicate the bracketing for your estimate, and state ‘g= ____ hr’     Time (minutes)          OD420                   0                      0.091                          20                     0.092                                         40                    0.111                          60                     0.152                          80                     0.208          100                     0.303          120                     0.430          140                     0.577          160                     0.732         180                      0.902          200                     1.001          220…