In class, we talked about flow cytometry which allows scientists to determine what stage of the cell cycle cells are in by measuring their DNA content. Draw what the graph would look like for the mutant below at a 0-time point when there are 1000 cells total, with 5000 cells in G1, 350 cells in M and G2, and 150 cells in S phase. And then a graph of 1 hour later when the cells should have competed for mitosis: → loss of function of cdc25

Answered: In class, we talked about flow…

Human Biology (MindTap Course List)

11th Edition

ISBN:9781305112100

Author:Cecie Starr, Beverly McMillan

Publisher:Cecie Starr, Beverly McMillan

Chapter18: Cell Reproduction

Section: Chapter Questions

Problem 2CT: Suppose you have a way of measuring the amount of DNA in a single cell during the cell cycle. You...

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In class, we talked about flow cytometry which allows scientists to determine what stage of the cell cycle cells are in by measuring their DNA content. Draw what the graph would look like for the mutant below at a 0-time point when there are 1000 cells total, with 5000 cells in G1, 350 cells in M and G2, and 150 cells in S phase. And then a graph of 1 hour later when the cells should have competed for mitosis:

→ loss of function of cdc25

Expert Solution

Step 1

Cdc25 is a phosphatase enzyme having dual specificity that is they can act on serine/threonine as well as tyrosine residues. It was initially extracted as a cell cycle mutant from Schizosaccharomyces pombe which is a species of yeast. It controls the progression and entry into the different phases of the cell cycle including the synthesis phase and mitotic phase. It is an antagonist to Wee1 which inhibits the entry of cells into the mitotic phase.

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